Afyonkarahisar’da Yetiştirilen Holstein Sığırlarda Pit1/Hinfi Polimorfizmi

Year 2024, Volume: 17 Issue: 4, 386 - 392

Pelin Gezer Bilecen , Eda Demirtaş , Metin Erdoğan , Cevdet Uğuz

https://doi.org/10.30607/kvj.1485628

Abstract

Pit1 (Hipofize Özgü Transkripsiyon Faktörü-1) Sığırlarda 1.kromozomun sentromerik bölgesinde bulunan yaklaşık 129 aminoasitten oluşan bir protein kodlayan bir gendir. Memelilerde hipofiz bezinin gelişiminden ve hormon ekspresyonundan sorumlu olan Pit1 geni, POUF1F olarak da bilinmektedir. Pit1 eksikliğinde farelerde, köpeklerde, balıklarda cücelik fenotipi gözlenmektedir. Yapılan çalışmalar, Pit1 genindeki polimorfizmlerin sığırlarda, koyunlarda, domuzlarda, tavuklarda büyüme, üreme performansı, et ve süt verimi özellikleriyle ilişkili olduğunu göstermiştir. Sığırlarda Pit1/HinfI (c.1178G>A) polimorfizminin süt verimi ve döl verimi parametreleriyle ilişkili olduğu ifade edilmektedir. Bu çalışmada, Afyonkarahisar ilinde yetiştirilen 81 baş Holstein sığır Pit1/HinfI polimorfizminin varlığı ve dağılımının belirlenmesi amaçlanmıştır. PCR-RFLP tekniği kullanılarak hayvanların bu polimorfizm açısından genotiplendirilmiştir. Çalışma sonucunda 81 hayvandan 5 tanesinin AA, 30 tanesinin AG ve 46 tanesinin GG genotipinde olduğu bulunmuştur. G allelinin frekansı 0,75, A allelinin frekansı ise 0,25 olarak hesaplanmıştır. Genetik indeks değerlerinden PIC değeri 0,3027418 ve Heterozigotluk değeri 0,3742044 olarak hesaplanmıştır. χ2 değeri 0,049 bulunmuş olup, popülasyonun Hardy-Weinberg dengesinde olduğu ortaya konulmuştur. Bu bulgular ışığında Pit1/HinfI polimorfizminin Afyonkarahisar ilinde yetiştirilen Holstein sığırlar için markör destekli seleksiyon çalışmalarında kullanılabilecek potansiyel bir aday olduğu düşünülmektedir.

Keywords

HinfI, Holstein sığır, PCR-RFLP, Pit1

Pit1/Hinfi Polymorphism in Holstein Cattle in Afyonkarahisar Province

Abstract

Pit1 (Pituitary Specific Transcription Factor-1) gene has been shown to be resided on the centromeric region of chromosome 1 in cattle. This gene, also known as POUF1F has responsible for development of pituitary gland and hormone expression in mammals. Pit1 deficiency causes a dwarfism phenotype in mice, dogs and fish. Previous studies have demonstrated to associated with polymorphisms of the Pit1 gene and growth and reproductive performance and milk, meat yield traits. In the cattle, a HinfI polymorphism on Pit1 gene (c.1178G>A) have been reported that there is an association between milk and reproduction yield parameters. In this study, we aimed existence and distribution of Pit1/HinfI polymorphism in 81 head Holstein cattle in Afyonkarahisar province. In our study, we defined that there were 5 AA, 30 AG and 46 GG genotypes in our survey population. We also detected to frequency of A allele as 0.25 and the G allele as 0.75. We calculated to genetic index values such as PIC (0.3027418) and Heterozygosity (0.3742044) using RStudio package. Chi-square value was found 0.049 and was exhibited to survey population in Hardy-Weinberg equilibrium. The Pit1/HinfI polymorphism is a potential option for use in marker-assisted selection studies in light of these findings for Holstein cattle in Afyonkarahisar province.

Keywords

HinfI, Holstein sığır, PCR-RFLP, Pit1

Ethical Statement

All animal-related procedures were approved by the local ethic committee (approval AKUHADYEK 49533702/25) at Afyon Kocatepe University.

Supporting Institution

Afyon Kocatepe University, BAP coordinating

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