Research Article
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Investigation of the Relationship between IgG and Telomerase In Simental Cattle In Different Age Groups

Year 2021, Volume: 9 Issue: 2, 23 - 27, 31.08.2021
https://doi.org/10.24998/maeusabed.950176

Abstract

Preservation of telomere length suggests that it can play an active role in avoiding diseases that can be seen with aging and in avoiding cellular aging. Telomeres shorten slightly with each cell division. Telomerase enzyme is responsible for elongation of telomere sequences. Immunoglobulins can be thought of as the molecular structure of antibodies. IgG making up 75-80% of antibodies. The aim of this study is to evaluate the levels of IgG and telomerase enzyme, which have an important place in the immune system with aging. The research material consisted of a total of 46 animals of Simmental breed, 1-day-old 8 calves, 6-month-old 8calves, 1-year-old 8 calves, 4-year-old 12 and 6-year-old 10 cattle in various farms. After the blood samples taken from the animals were centrifuged, their serums were removed and telomerase enzyme levels and IgG levels were measured and compared by ELISA method. As a result, in this study, it was observed that telomerase enzyme activity decreases with aging. While IgG levels did not decrease in the first four groups, a positive correlation was found between IgG and telomerase values in 6-year-old animals. In addition, no significant difference was observed in animals in the same age groups.

Supporting Institution

Burdur Mehmet Akif Ersoy Üniversitesi, Bilimsel Araştırma Projeleri Birimi

Thanks

This study was supported by Mehmet Akif Ersoy University Scientific Research Projects Commission (Project No: 0582-YL-19).

References

  • Bergmann-Leitner, E.S., Mease, R.M., Duncan, E.H., Khan, F., Waitumbi, J., Angov, E., 2008. Evaluation of immunoglobulin purification methods and their impact on quality and yield of antigen specific antibodies. Malaria Journal 7, 129-138.
  • Blasco, M.A., 2005. Telomeres and Human Disease: Aging, Cancer and Beyond. Nature Reviews: Genetics 6, 611-22.
  • Brümmendorf, T.H., Mak, J., Sabo, K.M., Baerlocher, G.M., Dietz, K., Abkowitz, J.L., 2002. Lansdorp PM. Longitudinal studies of telomere length in feline blood cells: implications for hematopoietic stem cell turnover in vivo. Experimental Hematology 30, 1147-1152.
  • Chiu, C.P., Harley, C.B., 1997. Replicative senescence and cell immortality: The role of telomeres and telomerase. Proceeding of the Society for Experimental Biology and Medicine 214, 99-106.
  • Duncan, J.R., Wilie, B.N., Hiestand, F., Winter, A.J., 1972. The serum and secretory immunoglobulins of cattle: Characterization and quantitation. The Journal of Immunology 108, 965-976.
  • Effros, R.B., 2011. Telomere/telomerase dynamics within the human immune system: Effect of chronic infection and stress. Experimental Gerontology 46(2–3), 135–140.
  • Fali, T., Fabre-Mersseman, V., Bayard, C., Papagno, L., Fastenackels, S., Zoorab, R., Sauce, D., Appay, V., Yamamoto, T., Koup, R.A., Boddaert, J., 2018. Elderly human hematopoietic progenitor cells Express cellular senescence markers anda re more susceptible to pyroptosis. Jci Insight 3, 13.
  • Flannagan, K.S., Bowman, A.A., Mora-Plazas, M., Marin, C., Rentschler, K.M., Rozek, L.S., Villamor, E., 2020. Micronutrient status and leukocyte telomere length in school-age Colombian children. European Journal of Nutrition 59, 3, 1055- 1065.
  • Gershwin, L.J., 1990. The physiochemical and biological basis of immunity. In: E.L. Biberstein and Y. Chung Zee (Ed.), Review of Veterinary Microbiology. Boston, Oxford, London, Edinburgh, Melbourne: Blackwell Scientific Publications 9-39.
  • Giraudeau, M., Heidinger, B., Bonneaud, C., Sepp, T., 2019. Telomere shortening as a mechanism of long-term cost of infectious diseases in natural animal populations. Bıology Letters 15, 5.
  • Harley, C.B., 1997. Human ageing and telomeres. In: Telomeres and Telomerase, Ed.: D.J. Chadwick, G. Cardew, West Sussex: John Wiley and Sons, 129-147. Jose, S.S., Bendickova, K., Kepak, T., Krenova, Z., Fric, J., 2017. Chronic inflammation in immune Aging: Role of pattern recognition receptor crosstalk with the telomere complex?. Frontıers in Immunology 8.
  • Kacskovıcs, I., Kis, Z., Mayer, B., West, A.P., Tiangco, N.E., Tılahun, M., Cervenak, L., Bjorkman, P.J., Goldsby, R.A., Szencı, O., Hammarström, L., 2006. FcRn mediates elongated serum half-life of human IgG in cattle. International Immunology 10, 1-12.
  • Klaus, G.G., Bennet, B.A., Jones. E.W., 1969. A quantitative study of the transfer of colostral immunoglobulins to the newborn calf. Immunology 16, 293-299.
  • Kol, A., 2008. Human intravenous immunoglobulin (IVIG) therapy in veterinary medicine-A review. Israel Journal of Veterinary Medicine 63 (1), 23-27.
  • Mehra, R., Marnıla, P., Korhonen, H., 2006. Milk immunoglobulins for health promotion. International Dairy Journal 16, 1262-1271.
  • Miller, R.A., 2000. Telomere diminution as a cause of immune failure in old age: an unfashionable demurral. Biochem Soc Trans 28(2), 241–5.
  • Richard son, R.B., Allan, D.S., Le, Y. 2014. Greater organ involution in highly proliferative tissues associated with the early onset and acceleration of ageing in humans. Experimental Gerontology 55, 80-91.
  • Roy, J.H.B., 1980. Factors affecting susceptibility of calves to disease. Journal of Dairy Science 63, 650-664.
  • Selk, G.E., 2000. Disease Protection for Baby Calves. Oklahoma Cooperative Extension Service F-3358.
  • Tarry-Adkins, J.L., Aiken, C.E., Ashmore, T.J., Ozanne, S.E., Spiroski, A.M., Sutherland, M.J., Camm, E.J., Giussani, D.A., Rolfo, A., 2019. A suboptimal maternal diet combined with accelerated postnatal growth results in an altered aging profile in the thymus of male rats. Faseb Journal 33, 1, 239-253.
  • Tizard, I.R., 2004. Veterinary Immunology: An Introduction. (7th ed.). Philadelphia, Pennsylvania: Saunderes.
  • Von Zglinicki, T., Pilger, R., Sitte, N., 2000. Accumulation of single-strand breaks is the major cause of telomere shortening in human fibroblasts. Free Radical Biology and Medicine 28, 64–74.
  • Wilkie, B.N., 1974. Review of Bovine Immunology for the Veterinary Practitioner. The Canadian Veterinary Journal 15(9), 243-248.

Investigation of the Relationship between IgG and Telomerase In Simental Cattle In Different Age Groups

Year 2021, Volume: 9 Issue: 2, 23 - 27, 31.08.2021
https://doi.org/10.24998/maeusabed.950176

Abstract

Preservation of telomere length suggests that it can play an active role in avoiding diseases that can be seen with aging and in avoiding cellular aging. Telomeres shorten slightly with each cell division. Telomerase enzyme is responsible for elongation of telomere sequences. Immunoglobulins can be thought of as the molecular structure of antibodies. IgG making up 75-80% of antibodies. The aim of this study is to evaluate the levels of IgG and telomerase enzyme, which have an important place in the immune system with aging. The research material consisted of a total of 46 animals of Simmental breed, 1-day-old 8 calves, 6-month-old 8calves, 1-year-old 8 calves, 4-year-old 12 and 6-year-old 10 cattle in various farms. After the blood samples taken from the animals were centrifuged, their serums were removed and telomerase enzyme levels and IgG levels were measured and compared by ELISA method. As a result, in this study, it was observed that telomerase enzyme activity decreases with aging. While IgG levels did not decrease in the first four groups, a positive correlation was found between IgG and telomerase values in 6-year-old animals. In addition, no significant difference was observed in animals in the same age groups.

References

  • Bergmann-Leitner, E.S., Mease, R.M., Duncan, E.H., Khan, F., Waitumbi, J., Angov, E., 2008. Evaluation of immunoglobulin purification methods and their impact on quality and yield of antigen specific antibodies. Malaria Journal 7, 129-138.
  • Blasco, M.A., 2005. Telomeres and Human Disease: Aging, Cancer and Beyond. Nature Reviews: Genetics 6, 611-22.
  • Brümmendorf, T.H., Mak, J., Sabo, K.M., Baerlocher, G.M., Dietz, K., Abkowitz, J.L., 2002. Lansdorp PM. Longitudinal studies of telomere length in feline blood cells: implications for hematopoietic stem cell turnover in vivo. Experimental Hematology 30, 1147-1152.
  • Chiu, C.P., Harley, C.B., 1997. Replicative senescence and cell immortality: The role of telomeres and telomerase. Proceeding of the Society for Experimental Biology and Medicine 214, 99-106.
  • Duncan, J.R., Wilie, B.N., Hiestand, F., Winter, A.J., 1972. The serum and secretory immunoglobulins of cattle: Characterization and quantitation. The Journal of Immunology 108, 965-976.
  • Effros, R.B., 2011. Telomere/telomerase dynamics within the human immune system: Effect of chronic infection and stress. Experimental Gerontology 46(2–3), 135–140.
  • Fali, T., Fabre-Mersseman, V., Bayard, C., Papagno, L., Fastenackels, S., Zoorab, R., Sauce, D., Appay, V., Yamamoto, T., Koup, R.A., Boddaert, J., 2018. Elderly human hematopoietic progenitor cells Express cellular senescence markers anda re more susceptible to pyroptosis. Jci Insight 3, 13.
  • Flannagan, K.S., Bowman, A.A., Mora-Plazas, M., Marin, C., Rentschler, K.M., Rozek, L.S., Villamor, E., 2020. Micronutrient status and leukocyte telomere length in school-age Colombian children. European Journal of Nutrition 59, 3, 1055- 1065.
  • Gershwin, L.J., 1990. The physiochemical and biological basis of immunity. In: E.L. Biberstein and Y. Chung Zee (Ed.), Review of Veterinary Microbiology. Boston, Oxford, London, Edinburgh, Melbourne: Blackwell Scientific Publications 9-39.
  • Giraudeau, M., Heidinger, B., Bonneaud, C., Sepp, T., 2019. Telomere shortening as a mechanism of long-term cost of infectious diseases in natural animal populations. Bıology Letters 15, 5.
  • Harley, C.B., 1997. Human ageing and telomeres. In: Telomeres and Telomerase, Ed.: D.J. Chadwick, G. Cardew, West Sussex: John Wiley and Sons, 129-147. Jose, S.S., Bendickova, K., Kepak, T., Krenova, Z., Fric, J., 2017. Chronic inflammation in immune Aging: Role of pattern recognition receptor crosstalk with the telomere complex?. Frontıers in Immunology 8.
  • Kacskovıcs, I., Kis, Z., Mayer, B., West, A.P., Tiangco, N.E., Tılahun, M., Cervenak, L., Bjorkman, P.J., Goldsby, R.A., Szencı, O., Hammarström, L., 2006. FcRn mediates elongated serum half-life of human IgG in cattle. International Immunology 10, 1-12.
  • Klaus, G.G., Bennet, B.A., Jones. E.W., 1969. A quantitative study of the transfer of colostral immunoglobulins to the newborn calf. Immunology 16, 293-299.
  • Kol, A., 2008. Human intravenous immunoglobulin (IVIG) therapy in veterinary medicine-A review. Israel Journal of Veterinary Medicine 63 (1), 23-27.
  • Mehra, R., Marnıla, P., Korhonen, H., 2006. Milk immunoglobulins for health promotion. International Dairy Journal 16, 1262-1271.
  • Miller, R.A., 2000. Telomere diminution as a cause of immune failure in old age: an unfashionable demurral. Biochem Soc Trans 28(2), 241–5.
  • Richard son, R.B., Allan, D.S., Le, Y. 2014. Greater organ involution in highly proliferative tissues associated with the early onset and acceleration of ageing in humans. Experimental Gerontology 55, 80-91.
  • Roy, J.H.B., 1980. Factors affecting susceptibility of calves to disease. Journal of Dairy Science 63, 650-664.
  • Selk, G.E., 2000. Disease Protection for Baby Calves. Oklahoma Cooperative Extension Service F-3358.
  • Tarry-Adkins, J.L., Aiken, C.E., Ashmore, T.J., Ozanne, S.E., Spiroski, A.M., Sutherland, M.J., Camm, E.J., Giussani, D.A., Rolfo, A., 2019. A suboptimal maternal diet combined with accelerated postnatal growth results in an altered aging profile in the thymus of male rats. Faseb Journal 33, 1, 239-253.
  • Tizard, I.R., 2004. Veterinary Immunology: An Introduction. (7th ed.). Philadelphia, Pennsylvania: Saunderes.
  • Von Zglinicki, T., Pilger, R., Sitte, N., 2000. Accumulation of single-strand breaks is the major cause of telomere shortening in human fibroblasts. Free Radical Biology and Medicine 28, 64–74.
  • Wilkie, B.N., 1974. Review of Bovine Immunology for the Veterinary Practitioner. The Canadian Veterinary Journal 15(9), 243-248.

Details

Primary Language English
Subjects Health Care Administration
Journal Section Research Article
Authors

Serkan ÇETİNER This is me 0000-0002-0969-936X

Şima ŞAHİNDURAN 0000-0002-7718-2020

Project Number 0582-YL-19
Publication Date August 31, 2021
Submission Date June 10, 2021
Published in Issue Year 2021 Volume: 9 Issue: 2

Cite

APA ÇETİNER, S., & ŞAHİNDURAN, Ş. (2021). Investigation of the Relationship between IgG and Telomerase In Simental Cattle In Different Age Groups. Mehmet Akif Ersoy Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi, 9(2), 23-27. https://doi.org/10.24998/maeusabed.950176
AMA ÇETİNER S, ŞAHİNDURAN Ş. Investigation of the Relationship between IgG and Telomerase In Simental Cattle In Different Age Groups. Mehmet Akif Ersoy Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi. August 2021;9(2):23-27. doi:10.24998/maeusabed.950176
Chicago ÇETİNER, Serkan, and Şima ŞAHİNDURAN. “Investigation of the Relationship Between IgG and Telomerase In Simental Cattle In Different Age Groups”. Mehmet Akif Ersoy Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi 9, no. 2 (August 2021): 23-27. https://doi.org/10.24998/maeusabed.950176.
EndNote ÇETİNER S, ŞAHİNDURAN Ş (August 1, 2021) Investigation of the Relationship between IgG and Telomerase In Simental Cattle In Different Age Groups. Mehmet Akif Ersoy Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi 9 2 23–27.
IEEE S. ÇETİNER and Ş. ŞAHİNDURAN, “Investigation of the Relationship between IgG and Telomerase In Simental Cattle In Different Age Groups”, Mehmet Akif Ersoy Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi, vol. 9, no. 2, pp. 23–27, 2021, doi: 10.24998/maeusabed.950176.
ISNAD ÇETİNER, Serkan - ŞAHİNDURAN, Şima. “Investigation of the Relationship Between IgG and Telomerase In Simental Cattle In Different Age Groups”. Mehmet Akif Ersoy Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi 9/2 (August 2021), 23-27. https://doi.org/10.24998/maeusabed.950176.
JAMA ÇETİNER S, ŞAHİNDURAN Ş. Investigation of the Relationship between IgG and Telomerase In Simental Cattle In Different Age Groups. Mehmet Akif Ersoy Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi. 2021;9:23–27.
MLA ÇETİNER, Serkan and Şima ŞAHİNDURAN. “Investigation of the Relationship Between IgG and Telomerase In Simental Cattle In Different Age Groups”. Mehmet Akif Ersoy Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi, vol. 9, no. 2, 2021, pp. 23-27, doi:10.24998/maeusabed.950176.
Vancouver ÇETİNER S, ŞAHİNDURAN Ş. Investigation of the Relationship between IgG and Telomerase In Simental Cattle In Different Age Groups. Mehmet Akif Ersoy Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi. 2021;9(2):23-7.