Mantar Dergisi 2147-6845 Selcuk University Orpin ve Menke Besi Ortamlarında Neocallimastix sp'nin Selülotik Enzim Aktivitelerinin Karşılaştırılması Comparing Cellulotic Enzyme Activities of Neocallimastix sp. in Orpin’s and Menke’s Media https://orcid.org/0000-0003-4918-7796 Karaman Altuğ MUNZUR UNIVERSITY https://orcid.org/0000-0002-6196-5303 Yücel Halit KAHRAMANMARAS SUTCU IMAM UNIVERSITY https://orcid.org/0000-0002-0877-1358 Ekinci Kübra ATATURK UNIVERSITY https://orcid.org/0000-0003-4850-6927 Cömertpay Sabahattin KAHRAMANMARAS SUTCU IMAM UNIVERSITY 04 28 2022 13 1 55 61 01 04 2022 02 04 2022 Copyright © 2010, The Journal of Fungus 2010 The Journal of Fungus

Neocallimastix sp., benzersiz lignoselülotik enzimlere sahip Anaerobik Gut Fungusları (AGF) ailesinin bir üyesidir ve genellikle in vitro olarak iki temel besi ortamında da yetiştirilir. Enzimatik aktivitelerin tespiti için Orpin besi ortamı tercih edilirken, fermantasyon sırasında oluşan gazların değerlendirilmesi için daha çok Menke besi ortamı tercih edilmektedir. Bu iki ortamın hedeflenen amaçlar için etkili olduğu gösterilmiş olmasına rağmen, bu besi ortamlarındaki çeşitli selülotik enzimlerin aktivitelerini karşılaştırmak için hiçbir girişimde bulunulmamıştır. Bu çalışmada Neocallimastix sp.'nin bu iki besiyerinde de 7 güne kadar büyüme hızı ölçülmüş ve her ikisinde de benzer bir gelişim gözlenmiştir (p>0.05). Ayrıca Menke besi ortamının Aviselaz’ın 5. (p

Neocallimastix sp. is a member of the Anaerobic Gut Fungi (AGF) family with unique lignocellulotic enzymes, and it is usually grown in two basic media in vitro. Orpin’s mediais preferred for the detection of enzymatic activites while Menke’s is mostly chosen for evaluating the gases produced during the fermantation. Although these two media were shown to be effective for their targeted purposes, no attempt has been made to compare the activities of various cellulotic enzymes in them. In this study, we measured the growth rate of Neocallimastix sp. in these two media up to 7 days and A similar progress was observed in both (p>0.05). We also discovered that Menke’s media was better for the 5th (p

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