Mantar Dergisi 2147-6845 Selcuk University 10.30708/mantar.1909607 Plant and Fungus Systematics and Taxonomy Mycology Bitki ve Mantar Sistematiği ve Taksonomi Mikoloji Çam Kozalaklarında Hemimycena lactea: Türkiye'de İlk Moleküler Doğrulama Hemimycena lactea on Pine Cones: First Molecular Confirmation in Türkiye https://orcid.org/0000-0002-6049-4896 Acar İsmail YÜZÜNCÜ YIL ÜNİVERSİTESİ, BAŞKALE MESLEK YÜKSEKOKULU https://orcid.org/0000-0002-1778-2200 Karabıyık Halide TRAKYA ÜNİVERSİTESİ 04 30 2026 17 1 67 76 03 14 2026 04 17 2026 Copyright © 2010, The Journal of Fungus 2010 The Journal of Fungus

Bu çalışmada, Çanakkale (Yapıldık Köyü, Derinkoy mevkii) lokalitesinden çam kozalağı üzerinde 28.01.2025 tarihinde toplanan Hemimycena lactea (Acar 2041) örneği morfolojik ve moleküler verilerle incelenmiştir. Tür; tamamen beyaz ve narin basidiomaları, translusent-striat pileus kenarı, seyrek lamelleri ve ince stipesi ile literatürdeki tanımıyla uyumludur. Mikroskobik olarak elipsoidal–subfusiform, inamiloid sporlar (Q ≈ 2), 2–4 sterigmalı basidiler, kıskaç bağlantıları ve divertiküllü pileipellis yapısı belirlenmiştir. ITS rDNA dizisine dayalı Bayesyen filogenetik analizde (29 sekans; 27 iç grup, 2 dış grup) bu çalışmada elde edilen dizi (GenBank: PZ091910), referans H. lactea dizileriyle yüksek destekli (PP = 1.00) monofiletik bir klad içerisinde yer almıştır. Sonuçlar, tür teşhisini moleküler olarak doğrulamakta ve örneğin kış döneminde konifer kozalağı üzerinde gelişmesi, türün ekolojik toleransına ilişkin yeni veriler sunmaktadır.

In this study, a specimen of Hemimycena lactea (Acar 2041) was examined using morphological and molecular data. The specimen was collected on a pine cone from Çanakkale (Yapıldık Village, Derinkoy area) on January 28, 2025. The species is consistent with its description in the literature, with its completely white and delicate basidiomas, translucent-striate pileus margin, sparse lamellae, and thin stipe. Microscopically, the spores were found to be ellipsoidal–subfusiform in shape, and the spore wall was found to be inamyloid (Q ≈ 2). Microscopically, basidia with 2–4 sterigmata, clamp connections, and diverticulate-like pileipellis elements were observed; the spores were ellipsoidal–subfusiform and inamyloid (Q ≈ 2). In the context of Bayesian phylogenetic analysis, based on the ITS rDNA sequence (29 sequences; 27 in-groups, 2 out-groups), the sequence obtained in this study (GenBank: PZ091910) was placed within a highly supported (PP = 1.00) monophyletic clade with reference H. lactea sequences. The molecular results obtained from this study serve to confirm the species identification, and the observation of its growth on conifer cones during the winter period provides novel data on the ecological tolerance of the species.

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