Mantar Dergisi

2147-6845

Selcuk University

Kültüre Alınmış Lentinula edodes’in Metanol Ekstraktının Antioksidan ve İn Vitro Bazı Enzim İnhibitör Aktiviteleri

Antioxidant and In Vitro Some Enzyme Inhibitory Activities of Methanolic Extract of Cultivated Lentinula edodes

Alkan

Sinan

Özparlak

Haluk

SELÇUK ÜNİVERSİTESİ, FEN FAKÜLTESİ, BİYOLOJİ BÖLÜMÜ Zengin

Gökhan

Kaşık

Gıyasettin

10 31 2017

8 2 90 98 06 05 2017 08 10 2017

2010

The Journal of Fungus

Shiitake mantarı adıyla dabilinen Lentinula edodes (Berk.) Pegler Doğu Asya’ya özgü yenilebilirbir mantar olup antioksidan ve antimikrobiyal özellikleriyle tedavi edicietkiler içermektedir. Bu çalışmanın ilk kısmı Türkiye’deki kültür L. edodes’inmetanolik ekstraktının antioksidan aktivitesini ayrıca total fenolik ve flavonoidiçeriğini ilk kez tespit etmeyi amaçladı. Çalışmanın ikinci kısmında, bu mantarekstraktının kolinesteraz, tirozinaz, amilaz ve glukozidaza karşı enzim inhibitörpotansiyelleri araştırıldı. Bu çalışmada rutin çalışmalardan farklı olarak ultrasonik ekstraksiyon yöntemi kullanıldı.Ekstraktakitotal fenolik ve flavonoid içerikleri de Folin-Ciocalteu ve AlCl3 yöntemleriyletespit edildi. Antioksidan aktiviteleri serbest radikal süpürme (DPPH ve ABTS),indirgeme gücü (FRAP ve CUPRAC), fosfomolibdat ve metal şelatlama testleri gibifarklı yöntemlerle araştırıldı. In vitro enzim inhibitör potansiyelleribir mikroplate okuyucuyla ölçüldü. Bu çalışmada flavonoid içerik ve tirozinazenzim inhibisyonu tespit edilemedi. Ayrıca L. edodes ekstraktınınantioksidan aktivitesi ve enzim inhibisyonu verilerinin özellikle, popüler ve etkili bir başka tıbbi mantar olan Ganodermalucidum’un ekstraktından eldeedilen verilerden daha düşük olduğu dikkat çekti.

Lentinula edodes (Berk.) Pegler, as called Shiitake mushroom, is an edible fungus native to East Asia and contains several therapeutic actions such as antioxidant and antimicrobial properties. The first part of this work aimed to evaluate the antioxidant activity of methanolic extract of cultivated L. edodes in Turkey for the first time, as well as to measure the content of total phenolic and flavonoid compounds of mushroom extract. In the second part of this work, the enzyme inhibitory potentials of this mushroom extract were investigated against cholinesterase, tyrosinase, amylase and glucosidase. In this study, ultrasonication assisted extraction method was used in contrast to routine methods. Total phenolics and flavonoids contents in the extract were determined by Folin-Ciocalteu and AlCl assays.Antioxidant activities were investigated by using different assays, including free radical scavenging assays (DPPH and ABTS) reducing power (FRAP and CUPRAC), phosphomolybdenum and metal chelating assays. The in vitro enzyme inhibitory potentials were measured with a microplate reader. Flavonoid content and tyrosinase enzyme inhibition could not be detected in the present study. It has been noted especially that the antioxidant activity and enzyme inhibition data of the L. edodes extract were lower than those of the Ganoderma lucidum, another popular and effective medical mushroom.

Antioksidan kültür Lentinula edodes enzim inhibisyonu

Antioxidant cultivated Lentinula edodes enzyme inhibition

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