Mantar Dergisi 2147-6845 Selcuk University Preliminary Study on the Determination of Heat Resistant Fungi in Agricultural Soils Tarım Topraklarındaki Isıya Dirençli Fungusların Belirlenmesi Üzerine Ön Çalışma https://orcid.org/0000-0002-5901-5747 Sezen Suat Eskişehir Teknik Üniversitesi https://orcid.org/0000-0001-6702-489X Demirel Rasime Eskişehir Teknik Üniversitesi 12 26 2019 10 3 60 66 10 11 2019 12 08 2019 Copyright © 2010, The Journal of Fungus 2010 The Journal of Fungus

Heatresistant fungi are capable of surviving temperature at short heatapplications. These organisms continue to growing and metabolic activities.Therefore, they cause spoilage of food products and effect on health because ofmycotoxin production. This study focused on determination of density andbiodiversity of heat resistant fungi in agricultural soil samples. For thisaim, two different soil samples were collected from agricultural lands at June2018. Isolation process was performed by using main soil dilution method afterheat treatment at 75°C for 30 minutes of diluted soil samples. After incubationat 25°C for 7-14 days, all colonies were purified. All of the isolates werediagnosed by using conventional methods according to the macromorphological andmicromorphological properties. We determined 11 species belong to Alternaria, Aspergillus, Cladosporium,Epicoccum, Humicola, Penicillium andTaloromyces genera. As a result ofthis study, we showed that agricultural soil areas have a potential for heatresistance fungi biodiversity and exhibited that we should concentrate on thissubject with investigation of different areas and sources.

Isıya dayanıklımantarlar, kısa ısı uygulamalarına dayanabilir. Bu organizmalar büyümeye vemetabolik aktivitelere devam ederler. Bu nedenle, gıda ürünlerinde bozulmaya vemikotoksin üretme yetenekleri sayesinde sağlık üzerinde etkiye neden olurlar.Bu çalışma, tarımsal toprak örneklerinde ısıya dirençli mantarların yoğunluğuve biyolojik çeşitliliğinin belirlenmesi üzerine odaklanmıştır. Bu amaçla,Haziran 2018'de tarım alanlarından iki farklı toprak örneği toplanmıştır.İzolasyon işlemi dilüe edilmiş toprak örneklerinin 75 °C’de 30 dakika boyuncaısı uygulaması yapıldıktan sonra toprak dilüsyon yöntemi ilegerçekleştirilmiştir. 25 °C’de 7-14 günlük inkübasyondan sonra tüm kolonilersaflaştırılmıştır. İzolatların tamamı makromorfolojik ve mikromorfolojiközelliklere göre geleneksel yöntemler kullanılarak teşhis edilmiştir. Alternaria, Aspergillus, Cladosporium,Epicoccum, Humicola, Penicillium ve Taloromycescinslerine ait 11 tür tespit edilmiştir. Bu çalışma sonucunda, tarımsal toprakalanlarının ısıya dirençli mantar biyolojik çeşitliliği potansiyeline sahipolduğunu belirledik ve farklı alanların ve kaynakların araştırılmasıyla bukonuya odaklanmamız gerektiğini gösterdik.

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