Determination of Anti-MRSA and Antimicrobial Activity of Ramaria aurea Extracts

Year 2024, , 79 - 86, 14.10.2024

Erdoğan Güneş , Sinan Alkan

https://doi.org/10.30708/mantar.1514853

Abstract

Infectious diseases have been one of the leading causes of death worldwide for years. Especially multidrug-resistant Methicillin-Resistant Staphylococcus aureus (MRSA) strains are among the most important threats to public health worldwide. Basidiomycetes group fungi are known as a promising source of antibacterial compounds. This study aimed to determine the antimicrobial activity of methanol and ethanol extracts of Ramaria aurea (Schaeff.) Quél. against pathogenic standard microorganisms and anti-MRSA activity against MRSA strains isolated from clinical samples. Broth microdilution method was used to determine the antimicrobial activity. Concentrations between 12.5-0.006 mg/ml of the extracts were applied to the strains used. While methanol and ethanol extracts were found to have antibacterial activity against standard bacterial strains at doses ranging from 0.19 to 6.25 mg/ml, the most effective strain of both extracts was Bacillus cereus at a dose of 0.19 mg/ml. In addition, no antifungal effect was determined for both methanol and ethanol extracts. While methanol extract was found to have anti-MRSA activity against all studied MRSA strains at doses ranging from 0.04 to 6.25 mg/ml, ethanol extract was found to have anti-MRSA activity against all these strains at doses ranging from 0.19 to 3.12 mg/ml. As a result, it was determined that the studied Ramaria aurea extracts had antibacterial and anti-MRSA activity, and it can be said that this species may be a natural source of antimicrobial agents and may be used in the pharmaceutical industry.

Keywords

Antifungal, Antimicrobial, Anti-MRSA activity, Basidiomycota, Methicillin Resistant Staphylococcus aureus

Ramaria aurea Ekstraktlarının Anti-MRSA ve Antimikrobiyal Aktivitesinin Belirlenmesi

Abstract

Bulaşıcı hastalıklar yıllardan beri dünya çapında ölümlerin önde gelen nedenlerinden biridir. Özellikle çoklu ilaç direncine sahip Metisiline Dirençli Staphylococcus aureus (MRSA) suşları, dünya çapında halk sağlığını tehdit eden en önemli unsurların başında gelir. Basidiomycetes grubu mantarlar umut verici bir antibakteriyel bileşik kaynağı olarak bilinmektedirler. Bu çalışmada Ramaria aurea (Schaeff.) Quél.’nın metanol ve etanol ekstraktlarının patojen standart mikroorganizmalara karşı antimikrobiyal ve klinik örneklerden izole edilen MRSA suşlarına karşı anti-MRSA aktivitesinin belirlenmesi amaçlanmıştır. Antimikrobiyal aktiviteyi belirlemek için sıvı mikrodilüsyon metodu kullanılmıştır. Kullanılan suşlara ekstraktların 12.5-0.006 mg/ml arasındaki konsantrasyonları uygulanmıştır. Metanol ve etanol ekstraktlarının, standart bakteri suşlarına karşı 0.19-6.25 mg/ml aralığındaki dozlarda antibakteriyal aktiviteye sahip olduğu tespit edilirken, her iki ekstraktın da en etkili olduğu suşun 0.19 mg/ml dozla Bacillus cereus suşu olduğu görülmüştür. Ayrıca metanol ve etanol ekstraklarının her ikisinin de antifungal bir etkisi belirlenememiştir. Metanol ekstraktının çalışılan tüm MRSA suşlarına karşı 0.04-6.25 mg/ml aralığındaki dozlarda anti-MRSA aktivitesi gösterdiği belirlenirken, etanol ekstraktının bu suşların tamamına karşı 0.19-3.12 mg/ml aralığındaki dozlarda anti-MRSA aktivitesine sahip olduğu tespit edilmiştir. Sonuç olarak çalışılan Ramaria aurea ekstraklarının antibakteriyal ve anti-MRSA etkiye sahip olduğu belirlenmiş olup bu türün antimikrobiyal ajanların doğal bir kaynağı olabileceği ve farmakoloji endüstrisinde kullanılabileceği söylenebilir.

Keywords

Antifungal, Antimikrobiyal, Anti-MRSA aktivite, Basidiomycota, Metisilin dirençli Staphylococcus aureus

References

• Alves, M. J., Ferreira, I. C., Martins, A. ve Pintado, M., (2012). Antimicrobial activity of wild mushroom extracts against clinical isolates resistant to different antibiotics, Journal of Applied Microbiology, 113 (2), 466-475.
• Bal, C., Eraslan, E. C. ve Sevindik, M., (2023). Antioxidant, antimicrobial activities, total phenolic and element contents of wild edible mushroom bovista nigrescens, Prospects in Pharmaceutical Sciences, 21 (2), 37-41.
• Bala, N., Aitken, E. A., Fechner, N., Cusack, A. ve Steadman, K. J., (2011). Evaluation of antibacterial activity of Australian basidiomycetous macrofungi using a high-throughput 96-well plate assay, Pharmaceutical biology, 49 (5), 492-500.
• Belyurt, S. Ç., (2014). Gaziantap yöresinde yetişen bazı makromantar türlerinin antioksidan ve antimikrobiyal etkilerinin incelenmesi, Karamanoğlu Mehmetbey Üniversitesi.
• Betts, J. W. ve Wareham, D. W., (2014). In vitro activity of curcumin in combination with epigallocatechin gallate (EGCG) versus multidrug-resistant Acinetobacter baumannii, BMC microbiology, 14, 1-5.
• Breitenbach J., Kränzlin F., (1986). Fungi of Switzerland (Volume 2). Andrlag Mykologia.
• De Silva, D. D., Rapior, S., Sudarman, E., Stadler, M., Xu, J., Aisyah Alias, S. ve Hyde, K. D., (2013). Bioactive metabolites from macrofungi: ethnopharmacology, biological activities and chemistry, Fungal Diversity, 62, 1-40.
• Ellis M.B., Ellis J.P., (1990). Fungi Without Gills (Hymenomycetes and Gasteromycetes), Chapman and Hill, London.
• Eraslan, E. C., Altuntas, D., Baba, H., Bal, C., Akgül, H., Akata, I. ve Sevindik, M., (2021). Some biological activities and element contents of ethanol extract of wild edible mushroom Morchella esculenta, Sigma Journal of Engineering and Natural Sciences, 39 (1), 24-28.
• Fernandes, J. C., Tavaria, F. K., Soares, J. C., Ramos, O. S., Monteiro, M. J., Pintado, M. E. ve Malcata, F. X., (2008). Antimicrobial effects of chitosans and chitooligosaccharides, upon Staphylococcus aureus and Escherichia coli, in food model systems, Food microbiology, 25 (7), 922-928.
• Gezer, K., Duru, M. E., Kivrak, I., Turkoglu, A., Mercan, N., Turkoglu, H. ve Gulcan, S., (2006). Free-radical scavenging capacity and antimicrobial activity of wild edible mushroom from Turkey, African journal of Biotechnology, 5 (20).
• Hemaiswarya, S., Kruthiventi, A. K. ve Doble, M., (2008). Synergism between natural products and antibiotics against infectious diseases, Phytomedicine, 15 (8), 639-652.
• Janeš, D., Kreft, S., Jurc, M., Seme, K. ve Štrukelj, B., (2007). Antibacterial activity in higher fungi (mushrooms) and endophytic fungi from Slovenia, Pharmaceutical biology, 45 (9), 700-706.
• Kalač, P., (2009). Chemical composition and nutritional value of European species of wild growing mushrooms: A review, Food chemistry, 113 (1), 9-16.
• Kaşık, G. (2010). Mantar Bilimi: Marifet Matbaa ve Kâğıtçılık.
• Kaur, H., Sharma, S., Khanna, P. ve Kapoor, S., (2015). Evaluation of Ganoderma lucidum strains for the production of bioactive components and their potential use as antimicrobial agents, Journal of Applied and Natural Science, 7 (1), 298-303.
• Kim, K.-H., Han, S.-R., Kim, B.-L., Jung, S.-H. ve Oh, T.-J., (2017). Antimicrobial activities of Ramaria botrytis (Fr.) against oral bacteria, Journal of korean society of dental hygiene, 17 (3), 493-504.
• Kumar Sharma, S. ve Gautam, N., (2017). Chemical and bioactive profiling, and biological activities of coral fungi from northwestern Himalayas, Scientific reports, 7 (1), 46570.
• Liu, K., Wang, J., Zhao, L. ve Wang, Q., (2013). Anticancer, antioxidant and antibiotic activities of mushroom Ramaria flava, Food and chemical toxicology, 58, 375-380.
• Mahady, G. B., (2005). Medicinal plants for the prevention and treatment of bacterial infections, Current pharmaceutical design, 11 (19), 2405-2427.
• Ogbole, O. O., Adebisi, P. ve Segun, P. A., (2019). Medicinal mushrooms with methicillin resistant Staphylococcus aureus (MRSA) inhibitory activity, Journal of Pharmacy & Bioresources, 16 (1), 41-46.
• Okwu, M. U., Olley, M., Akpoka, A. O. ve Izevbuwa, O. E., (2019). Methicillin-resistant Staphylococcus aureus (MRSA) and anti-MRSA activities of extracts of some medicinal plants: A brief review, AIMS microbiology, 5 (2), 117.
• Pala, S. A., Wani, A. H. ve Ganai, B. A., (2019). Antimicrobial potential of some wild Macromycetes collected from Kashmir Himalayas, Plant Science Today, 6 (2), 137-146.
• Promphet, N., Prosri, C., Khaeng-raeng, R., Pringsulaka, O. ve Suwannasai, N., (2018). Antioxidant, Antibacterial and Anti-alpha Glucosidase Activities of Coral Mushroom Ramaria spp, SWU Sci. J, 34 (2), 175-191.
• Rodvold, K. A. ve McConeghy, K. W., (2014). Methicillin-resistant Staphylococcus aureus therapy: past, present, and future, Clinical infectious diseases, 58 (suppl_1), S20-S27.
• Sesli, E., Asan, A., Selçuk, F., Abacı Günyar, Ö., Akata, I., Akgül, S., Alkan, S., Allı, H., Aydoğdu, H., Berikten, D., Demirel, K., Demirel, R., Doğan, H. H., Erdoğdu, M., Ergül, C. C., Eroğlu, G., Giray, G., Halikî Uztan, A., Kabaktepe, Ş., Kadaifçiler, D., Kalyoncu, F., Karaltı, İ., Kaşık, G., Kaya, A., Keleş, A., Kırbağ, S., Kıvanç, M., Ocak, İ., Ökten, S., Özkale, E., Öztürk, C., Sevindik, M., Şen, B., Şen, İ., Türkekul, İ., Ulukapı, M., Uzun, Y., Uzun, Y. ve Yoltaş, A. (2020). Türkiye mantarları listesi. İstanbul, Türkiye: ANG Vakfı Yayınları.
• Sevindik, M., Ozdemir, B., Bal, C. ve Selamoglu, Z., (2021). Bioactivity of EtOH and MeOH extracts of Basidiomycetes mushroom (Stereum hirsutum) on atherosclerosis, Archives of Razi Institute, 76 (1), 87.
• Stodůlková, E., Císařová, I., Kolařík, M., Chudíčková, M., Novák, P., Man, P., Kuzma, M., Pavlů, B., Černý, J. ve Flieger, M., (2015). Biologically active metabolites produced by the basidiomycete Quambalaria cyanescens, PLoS One, 10 (2), e0118913.
• URL-1: http://www.indexfungorum.org/names/names.asp (erişim tarihi: 11.07.2024)
• Vallavan, V., Krishnasamy, G., Zin, N. M. ve Abdul Latif, M., (2020). A review on antistaphylococcal secondary metabolites from basidiomycetes, Molecules, 25 (24), 5848.
• Wayne, P., (2011). Clinical and laboratory standards institute. Performance standards for antimicrobial susceptibility testing.
• Zengin, G., Uysal, A., Gunes, E. ve Aktumsek, A., (2014). Survey of phytochemical composition and biological effects of three extracts from a wild plant (Cotoneaster nummularia Fisch. et Mey.): A potential source for functional food ingredients and drug formulations, PLoS One, 9 (11), e113527.