marmara med j Marmara Medical Journal 1019-1941 1309-9469 Marmara University 10.5472/marumj.344797 Clinical Sciences Klinik Tıp Bilimleri Kriyopreservasyon oligoastenoteratozoospermik erkeklerde DNA fragmantasyonunu ve ultrastrüktürel hasarı tetikler Cryopreservation triggers DNA fragmentation and ultrastructural damage in spermatozoa of oligoasthenoteratozoospermic men Turan Pınar Erkanlı Şentürk Gözde Ercan Feriha 05 21 2017 30 2 63 72 02 06 2017 03 29 2017 Copyright © 1988, Marmara Medical Journal 1988 Marmara Medical Journal

Amaç: Oligoastenoteratozoospermi (OAT) erkek infertilitisisebeplerinden biridir. Kriyopreservasyon infertilite yönetimiiçin değerlidir. Bu çalışmanın amacı, OAT’lı hastalardasperm motilitesi, morfolojisi, ultrastrüktürel detaylar ve DNAfragmentasyonu üzerine kriyopreservasyonun etkilerini ortayaçıkarmaktır.Gereç ve Yöntem: Bu gözlemsel çalışmada, gönüllü 20normospermik ve 20 OAT’lı hastadan ejakulatlar toplanmıştır.Ejakulatlar, -196oC sıvı nitrojende saklanmış ve çözdürmeden üçve altı ay sonra analiz edilmiştir. Ejakulatlarda, motilite, morfolojive DNA fragmantasyonu dondurma öncesi ve sonrasındadeğerlendirilmiştir.Bulgular: Her iki grupta da sperm motilitesi ve morfolojisikriyopresvasyondan etkilenmiştir. Her iki grupta da çözdürmedensonra morfolojik değişiklikler anlamlı artmıştır. Ultrastrüktürelincelemeler membran bütünlüğünün değiştiğini ve akrozomalhasarın arttığını göstermiştir. Her iki grupta da, dondurma öncesiile karşılaştırıldığında, çözdürme sonrasında DNA kırıklarıolan hücrelerin arttığı gözlenmiştir. Her iki dondurma-çözmeperiyodunda, kontrol grubu ile kıyaslandığında OAT grubundaDNA kırıkları olan spermatozoa sayısının anlamlı olarak dahayüksek olduğu gözlenmiştir.Sonuç: Kriyopreservasyon, hem normospermik hem de OATgruplarında ultrastrüktürel hasar yapmaktadır ve DNA hasarınıindüklemektedir. Bu hasar OAT hastalarında daha ciddidir.

Objective: Oligoasthenoteratozoospermia (OAT) is one of thecauses of male infertility. Cryopreservation is valuable for themanagement of infertility. This study aimed to reveal the effectsof cryopreservation on sperm motility, morphology, ultrastructuraldetails and DNA fragmentation in patients with OAT.Materials and Methods: In this observational study,ejaculates were collected from 40 male volunteers of whom 20were OAT and 20 were normospermic. The ejaculates were storedin liquid nitrogen at -196oC and analysed following thawing after1 or 3 months. Ejaculates were evaluated in terms of motility,morphology and DNA fragmentation before and after thawing.Results: Sperm motility and morphology were both affectedby cryopreservation in samples from both groups. After thawing,spermatozoa with morphological abnormalities were increasedsignificantly in both groups. Ultrastructural investigations showedalteration in integrity of the membranes and increased acrosomaldefects. Post-thaw investigations revealed prominent increasesin the number of DNA fragmented cells in both groups whencompared to the results before freezing. OAT groups revealed asignificantly higher number of DNA fragmented spermatozoacompared to the normospermic group for both time periods.Conclusion: Cryopreservation produces ultrastructuraldamage and induces DNA fragmentation in both normospermicand OAT groups. The damage is more severe in the OAT group.

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