Objective: In this research, in which the effectiveness of the training given to the cleaning staff who are likely to be carriers forpathogens was investigated, it was also examined whether the socio-demographic characteristics of the participants lead to anydifference in their hygiene practices.Materials and Methods: Training on hygiene was given to the participants by a physician who is specialized in the field of infection.Their knowledge before and after the training was evaluated.Results: It was determined that the rate of desired responses in correct practices regarding hygiene was low, and that there was achange in the scores related to some areas (frequency of going to the dentist, wrong practices in hair hygiene, face towel, hand cleaningmaterial, foot towel practices) after training.Conclusion: In the research, the effect of health training provided to cleaning staff on knowledge and behavior was examined. As aresult of the research, it was observed hat there was a general positive increase related to the hygiene issues in the level of knowledgeand behavior of the cleaning staff.