j res pharm Marmara Pharmaceutical Journal 1309-0801 Marmara University Investigation of the protective effect of Cinnamomum cassia bark extract against H2O2-induced oxidative DNA damage in human peripheral blood lymphocytes and antioxidant activity Investigation of the protective effect of Cinnamomum cassia bark extract against H damage in human peripheral blood lymphocytes and antioxidant activity Sözer Karadağlı Sumru Agrap Borte Lermioğlu Erciyas Ferzan 02 19 2014 18 1 43 48 02 19 2014 Copyright © 1985, Marmara Pharmaceutical Journal 1985 Marmara Pharmaceutical Journal

ABSTRACT: Cinnamon, one of the most widely used spices in the world, has been shown tohave various biological functions including antidiabetic and antitumor activities. Its antidiabeticand antitumor effects were linked with its strong antioxidant activity. In the presentstudy we aimed to investigate the antioxidant activity and possible protective effect of Cinnamomumcassia bark water extract against H2O2-induced oxidative DNA damage.Viability of lymphocytes was determined by Trypan Blue test. For the evaluation of the antioxidantactivity, total phenol and flavonoid contents and 2,2-diphenyl-1-picrylhydrazyl(DPPH) inhibitory activity of the extract were determined. DNA damage was determined byalkaline comet assay in human peripheral blood lymphocytes.Lymphocytes exhibited >86 % survival up till the concentration of 800 μg/ml of the extract.Total phenol and flavonoid contents were calculated as 10.6 g ± 0.001 gallic acid equivalents/100 g dry weight and 2.25±0.004 g quercetin equivalents/100 g dry weight of the extract,respectively. The extract concentration providing 50 % inhibition of DPPH was foundas 76.68 μg/ml.Cinnamomum cassia bark water extract at ≥100 μg/ml concentrations caused significantprotection against H2O2-induced oxidative DNA damage in lymphocytes.Our results support the suggestions that Cinnamomum cassia bark water extract could bebeneficial as a prophylactic agent in prevention of oxidative stress-related diseases.KEYWORDS: Cinnamomum cassia; DPPH; total phenol; total flavonoid; alkaline comet assay.

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