j res pharm

Marmara Pharmaceutical Journal

1309-0801

Marmara University

The effect of betulinic acid on TNBS-induced experimental colitis

Şener

Tarık

Kardaş

Rıza

Şehirli

Ahmet

Ekşioğlu-demiral

Emel

Yüksel

Meral

Çetinel

Şule

Yeğen

Berrak

Şener

Göksel

03 07 2014

17 1 52 59 03 07 2014

1985

Marmara Pharmaceutical Journal

ABSTRACT: In this study we have investigated the possible protective effect of betulinic acid(BA) on colonic inflammation in rats. Colitis was induced in Sprague-Dawley rats of bothsexes by intracolonic administration of 1 ml trinitrobenzene sulphonic acid (TNBS). Colitisinducedrats received orogastrically either betulinic acid (50 mg/kg/day) or vehicle (0.05%DMSO) for 3 days. At the 72nd hour of colitis induction, the rats were decapitated and trunkblood was collected for the measurement of TNF-, IL-1, lactate dehydrogenase (LDH) levelsand total antioxidant capacity (AOC). The distal 8 cm of colon were scored macroscopically,and the degree of oxidant damage was evaluated by malondialdehyde (MDA) andglutathione (GSH) levels, myeloperoxidase activity (MPO), collagen content and by histologicalanalysis. Generation of oxidants was evaluated by tissue luminol and lucigeninchemiluminescences (CL). Colitis caused significant increases in the colonic CL values,macroscopic damage scores, MDA, MPO and collagen levels, along with a significant decreasein tissue GSH level. Similarly, serum TNF-, IL-1, as well as LDH were elevated andAOC was reduced in the vehicle-treated colitis group as compared to control group. On theother hand, betulinic acid treatment reversed all these biochemical indices, as well as histopathologicalalterations induced by TNBS, suggesting that betulinic acid protects the colonictissue via its radical scavenging and antioxidant activities.KEY WORDS: betulinic acid; colitis; oxidative damage; inflammation;trinitrobenzene sulphonic acid

betulinic acid; colitis; oxidative damage; inflammation; trinitrobenzene sulphonic acid

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