j res pharm Marmara Pharmaceutical Journal 1309-0801 Marmara University Health Care Administration Sağlık Kurumları Yönetimi Biosynthesis of copper nanoparticles using aqueous extract of Capparis spinosa fruit and investigation of its antibacterial activity Ebrahımı Katrin Shıravand Sima Mahmoudvand Hossein 12 01 2017 21 4 866 871 06 26 2017 08 02 2017 Copyright © 1985, Marmara Pharmaceutical Journal 1985 Marmara Pharmaceutical Journal

The present study was aimed to use the aqueous extract ofCapparis spinosa to synthesize the copper nanoparticles and alsoevaluated their antibacterial activities again some pathogenicbacterial strains. UV-vis spectroscopy analyses, fouriertransform of infrared (FTIR), scanning electron microscopy(SEM), and energy dispersive X-ray (EDX) were used to identifythe synthesized nanoparticles. The antimicrobial activity ofthe synthesized copper nanoparticles was investigated usingdisk diffusion method and broth microdilution against someGram-positive and Gram-negative bacteria. After adding theextract to the copper sulfate solution, the color of the solutionchanged from light blue to yellowish green. Existence of amaximum peak at the wavelength of 414 nm confirmed theformation of the copper nanoparticles. FTIR spectrum analysisshowed that the factor groups created a coating extract on thesurface of the nanoparticles. Scanning electron microscopydemonstrated the particle size between 17 and 41 nm. Thesefindings showed that Staphylococcus aureus and Bacillus cereusas Gram-positive bacteria were most susceptible to synthesizedcopper nanoparticles in comparison with the Gram-negativebacteria (Klebsiella pneumoniae, and Escherichia coli). Theobtained findings demonstrated that the aqueous extract of C.spinosa acts as a reviver and stabilizer factor. The synthesizedcopper nanoparticles demonstrated activity against both Grampositiveand Gram-negative bacteria.

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