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Melatonin protects against acrylamide- induced oxidative tissue damage in rats

Year 2012, Volume: 16 Issue: 3, 213 - 221, 07.03.2014

Abstract

References

  • Yousef MI, El-Demerdash FM. Acrylamide-induced oxi- dative stress and biochemical perturbations in rats. Toxi- cology 2006; 219: 133-41.
  • Erdreich LS, Friedman MA. Epidemiologic evidence for assessing the carcinogenicity of acrylamide. Regul Toxi- col Pharmacol 2004; 39: 150-7.
  • Tareke E, Heinze TM, Gamboa DA Costa G, Ali S. Acryla- mide formed at physiological temperature as a result of asparagine oxidation. J Agric Food Chem 2009; 57: 9730-3.
  • World Health Organization, Environmental Health Cri- teria 49. Acrylamide. Geneva, Switzerland, 1985.
  • Vanecek J. Cellular mechanisms of melatonin action. Physio Rev 1998; 78: 687-721.
  • Ambriz-Tututi M, Rocha-González HI, Cruz SL, Grana- dos-Soto V. Melatonin: A hormone that modulates pain. Life Sci 2009; 84: 489-98.
  • Tan DX, Chen LD, Poeggeler B, Manchester LC, Reiter RJ. Melatonin: a potent, endogenous hydroxyl radical scavenger. Endocr J 1993; 1: 57-60.
  • Poeggeler B, Saarela S, Reiter RJ, Tan DX, Chen L-D, Manchester LC, Barlow-Walden LR. Melatonin a highly potent endogenous radical scavenger and electron do- nor: new aspects of the oxidation chemistry of this indole accessed in vitro. Ann NY Acad Sci 1994; 738: 419-20.
  • Pieri C, Marra M, Moroni F, Recchioni R, Marcheselli F. Melatonin: a peroxyl radical scavenger more effective than vitamin E. Life Sci 1994; 55: PL271-6.
  • Gilad E, Cuzzocrea S, Zingarelli B, Salzman AL, Szabó C. Melatonin is a scavenger of peroxynitrite. Life Sci 1997; 60: 169-74.
  • Tan DX, Poeggeler B, Reiter RJ. The pineal hormone mela- tonin inhibits DNA adduct formation induced by the chemi- cal carcinogen safrole in vivo. Cancer Lett 1993; 70: 65-71.
  • Reiter RJ, Calvo JR, Karbownik M, Qi W, Tan DX. Mela- tonin and its relation to the immune system and inflam- mation. Ann NY Acad Sci 2000; 917: 376-86.
  • Rosen J, Than NN, Koch D, Poeggeler B, Laatsch H, Hardeland R. Interactions of melatonin and its metabo- lites with the ABTS cation radical: extension of the radi- cal scavenger cascade and formation of a novel class of oxidation products, C2-substituted 3-indolinones. J Pin- eal Res 2006; 41: 374-81.
  • Tan DX, Manchester LC, Terron MP, Flores LJ, Reiter RJ. One molecule, many derivatives: a never-ending inter- action of melatonin with reactive oxygen and nitrogen species? J Pineal Res 2007; 42: 28-42.
  • Manda K, Ueno M, Anzai K. AFMK, a melatonin me- tabolite, attenuates X-ray-induced oxidative damage to DNA, proteins and lipids in mice. J Pineal Res 2007; 42: 386-93.
  • Reiter RJ, Tan DX, Acuna-Castroviejo D, Burkhardt S. Melatonin: mechanisms and actions as an antioxidant. Curr Topics Biophys 2000; 24: 171-83.
  • Reiter RJ, Tan DX, Manchester LC, Qi W. Biochemical re- activity of melatonin with reactive oxygen and nitrogen species. A review of the evidence. Cell Biochem Biophys 2001; 34: 237-56.
  • Talke H, Schubert, GE. Enzymatic urea determination in the blood and serum in the Warburg optical test. Klin Wochen 1965; 43: 174-75.
  • Moss DW, Henderson AR, Kachmar JF. Enzymes. In: Fundamentals of Clinical Chemistry. Tietz NW. Ed., WB Saunders Company, Philadelphia, 1987, pp.372-3.
  • Slot C. Plasma creatinine determination. A new and spe- cific Jaffe reaction method. Scand Clin Lab Invest 1965; 17:381-7.
  • Martinek RG. A rapid ultraviolent spectrophotomeet- ric lactic dehydrogenase assay. Clin Chim Acta 1972; 40:91-9.
  • Beuge JA, Aust SD. Microsomal lipid peroxidation. Methods Enzymol 1978; 52: 302-11.
  • Beutler E. Glutathione in red blood cell metabolism. In A Manuel of Biochemical Methods, Grune&Strattonb, NewYork, 1975; pp.114-22.
  • Hillegass LM, Griswold DE, Brickson B, Albrightson- Winslow C. Assessment of myeloperoxidase activity in whole rat kidney. J Pharmacol Methods 1990; 24: 285-95.
  • Lopez De Leon A, Rojkind M. A simple micromethod for collagen and total protein determination in formalin- fixed parraffin-embedded sections. J Histochem Cyto- chem 1985; 33: 737-43.
  • Takei H, Araki A, Watanabe H, Ichinose A, Sendo F. Rapid killing of human neutrophils by the potent activa- tor phorbol 12-myristate 13-acetate (PMA) accompanied by changes different from typical apoptosis or necrosis. J Leukocyte Biol 1996; 59: 229-40.
  • Rayburn JR, Friedman M. L-Cysteine, N-Acetyl-l- cysteine, and Glutathione Protect Xenopus laevis Em- bryos against Acrylamide-Induced Malformations and Mortality in the Frog Embryo Teratogenesis Assay. J Ag- ric Food Chem. 2010; 58: 11172-8.
  • Kopp EB, Ghosh S. NF-kappa B and rel proteins in innate immunity. Adv Immunol 1995; 58: 1-27.
  • Park YC, Rimbach G, Saliou C, Valacchi G, Packer L. Ac- tivity of monomeric, dimeric, and trimeric flavonoids on NO production, TNF-alpha secretion, and NF-kappaB- dependent gene expression in RAW 264.7 macrophages. FEBS Lett 2000; 465: 93-7.
  • Rezende-Neto JB, Moore EE, Melo De Andrade MV, Teixeira MM, Lisboa FA, Arantes RM, de Souza DG, da Cunha-Melo JR. Systemic inflammatory response secondary to abdominal compartment syndrome: Stage for multiple organ failure. J Trauma 2002; 53: 1121-8.
  • Radons J, Heller B, Bürkle A, Hartmann B, Rodriguez ML, Kröncke KD, Burkart V, Kolb H. Nitric oxide toxic- ity in islet cells involves poly(ADP-ribose) polymerase activation and concomitant NAD+ depletion. Biochem Biophys Res Commun 1994; 30: 1270-7.
  • Pacher P, Liaudet L, Mabley JG, Komjáti K, Szabó C. Pharmacologic inhibition of poly (adenosine diphos- phateribose) polymerase may represent a novel thera- peutic approach in chronic heart failure. J Am Coll Car- diol 2002; 40: 1006-16.
  • Dizdaroglu M. Oxidative damage to DNA in mamma- lian chromatin. Mutat Res 1992; 275: 331-42.
  • Shigenaga MK, Hagen TM, Ames BN. Oxidative damage and mitochondrial decay in aging. Proc Natl Acad Sci USA 1994; 91: 10771-8.
  • Stark G. Functional Consequences of Oxidative Mem- brane Damage. J Membrane Biol 2005; 205: 1-16.
  • Yamamoto Y. Oxidation of biological membranes and its in- hibition. Free radical chain oxidation of erythrocytes ghost membranes by oxygen. Biochem Acta 1985; 819: 29-36.
  • Jaworek J, Leja-Szpak A, Nawrot-Porabka K, Bonior J, Szklarczyk J, Kot M, Konturek SJ, Tomaszewska R, Pawlik WW. Effect of neonatal endotoxemia on the pancreas of adult rats. J Physiol Pharmacol 2008; 59: 87-102.
  • El-Sokkary GH, Reiter RJ, Tan DX, Kim SJ, Cabrera J. Inhibitory effect of melatonin on products of lipid per- oxidation resulting from chronic ethanol administration. Alcohol and Alcoholism 1999; 34: 842-50.
  • Kurebayashi H, Ohno Y. Metabolism of acrylamide to glycidamide and their cytotoxicity in isolated rat hepato- cytes: protective effects of GSH precursors. Arch Toxicol 2006; 80: 820-8.
  • Oliveira NG, Pingarilho M, Martins C, Fernandes AS, Vaz S, Martins V, Rueff J, Gaspar JF. Cytotoxicity and chromosomal aberrations induced by acrylamide in V79 cells: role of glutathione modulators. Mutat Res 2009; 676: 87-92.
  • Schulze-Osthoff K, Los M, Baeuerle PA. Redox signal- ling by transcription factors NF-kappa B and AP-1 in lymphocytes. Biochem Pharmacol 1995; 50: 735-41.
  • Klaunig JE, Kamendulis LM. Mechanisms of acrylamide induced rodent carcinogenesis. In: Chemistry and Safety of Acrylamide in Food; Friedman M, Mottram M Eds., Springer, New York, 2005; pp.49-62.
  • Atkuri KR, Mantovani JJ, Herzenberg LA, Herzenberg LA. N-Acetylcysteine-a safe antidote for cysteine/glu- tathione deficiency. Curr Opin Pharmacol 2007; 7: 355-9.
  • Friedman M. Improvement in the safety of foods by SH- containing amino acids and peptides. A review. J Agric Food Chem 1994; 42: 3-20.
  • Okatani Y, Wakatsuki A, Shinohara K, Kaneda C, Fu- kaya T. Melatonin stimulates glutathione peroxidase ac- tivity in human chorion. J Pineal Res 2001; 30: 199-205.
  • Albarran MT, Lopez-Burillo S, Pablos MI, Reiter RJ, Agapito MT. Endogenous rythms of melatonin, total antioxidant status and superoxide dismutase activity in several tissues of chick and their inhibition by light. J Pineal Res 2001; 30: 227-33.
  • Kettle AJ, Winterbourn CC. Myeloperoxidase: a key reg- ulator of neutrophil oxidant production. Redox Report 1997; 3: 3-15.
  • Sener G, Sehirli O, Yegen BC, Cetinel S, Gedik N, Sakar- can A. Melatonin attenuates ifosfamide-induced Fanconi syndrome in rats. J Pineal Res 2004; 37: 17-25.
  • Sener G, Sert G, Sehirli AO, Arbak S, Gedik N, Ayanoğlu- Dülger G. Melatonin protects against pressure ulcer-in- duced oxidative injury of the skin and remote organs in rats. J Pineal Res 2006; 40: 280-7.

Melatonin protects against acrylamideinduced oxidative tissue damage in rats

Year 2012, Volume: 16 Issue: 3, 213 - 221, 07.03.2014

Abstract

ABSTRACT: Acrylamide (ACR), is a widely used industrial chemical which induces oxidative
stress in the body. In this study we aimed to investigate the possible protective effect of
melatonin (MEL), as an antioxidant agent, against experimental ACR toxicity in the liver and
kidney of the rats. Wistar albino rats of either sex 200-250 g were divided into four groups
each consisting of 6 animals. Rats received for 10 days; 1) 0.9% NaCl i.p.; control (C) group;
2) MEL in a dose of 10 mg/kg i.p., 3) ACR in a dose of 40 mg/kg i.p. (dissolved in 0.9% NaCl)
(ACR group) and 4) MEL (in a dose of 10 mg/kg i.p.) and ACR (in a dose of 40 mg/kg i.p.).
After decapitation, liver and kidney tissues were excised. Malondialdehyde (MDA), glutathione
(GSH) levels, collagen contents and myeloperoxidase activity (MPO) were determined
in the tissues, while enzyme activities and cytokine levels were assayed in blood
samples. In the ACR treated group, GSH levels decreased significantly while the MDA levels,
MPO activity and collagen content increased in the tissues suggesting oxidative organ
damage. In the MEL treated ACR group, all of these oxidant responses were reversed significantly.
Serum enzyme activities, cytokine levels and leukocyte apoptosis which increased
significantly following ACR administration, decreased with MEL treatment. The results demonstrate
the role of oxidative mechanisms in ACR-induced tissue damage, and melatonin, by
its antioxidant properties, ameliorates oxidative organ injury due to acrylamide toxicity.
KEY WORDS: Acrylamide, melatonin, lipid peroxidation, glutathione, myeloperoxidase

References

  • Yousef MI, El-Demerdash FM. Acrylamide-induced oxi- dative stress and biochemical perturbations in rats. Toxi- cology 2006; 219: 133-41.
  • Erdreich LS, Friedman MA. Epidemiologic evidence for assessing the carcinogenicity of acrylamide. Regul Toxi- col Pharmacol 2004; 39: 150-7.
  • Tareke E, Heinze TM, Gamboa DA Costa G, Ali S. Acryla- mide formed at physiological temperature as a result of asparagine oxidation. J Agric Food Chem 2009; 57: 9730-3.
  • World Health Organization, Environmental Health Cri- teria 49. Acrylamide. Geneva, Switzerland, 1985.
  • Vanecek J. Cellular mechanisms of melatonin action. Physio Rev 1998; 78: 687-721.
  • Ambriz-Tututi M, Rocha-González HI, Cruz SL, Grana- dos-Soto V. Melatonin: A hormone that modulates pain. Life Sci 2009; 84: 489-98.
  • Tan DX, Chen LD, Poeggeler B, Manchester LC, Reiter RJ. Melatonin: a potent, endogenous hydroxyl radical scavenger. Endocr J 1993; 1: 57-60.
  • Poeggeler B, Saarela S, Reiter RJ, Tan DX, Chen L-D, Manchester LC, Barlow-Walden LR. Melatonin a highly potent endogenous radical scavenger and electron do- nor: new aspects of the oxidation chemistry of this indole accessed in vitro. Ann NY Acad Sci 1994; 738: 419-20.
  • Pieri C, Marra M, Moroni F, Recchioni R, Marcheselli F. Melatonin: a peroxyl radical scavenger more effective than vitamin E. Life Sci 1994; 55: PL271-6.
  • Gilad E, Cuzzocrea S, Zingarelli B, Salzman AL, Szabó C. Melatonin is a scavenger of peroxynitrite. Life Sci 1997; 60: 169-74.
  • Tan DX, Poeggeler B, Reiter RJ. The pineal hormone mela- tonin inhibits DNA adduct formation induced by the chemi- cal carcinogen safrole in vivo. Cancer Lett 1993; 70: 65-71.
  • Reiter RJ, Calvo JR, Karbownik M, Qi W, Tan DX. Mela- tonin and its relation to the immune system and inflam- mation. Ann NY Acad Sci 2000; 917: 376-86.
  • Rosen J, Than NN, Koch D, Poeggeler B, Laatsch H, Hardeland R. Interactions of melatonin and its metabo- lites with the ABTS cation radical: extension of the radi- cal scavenger cascade and formation of a novel class of oxidation products, C2-substituted 3-indolinones. J Pin- eal Res 2006; 41: 374-81.
  • Tan DX, Manchester LC, Terron MP, Flores LJ, Reiter RJ. One molecule, many derivatives: a never-ending inter- action of melatonin with reactive oxygen and nitrogen species? J Pineal Res 2007; 42: 28-42.
  • Manda K, Ueno M, Anzai K. AFMK, a melatonin me- tabolite, attenuates X-ray-induced oxidative damage to DNA, proteins and lipids in mice. J Pineal Res 2007; 42: 386-93.
  • Reiter RJ, Tan DX, Acuna-Castroviejo D, Burkhardt S. Melatonin: mechanisms and actions as an antioxidant. Curr Topics Biophys 2000; 24: 171-83.
  • Reiter RJ, Tan DX, Manchester LC, Qi W. Biochemical re- activity of melatonin with reactive oxygen and nitrogen species. A review of the evidence. Cell Biochem Biophys 2001; 34: 237-56.
  • Talke H, Schubert, GE. Enzymatic urea determination in the blood and serum in the Warburg optical test. Klin Wochen 1965; 43: 174-75.
  • Moss DW, Henderson AR, Kachmar JF. Enzymes. In: Fundamentals of Clinical Chemistry. Tietz NW. Ed., WB Saunders Company, Philadelphia, 1987, pp.372-3.
  • Slot C. Plasma creatinine determination. A new and spe- cific Jaffe reaction method. Scand Clin Lab Invest 1965; 17:381-7.
  • Martinek RG. A rapid ultraviolent spectrophotomeet- ric lactic dehydrogenase assay. Clin Chim Acta 1972; 40:91-9.
  • Beuge JA, Aust SD. Microsomal lipid peroxidation. Methods Enzymol 1978; 52: 302-11.
  • Beutler E. Glutathione in red blood cell metabolism. In A Manuel of Biochemical Methods, Grune&Strattonb, NewYork, 1975; pp.114-22.
  • Hillegass LM, Griswold DE, Brickson B, Albrightson- Winslow C. Assessment of myeloperoxidase activity in whole rat kidney. J Pharmacol Methods 1990; 24: 285-95.
  • Lopez De Leon A, Rojkind M. A simple micromethod for collagen and total protein determination in formalin- fixed parraffin-embedded sections. J Histochem Cyto- chem 1985; 33: 737-43.
  • Takei H, Araki A, Watanabe H, Ichinose A, Sendo F. Rapid killing of human neutrophils by the potent activa- tor phorbol 12-myristate 13-acetate (PMA) accompanied by changes different from typical apoptosis or necrosis. J Leukocyte Biol 1996; 59: 229-40.
  • Rayburn JR, Friedman M. L-Cysteine, N-Acetyl-l- cysteine, and Glutathione Protect Xenopus laevis Em- bryos against Acrylamide-Induced Malformations and Mortality in the Frog Embryo Teratogenesis Assay. J Ag- ric Food Chem. 2010; 58: 11172-8.
  • Kopp EB, Ghosh S. NF-kappa B and rel proteins in innate immunity. Adv Immunol 1995; 58: 1-27.
  • Park YC, Rimbach G, Saliou C, Valacchi G, Packer L. Ac- tivity of monomeric, dimeric, and trimeric flavonoids on NO production, TNF-alpha secretion, and NF-kappaB- dependent gene expression in RAW 264.7 macrophages. FEBS Lett 2000; 465: 93-7.
  • Rezende-Neto JB, Moore EE, Melo De Andrade MV, Teixeira MM, Lisboa FA, Arantes RM, de Souza DG, da Cunha-Melo JR. Systemic inflammatory response secondary to abdominal compartment syndrome: Stage for multiple organ failure. J Trauma 2002; 53: 1121-8.
  • Radons J, Heller B, Bürkle A, Hartmann B, Rodriguez ML, Kröncke KD, Burkart V, Kolb H. Nitric oxide toxic- ity in islet cells involves poly(ADP-ribose) polymerase activation and concomitant NAD+ depletion. Biochem Biophys Res Commun 1994; 30: 1270-7.
  • Pacher P, Liaudet L, Mabley JG, Komjáti K, Szabó C. Pharmacologic inhibition of poly (adenosine diphos- phateribose) polymerase may represent a novel thera- peutic approach in chronic heart failure. J Am Coll Car- diol 2002; 40: 1006-16.
  • Dizdaroglu M. Oxidative damage to DNA in mamma- lian chromatin. Mutat Res 1992; 275: 331-42.
  • Shigenaga MK, Hagen TM, Ames BN. Oxidative damage and mitochondrial decay in aging. Proc Natl Acad Sci USA 1994; 91: 10771-8.
  • Stark G. Functional Consequences of Oxidative Mem- brane Damage. J Membrane Biol 2005; 205: 1-16.
  • Yamamoto Y. Oxidation of biological membranes and its in- hibition. Free radical chain oxidation of erythrocytes ghost membranes by oxygen. Biochem Acta 1985; 819: 29-36.
  • Jaworek J, Leja-Szpak A, Nawrot-Porabka K, Bonior J, Szklarczyk J, Kot M, Konturek SJ, Tomaszewska R, Pawlik WW. Effect of neonatal endotoxemia on the pancreas of adult rats. J Physiol Pharmacol 2008; 59: 87-102.
  • El-Sokkary GH, Reiter RJ, Tan DX, Kim SJ, Cabrera J. Inhibitory effect of melatonin on products of lipid per- oxidation resulting from chronic ethanol administration. Alcohol and Alcoholism 1999; 34: 842-50.
  • Kurebayashi H, Ohno Y. Metabolism of acrylamide to glycidamide and their cytotoxicity in isolated rat hepato- cytes: protective effects of GSH precursors. Arch Toxicol 2006; 80: 820-8.
  • Oliveira NG, Pingarilho M, Martins C, Fernandes AS, Vaz S, Martins V, Rueff J, Gaspar JF. Cytotoxicity and chromosomal aberrations induced by acrylamide in V79 cells: role of glutathione modulators. Mutat Res 2009; 676: 87-92.
  • Schulze-Osthoff K, Los M, Baeuerle PA. Redox signal- ling by transcription factors NF-kappa B and AP-1 in lymphocytes. Biochem Pharmacol 1995; 50: 735-41.
  • Klaunig JE, Kamendulis LM. Mechanisms of acrylamide induced rodent carcinogenesis. In: Chemistry and Safety of Acrylamide in Food; Friedman M, Mottram M Eds., Springer, New York, 2005; pp.49-62.
  • Atkuri KR, Mantovani JJ, Herzenberg LA, Herzenberg LA. N-Acetylcysteine-a safe antidote for cysteine/glu- tathione deficiency. Curr Opin Pharmacol 2007; 7: 355-9.
  • Friedman M. Improvement in the safety of foods by SH- containing amino acids and peptides. A review. J Agric Food Chem 1994; 42: 3-20.
  • Okatani Y, Wakatsuki A, Shinohara K, Kaneda C, Fu- kaya T. Melatonin stimulates glutathione peroxidase ac- tivity in human chorion. J Pineal Res 2001; 30: 199-205.
  • Albarran MT, Lopez-Burillo S, Pablos MI, Reiter RJ, Agapito MT. Endogenous rythms of melatonin, total antioxidant status and superoxide dismutase activity in several tissues of chick and their inhibition by light. J Pineal Res 2001; 30: 227-33.
  • Kettle AJ, Winterbourn CC. Myeloperoxidase: a key reg- ulator of neutrophil oxidant production. Redox Report 1997; 3: 3-15.
  • Sener G, Sehirli O, Yegen BC, Cetinel S, Gedik N, Sakar- can A. Melatonin attenuates ifosfamide-induced Fanconi syndrome in rats. J Pineal Res 2004; 37: 17-25.
  • Sener G, Sert G, Sehirli AO, Arbak S, Gedik N, Ayanoğlu- Dülger G. Melatonin protects against pressure ulcer-in- duced oxidative injury of the skin and remote organs in rats. J Pineal Res 2006; 40: 280-7.
There are 49 citations in total.

Details

Primary Language English
Journal Section Articles
Authors

Ayfer Tozan-beceren This is me

Ahmet Şehirli This is me

Emel Eksioglu-demiralp This is me

Göksel Şener This is me

Gülden Omurtag This is me

Publication Date March 7, 2014
Published in Issue Year 2012 Volume: 16 Issue: 3

Cite

APA Tozan-beceren, A., Şehirli, A., Eksioglu-demiralp, E., Şener, G., et al. (2014). Melatonin protects against acrylamideinduced oxidative tissue damage in rats. Marmara Pharmaceutical Journal, 16(3), 213-221. https://doi.org/10.12991/mpj.89574
AMA Tozan-beceren A, Şehirli A, Eksioglu-demiralp E, Şener G, Omurtag G. Melatonin protects against acrylamideinduced oxidative tissue damage in rats. Marmara Pharm J. March 2014;16(3):213-221. doi:10.12991/mpj.89574
Chicago Tozan-beceren, Ayfer, Ahmet Şehirli, Emel Eksioglu-demiralp, Göksel Şener, and Gülden Omurtag. “Melatonin Protects Against Acrylamideinduced Oxidative Tissue Damage in Rats”. Marmara Pharmaceutical Journal 16, no. 3 (March 2014): 213-21. https://doi.org/10.12991/mpj.89574.
EndNote Tozan-beceren A, Şehirli A, Eksioglu-demiralp E, Şener G, Omurtag G (March 1, 2014) Melatonin protects against acrylamideinduced oxidative tissue damage in rats. Marmara Pharmaceutical Journal 16 3 213–221.
IEEE A. Tozan-beceren, A. Şehirli, E. Eksioglu-demiralp, G. Şener, and G. Omurtag, “Melatonin protects against acrylamideinduced oxidative tissue damage in rats”, Marmara Pharm J, vol. 16, no. 3, pp. 213–221, 2014, doi: 10.12991/mpj.89574.
ISNAD Tozan-beceren, Ayfer et al. “Melatonin Protects Against Acrylamideinduced Oxidative Tissue Damage in Rats”. Marmara Pharmaceutical Journal 16/3 (March 2014), 213-221. https://doi.org/10.12991/mpj.89574.
JAMA Tozan-beceren A, Şehirli A, Eksioglu-demiralp E, Şener G, Omurtag G. Melatonin protects against acrylamideinduced oxidative tissue damage in rats. Marmara Pharm J. 2014;16:213–221.
MLA Tozan-beceren, Ayfer et al. “Melatonin Protects Against Acrylamideinduced Oxidative Tissue Damage in Rats”. Marmara Pharmaceutical Journal, vol. 16, no. 3, 2014, pp. 213-21, doi:10.12991/mpj.89574.
Vancouver Tozan-beceren A, Şehirli A, Eksioglu-demiralp E, Şener G, Omurtag G. Melatonin protects against acrylamideinduced oxidative tissue damage in rats. Marmara Pharm J. 2014;16(3):213-21.