Glycyl-L-Histidyl-L-Liysine-Cu(2+) loaded liposome formulations

Year 2010, Volume: 14 Issue: 2, 91 - 97, 03.03.2014

Setenay Erdem Murat Türkoğlu

Abstract

ABSTRACT: Enhancement of collagen synthesis by glycyl-l-histidyl-l-lysine-Cu2+ (GHK-Cu)
derivatives is well known. The different activities of GHK-Cu would be of interest for cosmetic
applications. Liposomes provide many benefits as topical drug delivery systems.
Structure of double layer and lipid composition of liposomes keep the active substance
longer in skin and provide regularly release to the deeper skin layers. Our aim in this study
was to prepare GHK-Cu loaded liposomes and characterize them to use in a cosmetic formulation.
UV spectrophotometric method was used to detect the GHK-Cu in aqueous medium
and FTIR spectrums were taken to determine the absorption bands. In stability studies, it
was observed that aqueous solutions of GHK-Cu samples maintained their stability at 4oC
for 4 months and the FTIR absorption bands of powdered GHK-Cu did not change when
stored under the same stability conditions with aqueous samples. Different liposome formulations
were prepared by lipid film hydration technique using different kinds of phospholipids
(dipalmitoylphosphatidylcholine (DPPC-5911), Epicuron 100H, and Epicuron 200SH).
The particle size and shape of liposomes were determined using microscope, SEM, and laser
diffraction method. The average particle size was found to be 13

Keywords

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Glycyl-L-Histidyl-L-Liysine-Cu(2+) loaded liposome formulations

Abstract

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