Antioxidant, antimicrobial and anticarcinogenic activities of Sambucus ebulus L. flowers, fruits and leaves

Year 2014, Volume: 18 Issue: 1, 22 - 25, 15.02.2014

Zehra Meriç Leyla Bitiş , Seher Birteksöz Tan Suna Özbaş Turan , Jülide Akbuga

Abstract

ABSTRACT: The aim of this study was to evaluate the antioxidant, antimicrobial and anticarsinogenic
activities of methanol extract obtained from flowers, fruits and leaves of Sambucus
ebulus L. Free radical scavenging activities and total phenolic contents of the species
were assayed by DPPH method and Folin–Ciocalteu method, respectively. The antimicrobial
activity of the extracts were tested by the micro-broth dilution method against seven microbial
species. L929 (ATCC, CCL-1) mouce fibroblast cell line was used for the determiation of
in vitro citotoxicity activity and HeLa (ATCC, CCl-2) human cervics adenocarcinoma cell line
was used for the determination of anticarcinogenic and growth inhibition activity. Leaves of
Sambucus ebulus L showed the strongest antioxidant activity and also leaves of plant has
highest phenolic content, Sambucus ebulus L. exracts were found to have no significant
activity against any strains of bacteria. Only Sambucus ebulus fruit extract has shown moderate
effect against Candida albicans. In 10μg/mL concentration the highest anticarcinogenic
activity were found in leaves extract, in addition that none of the extracts were not found
cytotoxic.
KEY WORDS: Sambucus ebulus, antioxidant, antimicrobial, anticarsinogenic, cytotoxic

Keywords

Sambucus ebulus, antioxidant, antimicrobial, anticarsinogenic, cytotoxic

Antioxidant, antimicrobial and anticarcinogenic activities of Sambucus ebulus L. flowers, fruits and leaves

Abstract

Bu çalışmanın amacı Türkiye’de yetişen Sambucus ebulus L. bitkisinin çiçek, meyve ve yapraklarından hazırlanan metanol ekstrelerinin antioksidan, antimikrobiyal ve antikarsinojenik aktivitelerini değerlendirmektir. Ekstrelerin serbest radikal süpürücü aktivite tayinleri DPPH metodu ile, toplam fenolik madde miktar tayini ise Folin–Ciocalteu metoduyla, antimikrobiyal aktivitesi 7 mikroorganizma türüne karşı mikro dilüsyon yöntemiyle, antikarsinojenik aktivite tayini ise L929 (ATCC, CCL-1) fare fibroblast hücre hattı ve HeLa (ATCC, CCl-2) kanser hücre hattı kullanılarak MTT yöntemiyle sitotoksik etkinlikleri araştırmak üzere test edildi. DPPH radikal süpürücü aktivite tayininde en yüksek aktiviteyi yaprak ekstresi gösterdi ayrıca toplam fenolik madde miktarı da en yüksek oranda bu ekstrede bulundu. Antimikrobiyal aktivite tayininde sadece meyve ekstresi Candida albicans’a karşı orta derecede etki göstermiştir. Antikarsinojenik aktivite test sonuçlarına göre ise yaprak ekstresi en yüksek değeri göstermiş, hiçbir ekstre sitotoksik bulunmamıştır.

Keywords

Sambucus ebulus, antioksidan, antimikrobiyal, antikarsinojenik, sitotoksik1: Flowers, 2: Fruits, 3: LeavesFIGURE 1. Cell viability and growth inhibition

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