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Investigation of the protective effect of Cinnamomum cassia bark extract against H2O2-induced oxidative DNA damage in human peripheral blood lymphocytes and antioxidant activity

Year 2014, Volume: 18 Issue: 1, 43 - 48, 19.02.2014

Abstract

ABSTRACT: Cinnamon, one of the most widely used spices in the world, has been shown to
have various biological functions including antidiabetic and antitumor activities. Its antidiabetic
and antitumor effects were linked with its strong antioxidant activity. In the present
study we aimed to investigate the antioxidant activity and possible protective effect of Cinnamomum
cassia bark water extract against H2O2-induced oxidative DNA damage.
Viability of lymphocytes was determined by Trypan Blue test. For the evaluation of the antioxidant
activity, total phenol and flavonoid contents and 2,2-diphenyl-1-picrylhydrazyl
(DPPH) inhibitory activity of the extract were determined. DNA damage was determined by
alkaline comet assay in human peripheral blood lymphocytes.
Lymphocytes exhibited >86 % survival up till the concentration of 800 μg/ml of the extract.
Total phenol and flavonoid contents were calculated as 10.6 g ± 0.001 gallic acid equivalents/
100 g dry weight and 2.25±0.004 g quercetin equivalents/100 g dry weight of the extract,
respectively. The extract concentration providing 50 % inhibition of DPPH was found
as 76.68 μg/ml.
Cinnamomum cassia bark water extract at ≥100 μg/ml concentrations caused significant
protection against H2O2-induced oxidative DNA damage in lymphocytes.
Our results support the suggestions that Cinnamomum cassia bark water extract could be
beneficial as a prophylactic agent in prevention of oxidative stress-related diseases.
KEYWORDS: Cinnamomum cassia; DPPH; total phenol; total flavonoid; alkaline comet assay.

References

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  • Diplock AT, Charleux J-L, Crozier-Willi G, Kok FJ, RiceEvans C, Roberfroid M, Stah W, Viiia-Ribes J. Functional food science and defence against reactive oxidative species. Br J Nutr 1998; 80(Suppl 1):77-112.
  • Valko M, Izakovic M, Mazur M, Rhodes C. J, Telser J. Role of oxygen radicals in DNA damage and cancer incidence. Mol Cell Biochem 2004; 266:37-56.
  • Valko M, Leibfritz D, Moncol J, Cronin M.T, Mazur M, Telser J. Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol 2007; 39:44-84.
  • Atanassova M, Georgieva S, Ivancheva K. Total phenolic and Total Flavonoid Contents, antioxidant capacity and biological components in medicinal herbs, J Univ Chem Tech and Metal 2011; 46:81-8.
  • Kaur P, Walia A, Kumar S, Kaur S. Antigenotoxic Activity of Polyphenolic Rich Extracts from Aegle marmelos (L.) Correa in Human Blood Lymphocytes and E.coli PQ Rec Nat Prod 2009; 3:68-75.
  • Totter JR. Spontaneous cancer and its possible relationship to oxygen metabolism. Proc Natl Acad Sci USA 1980; 77:1763-7.
  • Ames BN, Shigenaga MK, Hagen TM. Oxidants, antioxidants and the degenerative diseases of aging. Proc Natl Acad Sci USA 1993; 90:7915-22.
  • Roy N, Laskar RA, Kumari D, Ghosh T, Begum,NA. A detailed study on the antioxidant activity of the stem bark of Dalbergia sissoo Roxb., an Indian Medicinal Plant. Food Chem, 2011; 126:1115-21.
  • Koppikar SJ, Choudhari AS, Suryavanshi SA, Kumari S, Chattopadhyay S, Kaul-Ghanekar R. Aqueous Cinnamon Extract (ACE-c) from the bark of Cinnamomum cassia causes apoptosis in human cervical cancer cell line (SiHa) through loss of mitochondrial membrane potential. BMC Cancer 2010; 18:210-22.
  • Shan B, Cai YZ, Sun M, Corke H. Antioxidant capacity of 26 spice extracts and characterization of their phenolic constituents. J Agric Food Chem 2005; 5;53:7749-59.
  • Rubio L, Motilva MJ, Romero MP. Recent advances in biologically active compounds in herbs and spices: a review of the most effective antioxidant and anti-inflammatory active principles. Crit Rev Food Sci Nutr 2013; 53:943-53.
  • Craig WJ. Phytochemicals: guardians of our health. J Am Diet Assoc 1997; 97(10 Suppl 2):199-204.
  • Liu RH. Potential synergy of phytochemicals in cancer prevention: mechanism of action. J Nutr 2004; 134(12 Suppl):3479-85.
  • Kaefer CM, Milner JA. The role of herbs and spices in cancer prevention. J Nutr Biochem 2008;19:19347-61.
  • Gruenwald J, Freder J, Armbruester N. Cinnamon and Health. Crit Rev Food Sci Nutr 2010; 50:822-34.
  • Kwon HK, Jeon WK, Hwang JS, Lee CG, So JS, Park JA, Ko BS, Im SH. Cinnamon extract suppresses tumor progression by modulating angiogenesis and the effector function of CD8+ T cells. Cancer Lett 2009; 278:174-82.
  • Khan A, Safdar M, Ali Khan MM, Khattak KN, Anderson RA. Cinnamon improves glucose and lipids of people with Type 2 diabetes. Diabetes Care 2003; 26:3215–8.
  • Anderson RA. Chromium and polyphenols from cinnamon improve insulin sensitivity. Proceed Nutr Soc 2008; 67:48–53.
  • Schoene NW, Kelly MA, Polansky MM, Anderson RA. Watersoluble polymeric polyphenols from cinnamon inhibit proliferation and alter cell cycle distribution patterns of hematologic tumor cell lines. Cancer Lett 2005; 230:134-40.
  • Kamei T, Kumano H, Iwata K, Nariai Y, Matsumoto T. The effect of a traditional Chinese prescription for a case of lung carcinoma. J Alternat Complemen Med 2000; 6:557-9.
  • Singh R, Koppikar SJ, Paul P, Gilda S, Paradkar AR, Kaul-Ghanekar R. Comparative analysis of cytotoxic effect of aqueous cinnamon extract from Cinnamomum zeylanicum bark with commercial cinnamaldehyde on various cell lines. Phar Bio 2009; 47:1174-9.
  • Cao H, Urban JF Jr, Anderson RA. Cinnamon polyphenol extract affects immune responses by regulating antiand proinflammatory and glucose transporter gene expression in mouse macrophages. J Nutr 2008; 138:833-40.
  • Wondrak GT, Villeneuve NF, Lamore SD, Bause AS, Jiang Tao Z, Donna D. The Cinnamon-Derived Dietary Factor Cinnamic Aldehyde Activates the Nrf2-Dependent Antioxidant Response in Human Epithelial Colon Cells. Molecules 2010; 15:3338–55.
  • Qin B, Dawson HD, Schoene NW, Polansky MM, Anderson RA. Cinnamon polyphenols regulate multiple metabolic pathways involved in insulin signaling and intestinal lipoprotein metabolism of small intestinal enterocytes. Nutrition 2012; 28:1172-79.
  • Doyle A, Griffiths JB, Newell DG. Cell and Tissue Culture: Laboratory Procedures, John Wiley & Sons,Inc., Chichester, England 1995.
  • Singleton VL, Rossi JA. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am J Enol Vitic 1965; 16:144–53.
  • Chang C, Yang M, Wen H. Estimation of total flavonoid content in propolis by two complementary colorimetric methods, J Food Drug Anal 2002; 10:178-82.
  • Yang CH, Li RX, Chuang LY. Antioxidant activity of various parts of Cinnamomum cassia extracted with different extraction methods. Molecules 2012; 17:7294-304.
  • Kamleshiya P, Meshram VG, Ansari AH. Comparative evaluation of antioxidant and free-radical scavenging activity of aqueous and methanolic spice extracts. Int J Life Sci Pharm Res 2012; 2:118-25.
  • Jothi SL, Zuraini Z, Sasidharan S. Phytochemical screening DPPH free radical scavenging and xanthine oxidase inhibitory activities of Cassia fistula seeds extract. J Med Plant Res 2011; 5:1941-7.
  • Collins AR, Dobson VL, Dusinska M, Kenned G, Stetina R. The Comet Assay: what can it really tell us? Mutat Res 1997; 375:183–93.
  • Singh NP, Mc Coy MT, Tice RP, Schneider EL. A simple technique for quantitation of low levels of DNA damage in individual cells. Exp Cell Res 1988; 175:184-91.
  • Wong VWC, Szeto YT, Collins AR, Benzie IFF. The Comet assay: a biomonitoring tool for nutraceutical research. Curr Topics in Nutr Res 2005; 3:1–14.
  • Kwon HK, Hwang JS, So JS, Lee CG, Sahoo A, Ryu JH, Jeon WK, Ko BS, Im CR, Lee SH, Park ZY, Im SH. Cinnamon extract induces tumor cell death through inhibition of NFκB and AP1. BMC Cancer 2010; 10:392-401.
  • Jang HD, Chang KS, Huang YS, Hsu CL, Lee SH, Su MS. Principal phenolic phytochemicals and antioxidant activities of three Chinese medicinal plants. Food Chem 2007; 103:749–56.
  • Diplock AT. Antioxidant nutrients and disease prevention: an overview. Am J Clin Nutr 1991; 53:189-93.

Investigation of the protective effect of Cinnamomum cassia bark extract against H damage in human peripheral blood lymphocytes and antioxidant activity

Year 2014, Volume: 18 Issue: 1, 43 - 48, 19.02.2014

Abstract

References

  • Halliwell B. Free radicals, antioxidants, and human disease: curiosity, cause, or consequence? Lancet 1994; 344:721-4.
  • Diplock AT, Charleux J-L, Crozier-Willi G, Kok FJ, RiceEvans C, Roberfroid M, Stah W, Viiia-Ribes J. Functional food science and defence against reactive oxidative species. Br J Nutr 1998; 80(Suppl 1):77-112.
  • Valko M, Izakovic M, Mazur M, Rhodes C. J, Telser J. Role of oxygen radicals in DNA damage and cancer incidence. Mol Cell Biochem 2004; 266:37-56.
  • Valko M, Leibfritz D, Moncol J, Cronin M.T, Mazur M, Telser J. Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol 2007; 39:44-84.
  • Atanassova M, Georgieva S, Ivancheva K. Total phenolic and Total Flavonoid Contents, antioxidant capacity and biological components in medicinal herbs, J Univ Chem Tech and Metal 2011; 46:81-8.
  • Kaur P, Walia A, Kumar S, Kaur S. Antigenotoxic Activity of Polyphenolic Rich Extracts from Aegle marmelos (L.) Correa in Human Blood Lymphocytes and E.coli PQ Rec Nat Prod 2009; 3:68-75.
  • Totter JR. Spontaneous cancer and its possible relationship to oxygen metabolism. Proc Natl Acad Sci USA 1980; 77:1763-7.
  • Ames BN, Shigenaga MK, Hagen TM. Oxidants, antioxidants and the degenerative diseases of aging. Proc Natl Acad Sci USA 1993; 90:7915-22.
  • Roy N, Laskar RA, Kumari D, Ghosh T, Begum,NA. A detailed study on the antioxidant activity of the stem bark of Dalbergia sissoo Roxb., an Indian Medicinal Plant. Food Chem, 2011; 126:1115-21.
  • Koppikar SJ, Choudhari AS, Suryavanshi SA, Kumari S, Chattopadhyay S, Kaul-Ghanekar R. Aqueous Cinnamon Extract (ACE-c) from the bark of Cinnamomum cassia causes apoptosis in human cervical cancer cell line (SiHa) through loss of mitochondrial membrane potential. BMC Cancer 2010; 18:210-22.
  • Shan B, Cai YZ, Sun M, Corke H. Antioxidant capacity of 26 spice extracts and characterization of their phenolic constituents. J Agric Food Chem 2005; 5;53:7749-59.
  • Rubio L, Motilva MJ, Romero MP. Recent advances in biologically active compounds in herbs and spices: a review of the most effective antioxidant and anti-inflammatory active principles. Crit Rev Food Sci Nutr 2013; 53:943-53.
  • Craig WJ. Phytochemicals: guardians of our health. J Am Diet Assoc 1997; 97(10 Suppl 2):199-204.
  • Liu RH. Potential synergy of phytochemicals in cancer prevention: mechanism of action. J Nutr 2004; 134(12 Suppl):3479-85.
  • Kaefer CM, Milner JA. The role of herbs and spices in cancer prevention. J Nutr Biochem 2008;19:19347-61.
  • Gruenwald J, Freder J, Armbruester N. Cinnamon and Health. Crit Rev Food Sci Nutr 2010; 50:822-34.
  • Kwon HK, Jeon WK, Hwang JS, Lee CG, So JS, Park JA, Ko BS, Im SH. Cinnamon extract suppresses tumor progression by modulating angiogenesis and the effector function of CD8+ T cells. Cancer Lett 2009; 278:174-82.
  • Khan A, Safdar M, Ali Khan MM, Khattak KN, Anderson RA. Cinnamon improves glucose and lipids of people with Type 2 diabetes. Diabetes Care 2003; 26:3215–8.
  • Anderson RA. Chromium and polyphenols from cinnamon improve insulin sensitivity. Proceed Nutr Soc 2008; 67:48–53.
  • Schoene NW, Kelly MA, Polansky MM, Anderson RA. Watersoluble polymeric polyphenols from cinnamon inhibit proliferation and alter cell cycle distribution patterns of hematologic tumor cell lines. Cancer Lett 2005; 230:134-40.
  • Kamei T, Kumano H, Iwata K, Nariai Y, Matsumoto T. The effect of a traditional Chinese prescription for a case of lung carcinoma. J Alternat Complemen Med 2000; 6:557-9.
  • Singh R, Koppikar SJ, Paul P, Gilda S, Paradkar AR, Kaul-Ghanekar R. Comparative analysis of cytotoxic effect of aqueous cinnamon extract from Cinnamomum zeylanicum bark with commercial cinnamaldehyde on various cell lines. Phar Bio 2009; 47:1174-9.
  • Cao H, Urban JF Jr, Anderson RA. Cinnamon polyphenol extract affects immune responses by regulating antiand proinflammatory and glucose transporter gene expression in mouse macrophages. J Nutr 2008; 138:833-40.
  • Wondrak GT, Villeneuve NF, Lamore SD, Bause AS, Jiang Tao Z, Donna D. The Cinnamon-Derived Dietary Factor Cinnamic Aldehyde Activates the Nrf2-Dependent Antioxidant Response in Human Epithelial Colon Cells. Molecules 2010; 15:3338–55.
  • Qin B, Dawson HD, Schoene NW, Polansky MM, Anderson RA. Cinnamon polyphenols regulate multiple metabolic pathways involved in insulin signaling and intestinal lipoprotein metabolism of small intestinal enterocytes. Nutrition 2012; 28:1172-79.
  • Doyle A, Griffiths JB, Newell DG. Cell and Tissue Culture: Laboratory Procedures, John Wiley & Sons,Inc., Chichester, England 1995.
  • Singleton VL, Rossi JA. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am J Enol Vitic 1965; 16:144–53.
  • Chang C, Yang M, Wen H. Estimation of total flavonoid content in propolis by two complementary colorimetric methods, J Food Drug Anal 2002; 10:178-82.
  • Yang CH, Li RX, Chuang LY. Antioxidant activity of various parts of Cinnamomum cassia extracted with different extraction methods. Molecules 2012; 17:7294-304.
  • Kamleshiya P, Meshram VG, Ansari AH. Comparative evaluation of antioxidant and free-radical scavenging activity of aqueous and methanolic spice extracts. Int J Life Sci Pharm Res 2012; 2:118-25.
  • Jothi SL, Zuraini Z, Sasidharan S. Phytochemical screening DPPH free radical scavenging and xanthine oxidase inhibitory activities of Cassia fistula seeds extract. J Med Plant Res 2011; 5:1941-7.
  • Collins AR, Dobson VL, Dusinska M, Kenned G, Stetina R. The Comet Assay: what can it really tell us? Mutat Res 1997; 375:183–93.
  • Singh NP, Mc Coy MT, Tice RP, Schneider EL. A simple technique for quantitation of low levels of DNA damage in individual cells. Exp Cell Res 1988; 175:184-91.
  • Wong VWC, Szeto YT, Collins AR, Benzie IFF. The Comet assay: a biomonitoring tool for nutraceutical research. Curr Topics in Nutr Res 2005; 3:1–14.
  • Kwon HK, Hwang JS, So JS, Lee CG, Sahoo A, Ryu JH, Jeon WK, Ko BS, Im CR, Lee SH, Park ZY, Im SH. Cinnamon extract induces tumor cell death through inhibition of NFκB and AP1. BMC Cancer 2010; 10:392-401.
  • Jang HD, Chang KS, Huang YS, Hsu CL, Lee SH, Su MS. Principal phenolic phytochemicals and antioxidant activities of three Chinese medicinal plants. Food Chem 2007; 103:749–56.
  • Diplock AT. Antioxidant nutrients and disease prevention: an overview. Am J Clin Nutr 1991; 53:189-93.
There are 37 citations in total.

Details

Primary Language English
Journal Section Articles
Authors

Sumru Sözer Karadağlı This is me

Borte Agrap This is me

Ferzan Lermioğlu Erciyas

Publication Date February 19, 2014
Published in Issue Year 2014 Volume: 18 Issue: 1

Cite

APA Sözer Karadağlı, S., Agrap, B., & Lermioğlu Erciyas, F. (2014). Investigation of the protective effect of Cinnamomum cassia bark extract against H2O2-induced oxidative DNA damage in human peripheral blood lymphocytes and antioxidant activity. Marmara Pharmaceutical Journal, 18(1), 43-48. https://doi.org/10.12991/mpj.201414125
AMA Sözer Karadağlı S, Agrap B, Lermioğlu Erciyas F. Investigation of the protective effect of Cinnamomum cassia bark extract against H2O2-induced oxidative DNA damage in human peripheral blood lymphocytes and antioxidant activity. Marmara Pharm J. April 2014;18(1):43-48. doi:10.12991/mpj.201414125
Chicago Sözer Karadağlı, Sumru, Borte Agrap, and Ferzan Lermioğlu Erciyas. “Investigation of the Protective Effect of Cinnamomum Cassia Bark Extract Against H2O2-Induced Oxidative DNA Damage in Human Peripheral Blood Lymphocytes and Antioxidant Activity”. Marmara Pharmaceutical Journal 18, no. 1 (April 2014): 43-48. https://doi.org/10.12991/mpj.201414125.
EndNote Sözer Karadağlı S, Agrap B, Lermioğlu Erciyas F (April 1, 2014) Investigation of the protective effect of Cinnamomum cassia bark extract against H2O2-induced oxidative DNA damage in human peripheral blood lymphocytes and antioxidant activity. Marmara Pharmaceutical Journal 18 1 43–48.
IEEE S. Sözer Karadağlı, B. Agrap, and F. Lermioğlu Erciyas, “Investigation of the protective effect of Cinnamomum cassia bark extract against H2O2-induced oxidative DNA damage in human peripheral blood lymphocytes and antioxidant activity”, Marmara Pharm J, vol. 18, no. 1, pp. 43–48, 2014, doi: 10.12991/mpj.201414125.
ISNAD Sözer Karadağlı, Sumru et al. “Investigation of the Protective Effect of Cinnamomum Cassia Bark Extract Against H2O2-Induced Oxidative DNA Damage in Human Peripheral Blood Lymphocytes and Antioxidant Activity”. Marmara Pharmaceutical Journal 18/1 (April 2014), 43-48. https://doi.org/10.12991/mpj.201414125.
JAMA Sözer Karadağlı S, Agrap B, Lermioğlu Erciyas F. Investigation of the protective effect of Cinnamomum cassia bark extract against H2O2-induced oxidative DNA damage in human peripheral blood lymphocytes and antioxidant activity. Marmara Pharm J. 2014;18:43–48.
MLA Sözer Karadağlı, Sumru et al. “Investigation of the Protective Effect of Cinnamomum Cassia Bark Extract Against H2O2-Induced Oxidative DNA Damage in Human Peripheral Blood Lymphocytes and Antioxidant Activity”. Marmara Pharmaceutical Journal, vol. 18, no. 1, 2014, pp. 43-48, doi:10.12991/mpj.201414125.
Vancouver Sözer Karadağlı S, Agrap B, Lermioğlu Erciyas F. Investigation of the protective effect of Cinnamomum cassia bark extract against H2O2-induced oxidative DNA damage in human peripheral blood lymphocytes and antioxidant activity. Marmara Pharm J. 2014;18(1):43-8.