Preliminary investigations of in vitro antioxidant, antityrosinase, acetylcholinesterase and anti-inflammatory activities of Actinodaphne species

Year 2016, Volume: 20 Issue: 2, 137 - 143, 02.02.2016

Wan Mohd Nuzul Hakimi Wan Salleh , Farediah Ahmad

Abstract

Preliminary investigations of antioxidant, antityrosinase, acetylcholinesterase and anti-inflammatory activities of Actinodaphne species

Abstract

The purpose of this study was to investigate the biological activities of extracts from two Actinodaphne species from Malaysia, which are Actinodaphne macrophylla and Actinodaphne pruinosa. The bioactivity studies were determined on their antioxidant, antityrosinase, acetylcholinesterase and anti-inflammatory activities. The methanolic bark extract of A. pruinosa have shown the highest activity on DPPH (IC₅₀: 70.2 μg/mL), ABTS (IC₅₀: 147.2 μg/mL) and phenolic content (55.1 mg gallic acid/g), while the bark extracts of A. macrophylla showed the best inhibitory activity against mushroom tyrosinase (I: 45.2%) and lipoxygenase (I: 69.2%) assays. All extracts have shown strong AChE inhibitory activity (I: 70.5–88.1%), comparable to galantamine (I: 95.9%). The isolation of specific bioactive compounds as well as evaluating their safety may be necessary in the exploration of these species for potential new therapeutic drugs or drug leads.

Keywords

Actinodaphne, antioxidant, antityrosinase, acetylcholinesterase, anti-inflammatory

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