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Genotype and Allele Frequencies of 2841A>T Polymorphism in Glucose Transporter 1 gene in Turkish Population

Year 2016, Volume: 20 Issue: 2, 152 - 156, 25.02.2016

Zeliha Kayaaltı , Fezile Özdemir Dilek Kaya-akyüzlü , Tülin Söylemezoğlu

Abstract

Glucose transporter 1 (GLUT1) is located in basolateral side of cells and mediates the unidirectional transport of glucose as non-insulin dependent glucose transporter protein. It is responsible for transportation of glucose especially to cancer cells and also transports arsenic compounds that is related with diabetes mellitus. The present study aimed to detect the allele and genotype frequencies of GLUT1 2841A>T single nucleotide polymorphism (SNP) in Turkish population. Blood samples were collected from unrelated 100 male and 110 female. Polymerase Chain Reaction (PCR)-Restriction Fragment Length Polymorphism (RFLP) technique was used to analyze the blood samples. The distribution of 2841A>T SNP was 55.2%, 38.1% and 6.7% for AA, AT and TT genotype, respectively. The frequency of the A allele was found as 74.3% and those of the T allele as 25.7%. The statistical evaluation represented that the allele frequencies was accordant with Hardy- Weinberg equilibrium (χ2 =0.002, p=0.97). This was the first study examining the frequencies of GLUT1 2841A>T polymorphism in Turkish population. In our opinion, these results can be used in epidemiological studies both in Turkiye and other countries.

Keywords

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References

  • Robichaud T, Appleyard AN, Herbert RB, Henderson PJF, Carruthers A. Determinants of Ligand Binding Affinity and Cooperativity at the Glut1 Endofacial Site. Biochemistry. 2011; 50: 3137–3148.
  • Amann T, Hellerbrand C. GLUT1 as a Therapeutic target in hepatocellular carcinoma. Expert Opin Ther Targets. 2009; 13: 1411¬–1427.
  • Wood S, Trayhurn P. Glucose transporters (GLUT and SGLT): expanded families of sugar transport proteins. British Journal of Nutrition. 2003; 89: 3–9.
  • Ganapathy V, Thangaraju M, Prasad PD.Nutrient transporters in cancer: Relevance to Warburg hypothesis and beyond. Pharmacology & Therapeutics. 2009; 121: 29–40.
  • Ammann T, Kırovski G, Bosserhoff AK, Hellerbrand C. Analysis of a promoter polymorphism of the GLUT1 gene in patients with hepatocellular carcinoma. Molecular Membrane Biology. 2011; 28: 182–186.
  • Semenza GL, Wang GL. A nuclear factor induced by hypoxia via de novo protein synthesis binds to the human erythropoietin gne enhancer at a site required for transcriptional activation. Mol Cell Bio. 1992; 12: 5447–5454.
  • Semenza GL. Involvement of hypoxia- inducible factor 1 in human cancer. Intern Med. 2002; 41: 79–83.
  • Hodgkinson AD, Page T, Millward BA, Demaine AG. A novel polymorphism in the 5’ flanking region of the glucose transporter (GLUT1) gene is strongly associated with diabetic nephropathy in patients with Type 1 diabetes mellitus. Journal of Diabetes and Its Complications. 2005; 19: 65–69.
  • Cui W, Du B, Zhou W, Jia Y, Sun G, Sun J, Zhang D, Yuan H, Xu F, Lu X, Luo P, Miao L. Relationship between five Glut1 gene single nucleotide polymorphisms and diabetic nephropathy: a systematic review and meta-analysis. Mol Biol Rep. 2012; 39: 8551– 8558.
  • Mogyorosi A, Ziyadeh FN. GLUT1 and TGF- β: the link between hyperglycaemia and diabetic nephropathy. Nephrol Dial Transplant. 1999; 14: 2827–2829.
  • Islam R, Khan I, Hassan N, McEvoy M, D’Este C, Attia J, Peel R, Sultana M, Akter S, Milton A H. Association between type 2 diabetes and chronic arsenic exposure in drinking water: A cross sectional study in Bangladesh. Environmental Health. 2012; 11:38.
  • Liu Z, Sanchez M A, Jiang X, Boles E, Landfear S M, Rosen B P. Mammalian glucose Permease Glut1 facilitates transport of arsenic trioxide and methylarsonous acid. Biochemical and Biophysical Research Communications. 2006; 351:424–430.
  • International Agency for Research on Cancer. GLOBOCAN 2012: Estimated Cancer Incidence, Mortality and Prevalence Worldwide in 2012. http://globocan.iarc.fr/Pages/fact_sheets_cancer.aspx. Accessed 18 May 2015.
  • Turkey Public Health Association. Cancer Incidence. 2013. http://kanser.gov.tr/Dosya/2016_Haberler/KANSER_iNSiDANSLARi_2013_kisa_rapor.pdf. Accessed 19 Feb 2016.
  • International Diabetes Federation. IDF Diabetes Atlas Sixth edition. 2014. http://www.idf.org/sites/default/files/Atlas-poster-2014_EN.pdf. Accessed 18 May 2015.
  • Chaudhary R, Singh B, Kumar M, Gakhar SK, Saini AK, Parmar VS, Chhillar AK. Role of single nucleotide polymorphism in pharmacogenomics and their association with human diseases. Drug Metabolism Reviews. 2015. Early online 1-10. doi: 10.3109/03602532.2015.1047027
  • Kayaalti Z, Odabaşi M, Söylemezoğlu T. Genotype and allele frequencies of divalent metal transporter 1 polymorphism in Turkish population. Mol Bio Rep. 2011; 38: 2679–2684.
  • Rosen B P and Liu Z. Transport pathways for arsenic and selenium: A minireview. Environ Int. 2009; 35: 512–515.

Year 2016, Volume: 20 Issue: 2, 152 - 156, 25.02.2016

Zeliha Kayaaltı , Fezile Özdemir Dilek Kaya-akyüzlü , Tülin Söylemezoğlu

Abstract

Glukoz transporteri (Glut1) hücrenin bazolateralinde yer almaktadır ve insulinden bağımsız olarak glukozun tek yönlü taşınımını sağlar. Özellikle kanser hücrelerine glukoz taşımasından sorumlu olan Glut1 ayrıca, diyabetle ilişkili olarak arsenik bileşiklerini de taşınmaktadır. Söz konusu çalışma, türk popülasyonundaki Glut1 (a2841t) tek nükleotit polimorfizminin allel ve genotip frekansını belirlemeyi amaçlamaktadır. Birbirinden bağımsız 100 erkek

Keywords

Glukoz transporter 1 türk popülasyonu polimorfizm HpyCH4

References

  • Robichaud T, Appleyard AN, Herbert RB, Henderson PJF, Carruthers A. Determinants of Ligand Binding Affinity and Cooperativity at the Glut1 Endofacial Site. Biochemistry. 2011; 50: 3137–3148.
  • Amann T, Hellerbrand C. GLUT1 as a Therapeutic target in hepatocellular carcinoma. Expert Opin Ther Targets. 2009; 13: 1411¬–1427.
  • Wood S, Trayhurn P. Glucose transporters (GLUT and SGLT): expanded families of sugar transport proteins. British Journal of Nutrition. 2003; 89: 3–9.
  • Ganapathy V, Thangaraju M, Prasad PD.Nutrient transporters in cancer: Relevance to Warburg hypothesis and beyond. Pharmacology & Therapeutics. 2009; 121: 29–40.
  • Ammann T, Kırovski G, Bosserhoff AK, Hellerbrand C. Analysis of a promoter polymorphism of the GLUT1 gene in patients with hepatocellular carcinoma. Molecular Membrane Biology. 2011; 28: 182–186.
  • Semenza GL, Wang GL. A nuclear factor induced by hypoxia via de novo protein synthesis binds to the human erythropoietin gne enhancer at a site required for transcriptional activation. Mol Cell Bio. 1992; 12: 5447–5454.
  • Semenza GL. Involvement of hypoxia- inducible factor 1 in human cancer. Intern Med. 2002; 41: 79–83.
  • Hodgkinson AD, Page T, Millward BA, Demaine AG. A novel polymorphism in the 5’ flanking region of the glucose transporter (GLUT1) gene is strongly associated with diabetic nephropathy in patients with Type 1 diabetes mellitus. Journal of Diabetes and Its Complications. 2005; 19: 65–69.
  • Cui W, Du B, Zhou W, Jia Y, Sun G, Sun J, Zhang D, Yuan H, Xu F, Lu X, Luo P, Miao L. Relationship between five Glut1 gene single nucleotide polymorphisms and diabetic nephropathy: a systematic review and meta-analysis. Mol Biol Rep. 2012; 39: 8551– 8558.
  • Mogyorosi A, Ziyadeh FN. GLUT1 and TGF- β: the link between hyperglycaemia and diabetic nephropathy. Nephrol Dial Transplant. 1999; 14: 2827–2829.
  • Islam R, Khan I, Hassan N, McEvoy M, D’Este C, Attia J, Peel R, Sultana M, Akter S, Milton A H. Association between type 2 diabetes and chronic arsenic exposure in drinking water: A cross sectional study in Bangladesh. Environmental Health. 2012; 11:38.
  • Liu Z, Sanchez M A, Jiang X, Boles E, Landfear S M, Rosen B P. Mammalian glucose Permease Glut1 facilitates transport of arsenic trioxide and methylarsonous acid. Biochemical and Biophysical Research Communications. 2006; 351:424–430.
  • International Agency for Research on Cancer. GLOBOCAN 2012: Estimated Cancer Incidence, Mortality and Prevalence Worldwide in 2012. http://globocan.iarc.fr/Pages/fact_sheets_cancer.aspx. Accessed 18 May 2015.
  • Turkey Public Health Association. Cancer Incidence. 2013. http://kanser.gov.tr/Dosya/2016_Haberler/KANSER_iNSiDANSLARi_2013_kisa_rapor.pdf. Accessed 19 Feb 2016.
  • International Diabetes Federation. IDF Diabetes Atlas Sixth edition. 2014. http://www.idf.org/sites/default/files/Atlas-poster-2014_EN.pdf. Accessed 18 May 2015.
  • Chaudhary R, Singh B, Kumar M, Gakhar SK, Saini AK, Parmar VS, Chhillar AK. Role of single nucleotide polymorphism in pharmacogenomics and their association with human diseases. Drug Metabolism Reviews. 2015. Early online 1-10. doi: 10.3109/03602532.2015.1047027
  • Kayaalti Z, Odabaşi M, Söylemezoğlu T. Genotype and allele frequencies of divalent metal transporter 1 polymorphism in Turkish population. Mol Bio Rep. 2011; 38: 2679–2684.
  • Rosen B P and Liu Z. Transport pathways for arsenic and selenium: A minireview. Environ Int. 2009; 35: 512–515.
There are 18 citations in total.

Details

Primary Language English
Journal Section Articles
Authors

Zeliha Kayaaltı

Fezile Özdemir This is me

Dilek Kaya-akyüzlü

Tülin Söylemezoğlu

Publication Date February 25, 2016
Published in Issue Year 2016 Volume: 20 Issue: 2

Cite

APA Kayaaltı, Z., Özdemir, F., Kaya-akyüzlü, D., Söylemezoğlu, T. (2016). Genotype and Allele Frequencies of 2841A>T Polymorphism in Glucose Transporter 1 gene in Turkish Population. Marmara Pharmaceutical Journal, 20(2), 152-156. https://doi.org/10.12991/mpj.20162067368
AMA Kayaaltı Z, Özdemir F, Kaya-akyüzlü D, Söylemezoğlu T. Genotype and Allele Frequencies of 2841A>T Polymorphism in Glucose Transporter 1 gene in Turkish Population. J Res Pharm. February 2016;20(2):152-156. doi:10.12991/mpj.20162067368
Chicago Kayaaltı, Zeliha, Fezile Özdemir, Dilek Kaya-akyüzlü, and Tülin Söylemezoğlu. “Genotype and Allele Frequencies of 2841A>T Polymorphism in Glucose Transporter 1 Gene in Turkish Population”. Marmara Pharmaceutical Journal 20, no. 2 (February 2016): 152-56. https://doi.org/10.12991/mpj.20162067368.
EndNote Kayaaltı Z, Özdemir F, Kaya-akyüzlü D, Söylemezoğlu T (February 1, 2016) Genotype and Allele Frequencies of 2841A>T Polymorphism in Glucose Transporter 1 gene in Turkish Population. Marmara Pharmaceutical Journal 20 2 152–156.
IEEE Z. Kayaaltı, F. Özdemir, D. Kaya-akyüzlü, and T. Söylemezoğlu, “Genotype and Allele Frequencies of 2841A>T Polymorphism in Glucose Transporter 1 gene in Turkish Population”, J Res Pharm, vol. 20, no. 2, pp. 152–156, 2016, doi: 10.12991/mpj.20162067368.
ISNAD Kayaaltı, Zeliha et al. “Genotype and Allele Frequencies of 2841A>T Polymorphism in Glucose Transporter 1 Gene in Turkish Population”. Marmara Pharmaceutical Journal 20/2 (February 2016), 152-156. https://doi.org/10.12991/mpj.20162067368.
JAMA Kayaaltı Z, Özdemir F, Kaya-akyüzlü D, Söylemezoğlu T. Genotype and Allele Frequencies of 2841A>T Polymorphism in Glucose Transporter 1 gene in Turkish Population. J Res Pharm. 2016;20:152–156.
MLA Kayaaltı, Zeliha et al. “Genotype and Allele Frequencies of 2841A>T Polymorphism in Glucose Transporter 1 Gene in Turkish Population”. Marmara Pharmaceutical Journal, vol. 20, no. 2, 2016, pp. 152-6, doi:10.12991/mpj.20162067368.
Vancouver Kayaaltı Z, Özdemir F, Kaya-akyüzlü D, Söylemezoğlu T. Genotype and Allele Frequencies of 2841A>T Polymorphism in Glucose Transporter 1 gene in Turkish Population. J Res Pharm. 2016;20(2):152-6.

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