Cytotoxic Effect of Laurocerasus officinalis Extract on Human Cancer Cell Lines

Year 2017, Volume: 21 Issue: 1, 121 - 126, 20.09.2016

Selim Demir , Ibrahim Turan , Feyza Demir Elif Ayazoglu Demir Yuksel Aliyazicioglu

https://doi.org/10.12991/marupj.259889

Abstract

Laurocerasus officinalis is a popular black summer fruit and it belongs to Prunus genus and Rosaceae family. L. officinalis has many biological activities, such as anti-inflammatory, antinociceptive, antioxidant, neuroprotective, antidiabetic, among others. The aim of the current study was to determine the antioxidant activity and cytotoxic effects of dimethyl sulfoxide extract of L. officinalis (DEL). Total polyphenolic content and cytotoxic activity of DEL was investigated using Folin-Ciocalteu method and MTT assay, respectively. Total polyphenolic content of DEL was found 33.7±0.13 mg gallic acid equivalent/g fruit powder. DEL exhibited selective cytotoxic effect against four human cancer (lung, colon, liver and cervix) cell lines. The predominant selective cytotoxic effect of DEL was observed against colon cancer cells (IC₅₀=265.2±8.1 µg/mL) compared to normal fibroblast cells. These data demonstrate that L. officinalis extract exhibited selective cytotoxicity towards some cancer cells. To clarify the effective molecules and their mechanisms, further studies are needed to undertaken.

Keywords

Antioxidant activity, Cherry laurel, Cytotoxic effect, Laurocerasus officinalis, Polyphenolic compounds

Laurocerasus officinalis Ekstraktının İnsan Kanser Hücre Serileri Üzerindeki Sitotoksik Etkisi

Abstract

Laurocerasus officinalis Roem.
(Prunus laurocerasus L.), yerel  olarak
karayemiş veya taflan olarak da bilinir, Prunus cinsi ve  Rosaceae familyasına mensup, koyu renkli bir
yaz meyvesidir.  L. officinalis,
anti-inflamatuvar, antinosiseptif, antioksidan, 
nöroprotektif ve antidiyabetik gibi çeşitli biyolojik aktiviteler  göstermektedir. Bu çalışmanın amacı, L.
officinalis’in dimetil  sülfoksitli
ekstraktının antioksidan ve sitotoksik etkilerini  belirlemektir. Ekstraktın toplam polifenol
içeriği Folin-  Ciocalteu metodu,
sitotoksik etkinliği ise MTT yöntemiyle  belirlendi.
Ekstraktın toplam polifenol içeriği 33.7±0.13 mg  gallik asit ekivalanı/g örnek olarak bulundu.
Ekstrakt akciğer,  kolon, karaciğer ve
serviks kanser hücre serilerinde seçici  bir
sitotoksik etki gösterdi. Normal fibroblast hücreleri ile  karşılaştırıldığında en etkili seçici
sitotoksik etkinin kolon  kanseri
hücrelerinde olduğu belirlendi (IC50=265.2±8.1 μg/mL).  Sonuçlar L. officinalis ekstraktının bazı
kanser hücre serilerinde  seçici bir
sitotoksik etki gösterebildiğini ortaya koymaktadır.  Ekstrakttaki etken molekülleri ve bunların
etki mekanizmalarını  belirleyebilmek
için daha ileri çalışmalara ihtiyaç vardır.  

Keywords

Antioksidan aktivite, Karayemiş, Laurocerasus officinalis, Sitotoksik etki, Polifenolik bileşikler

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