Investigation of MBL-1 gene polymorphism in some cattle breeds raised in Turkey

Year 2019, Volume: 32 Issue: 1, 25 - 30, 01.04.2019

Esma Gamze Aksel , Korhan Arslan , Fadime Özdemir Bilal Akyüz

https://doi.org/10.29136/mediterranean.457231

Cited By: 2

Abstract

The
concern in using genetic methods in forming resistant herds against diseases in
farm animals has recently been increasing. Mannose-binding lectin-1 (MBL-1) gene is one of the genes searched
in this kind of research because of this gene's important responsibilities in
the immune system. In the presented research, Zavot (n= 81, Z), Anatolian Black
(n= 87, AB), Eastern Anatolian Red (n= 72, EAR), Turkish Gray (n= 54, TG),
Southern Anatolian Red (n= 44, SAR) which are the cattle breeds raised in
Turkey, and Black Switzerland (n= 61, BS) and Simmental (n= 65, S) which are
Europe originated cattle breeds were used as the research material. In the
research, it was aimed at genotyping the samples of cattle searched in terms of
total three single nucleotide polymorphism in the intron 1 (1252 G>A) and
exon 2 (2534 G>A, 2569 T>C) regions of MBL-1 gene. In the end of the study, it was found that Hardy
Weinberg (HWE) equilibrium broke down in EAR and S breeds (p<0.05) in terms
of 1252 G>A coded SNP; in NB and BS races (p<0.001 and p<0.01
respectively) in terms of 2534G>A coded SNP. It was determined that all
breeds are in HWE in terms of 2569 T>C coded SNP. This research is the first
research where SNPs in intron 1 (1252 G>A) and exon 2 (2534 G>A, 2569
T>C) regions of MBL-1 gene in the
cattle breeds raised in Turkey have been searched. At the end of the study, it
was thought that the studies where the relationships between SNPs and important
raising disease are searched should be planned.

Keywords

MBL-1, Cattle, PCR-RFLP, SNP

Türkiye’de yetiştirilen bazı sığır ırklarında MBL-1 gen polimorfizminin araştırılması

Abstract

Çiftlik hayvanlarında son yıllarda hastalıklara karşı daha dirençli
sürülerin oluşturulması için genetik yöntemlerin kullanılmasına yönelik ilgi
giderek artmaktadır. Bu tür çalışmalarda immun sistemde önemli görevlerinden
dolayı mannoz bağlayıcı lektin-1 (MBL-1)
geni de incelenen genlerden biridir. Sunulan çalışmada araştırma materyali
olarak Türkiye’de yetiştirilen yerli sığır ırklarından Zavot (n= 81, Z), Yerli
Kara (n= 87, YK), Doğu Anadolu Kırmızısı (n= 72, DAK), Boz ırk (n= 54, BI),
Güney Anadolu Kırmızısı (n= 44, GAK) ile Avrupa orijinli sığır ırklarından
İsviçre Esmeri (n= 61, BS) ve Simental (n= 65, S) ırkı sığırlar kullanılmıştır.
Çalışmada MBL-1 geninin intron 1
(1252 G>A) ve ekzon 2 bölgelerinde (2534 G>A, 2569 T>C) bulunan toplam
üç tek nükleotid polimorfizmi (SNP) yönünden incelenen sığır ırklarına ait
örneklerin genotiplendirilmesi hedeflenmiştir. Çalışma sonunda 1252 G>A
kodlu SNP yönünden DAK ve S ırklarında (p<0.05); 2534G>A kodlu SNP
yönünden YK ve BS ırklarında (p<0.001, p<0.01 sırasıyla) Hardy Weinberg
(HWE) dengesinin bozulduğu görülmüştür. 2569 T>C kodlu SNP yönünden ise tüm
ırkların HW dengesinde oldukları belirlenmiştir. Bu çalışma Türkiye’de
yetiştirilen sığır ırklarında MBL-1
geninin intron 1 (1252 G>A) ve ekzon 2 bölgelerinde (2534 G>A, 2569
T>C) bulunan SNP’lerin araştırıldığı ilk çalışmadır. Çalışma sonunda bu
SNP’ler ile önemli yetiştiricilik hastalıkları arasındaki ilişkilerin
araştırıldığı çalışmaların planlanması gerektiği düşünülmüştür.

Keywords

MBL-1, PCR-RFLP, SNP, Sığır

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