The Evaluation of Protein Oxidation in The Rats Which Induced Diabetes by Streptozotocin

Abstract

Aim: Diabetes mellitus (DM) is a chronic disorder and characterized by the development of long-term complications. Methylglyoxal (MGO), a precursor of advanced glycation endproducts (AGE), is detoxified in the organism by Glyoxalase through Glyoxalese I (GLO I) and GLO II.This study was aimed to investigate AGE formation in a diabetic rat model induced by streptozotocin (STZ) and the possible role of melatonin MEL which is a powerful antioxidant in this mechanism.
Materials and Methods:Four study groups, each containing ten Sprague Dawley rats, were defined as control, MEL, STZ and STZ-MEL. STZ and STZ-MEL groups were given a single 50 mg/kg dose of STZ to induce diabetes. MEL, 25 mg/kg was given intraperitoneally to MEL and STZ-MEL groups on a daily basis for 42 days. At the end of study, the levels of MGO, GLO I and GLO II enzymes were also determined in only tissue samples.
Results: Blood and urine glucose levels were found to be high in rats (p<0.05). STZ group had been shown to have higher tissue MGO levels and lower GLO I and GLO II activities (p<0.05). MEL treatment had suppressed high levels of MGO and increased enzymatic activities in STZ-MEL group.
Conclusion: In this study, we have shown that reducing MGO tissue levels in chronic diabetes to almost normal level and that the GLO system suppressed in diabetic rats are preserved with MEL,GLO I and GLO II activities increased. It has been shown that STZ induced diabetic rats had high MGO levels and the supression of GLO detoxification system indicates that AGE formation in diabetes is inevitable. Therefore, the usage of antioxidants such as MEL may be suggested to prevent diabetic complications.

Keywords

• Diabetes mellitus
• Protein Oxidation
• Methylglyoxal

Supporting Institution

This research was supported by the Scientific Research Projects Unit of Erciyes Üniversity

Project Number

TT-03-07;03/32

Streptozotosin Diyabeti Oluşturulan Ratlarda Protein Oksidasyonunun Değerlendirilmesi

Abstract

Amaç: Diabetes mellitus (DM) uzun süreli komplikasyonların gelişmesi ile karakterize kronik bir hastalıktır. İleri glikasyon son ürünleri (AGE) öncüsü olan metilglikoksal (MGO) Glioksalaz 1 (GLO 1) ve GLO 2 ile Glioksalaz tarafından organizmada detoksifiye edilmektedir. Bu çalışmada amaç streptozotosin (STZ) ile indüklenen diyabetik rat modelinde AGE oluşumunu araştırmak ve güçlü bir antioksidan olan melatonin (MEL)’in bu mekanizmadaki güçlü antioksidan rolünü araştırmaktır.
Materyal ve Metot: Her biri on adet Sprague Dawley ratları içeren dört çalışma grubu kontrol, MEL, STZ ve STZ-MEL olarak tanımlandı. STZ ve STZ-MEL gruplarına diyabeti indüklemek için tek doz 50 mg / kg STZ verildi. 25 mg / kg MEL, 42 gün boyunca MEL ve STZ-MEL gruplarına günlük intraperitoneal olarak verildi. Çalışmanın sonunda sadece doku örneklerinde MGO, GLO 1 ve GLO 2 enzimlerinin düzeyleri tespit edildi.
Bulgular: Ratlarda kan ve idrar glikoz düzeyleri yüksek bulundu (p<0.05). STZ grubunun daha yüksek doku MGO düzeylerine ve daha düşük GLO I ve GLO II aktivitelerine sahip olduğu (p<0.05) gösterilse de MEL tedavisi, yüksek MGO düzeylerini ve STZ-MEL grubunda artmış enzim aktivitelerini baskıladı.
Sonuç: Bu çalışmada, kronik diyabetteki MGO doku düzeylerinin neredeyse normale düştüğünü ve diyabetik sıçanlarda GLO sisteminin baskılanmasının MEL, GLO 1 ve GLO 2 aktiviteleri ile korunduğunu gösterdik. STZ kaynaklı diyabetik sıçanların yüksek MGO seviyelerine sahip olduğu ve GLO detoksifikasyon sisteminin baskılanmasının diyabet hastalığında AGE oluşumunun kaçınılmaz olduğu gösterilmiştir. Bu nedenle, MEL gibi antioksidanların kullanımı, diyabetik komplikasyonları önlemek için önerilebilir.

Project Number

TT-03-07;03/32

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