mersin univ saglık bilim derg Mersin Üniversitesi Sağlık Bilimleri Dergisi 1308-0830 Mersin University 10.26559/mersinsbd.1661328 Medical Pharmacology Tıbbi Farmakoloji Vasodilator effect of omentin on rat aorta and mesenteric artery: is it mediated by rho-kinase (ROCK)? Sıçan Aortası ve Mezenterik Arterinde Omentinin Vazodilatör Etkisi: Rho-Kinaz (ROCK) Aracılık Eder Mi? https://orcid.org/0009-0004-7958-2521 Mirkhanpour Behzad MERSİN ÜNİVERSİTESİ, SAĞLIK BİLİMLERİ ENSTİTÜSÜ, TIBBİ FARMAKOLOJİ (YL) (TEZLİ) https://orcid.org/0000-0001-8708-4945 Arslan Turgay TOROS ÜNİVERSİTESİ SAĞLIK HİZMETLERİ MESLEK YÜKSEKOKULU https://orcid.org/0000-0003-4117-6013 Büyükafşar Kansu MERSİN ÜNİVERSİTESİ, TIP FAKÜLTESİ, DAHİLİ TIP BİLİMLERİ BÖLÜMÜ, TIBBİ FARMAKOLOJİ ANABİLİM DALI 12 22 2025 18 3 360 369 03 19 2025 07 17 2025 Copyright © 2008, Mersin Üniversitesi Sağlık Bilimleri Dergisi 2008 Mersin Üniversitesi Sağlık Bilimleri Dergisi

Aim: One of the aims of this study was to determine whether the increased Rho kinase (ROCK) activation in various pathophysiological diseases changes after Omentin application, and to reveal whether it could be a biomarker candidate. Another aim was to determine the vasodilator effect of omentin on the aorta and superior mesenteric arteries and dose-response relationship of this effect. Method: In the study, the vasodilator effect of Omentin was shown in both aorta and mesenteric artery in isolated organ bath at doses of 1, 3, 10, 30, 100 and 300 ng/ml. Whether Omentin changed ROCK enzyme activity was shown by Elisa analysis in both aorta and mesenteric artery with 30 ng/ml Omentin dose. Results: The percentage vasodilator effect of omentin was found to be 18.434±4.54 for 1ng/ml, 32.009±7.14 for 3ng/ml, 48.268±6.35 for 10ng/ml, 54.42±8.26 for 30ng/ml, 54.673±7.61 for 100ng/ml, and 61.218±8.80 for 300ng/ml. In the mesenteric artery, it was found to be 6.044±2.55 for 1ng/ml, 23.763±5.266 for 3ng/ml, 40.050±6.122 for 10ng/ml, 51.972±7.471 for 30ng/ml, and 62.378±7.084 for 100ng/ml. EC50 values were determined as 5.81 ± 3.69 ng/ml for Omentin and 8.95 ± 11.69 ng/ml for L-NAME + Omentin. No relationship was found between Omentin and ROCK in terms of vascular vasoactivity. Conclusion: Although omentin is a promising adipokine for preventing and/or correcting hypertension, which occurs as a complication of diseases such as obesity and diabetes, further clinical and preclinical studies are needed.

Amaç: Bu çalışmanın amaçlarından bir tanesi, çeşitli patofizyolojik hastalıklarda artan Rho kinaz (ROCK) aktivasyonunun Omentin uygulaması sonrası değişip değişmediğini gösterip bir biyobelirteç adayı olup olmayacağını ortaya koymak. Bir diğer amacımız ise omentinin aorta ve süperior mezenterik arterlerde vazodilatör etkisi ve bu etkinin maksimum konsantrasyon doğrusunun belirlenmesidir. Yöntem: Çalışmada, Omentinin vazodilatör etkisi 1, 3, 10, 30, 100 ve 300 ng/ml dozlarında izole organ banyosunda hem aorta hem de mezenterik arterde gösterilmiştir. Omentinin ROCK enzimi aktivitesini değiştirip değiştirmediği ise 30 ng/ml Omentin dozu ile hem aortada hem de mezenterik arterde Elisa analizi ile gösterilmiştir. Bulgular: Omentin yüzde vazodilatör etkisi, 1ng/ml: için 18.434±4.54, 3ng/ml için 32.009±7.14, 10ng/ml için 48.268±6.35, 30ng/ml için 54.42±8.26, 100ng/ml için 54.673±7.61 ve 300ng/ml için 61.218±8.80 bulunmuştur. Mezenterik arterde, 1ng/ml: için 6.044±2.55, 3ng/ml için 23.763±5.266, 10ng/ml için 40,050±6,122, 30ng/ml için 51.972±7.471 ve 100ng/ml için 62.378±7.084 bulunmuştur. EC50 değerleri ise Omentin için 5.81 ± 3.69 ng/ml L-NAME + Omentin için ise 8.95 ± 11.69 ng/ml olarak tespit edilmiştir. Omentin ile ROCK arasında damar vazoaktivitesi yönünden bir ilişki bulunmamıştır. Sonuç: Omentinin, obezite, diyabet gibi hastalıkların bir komplikasyonu olarak ortaya çıkan hipertansiyonu önlemeye ve/veya düzeltmeye yönelik umut vaat eden bir adipokin olmakla birlikte daha fazla klinik ve preklinik çalışmalara ihtiyaç duyulmaktadır.

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