mersin univ saglık bilim derg

Mersin Üniversitesi Sağlık Bilimleri Dergisi

1308-0830

Mersin University

10.26559/mersinsbd.423103

Health Care Administration

Sağlık Kurumları Yönetimi

Vitamin D reseptör geni fokI polimorfizminin temporomandibular eklem dejenerasyonu ile ilişkisi

The relation between Vitamin D receptor gene fokI polymorphism and degeneration of the temporomandibular joint

https://orcid.org/0000-0002-8111-2594

Yılmaz

Ayça Dilara

ANKARA ÜNİVERSİTESİ, DİŞ HEKİMLİĞİ FAKÜLTESİ

04 28 2019

12 1 72 79 05 12 2018 11 23 2018

2008

Mersin Üniversitesi Sağlık Bilimleri Dergisi

Amaç: Temporomandibular EklemDüzensizliği (TME-İD) ve eklem osteoartritine (TME-OA) yatkınlık genetikvaryasyonlarla ilişkili olabilir. Vitamin D Reseptörü (VDR) gen polimorfizmleri buna adaydırlar.Bu çalışmanınamacı, VDR FokI polimorfizminintemporomandibulareklem dejenerasyonu ile ilişkili olup olmadığınıcinsiyete göre değerlendirmekti. Yöntem: 58 osteoartritikTME-İD hastası (32.07.±8.1) ve kontrol olarak 71 TME-İD olmayan birey(28.28±5.95)çalışmaya dahil edildi. Kan örneklerindenDNA izolasyonu standart proteinazK/fenol-kloroformmetodu ile yapıldı. VDR geni FokI polimorfizmi, polimeraz zincir reaksiyonunu (PZR) takiben restriksiyonfragment uzunlukpolimorfizmi(RFLP) yöntemi ilearaştırıldı. Bulgular:FokI genotipdağılımları(rs2228570, C>T) grupları arasında istatistiksel olarakfarklı idi (p=0.026, χ²=7.2). Heterozigot Ff genotipi, FF genotipine göre istatistiksel olarak farklı idi (OR=0.43, %95 GA:0.2-0.92, p=0.028). TME-İD’li kadınlarda heterozigot Ffgenotipi FF genotipine göre farklılık sınırında idi (OR=0.43, %95 CI:0.16-1.10, p=0.07). Yine TME-İD’li kadınlarda ff genotipiile FF genotipi arasında fark olmamasına rağmen (%95 GA: 0.29-26.03, p=0.37) risk faktörü 2.77 kat fazla idi. TME-İD/kontrol grubu ve kadın kontrollerde de F ve f alellerinin dağılımları farklı değildi. Sonuç: FokI polimorfizmininTME-İD/TMEOA gelişmesi üzerinde önemli bir etkisi olduğu düşünülebilir. ffgenotipi, TME-İD hastalarında ve TME-İD’li kadınlarda temporomandibular eklemdejenerasyonu ile ilgili olabilir.

Objective: Predisposition to temporomandibular jointinternalderangement (TMJ-ID) and osteoarthritisof the joint(TMJOA)might be related to genetic variations.Vitamin D receptor (VDR) genepolymorphismsare the candidates forassociation with the disorder. The aim ofthis study was to evaluatewhether VDRFokI polymorphism is associatedwith the degeneration of the temporomandibular jointbygender.Methods: The study included 58 unrelated TMJ-ID patients(32.07±8.1) and 71 healthy controls (28.28 ±5.95) without TMJ-ID. DNA extractionwas achieved by standardproteinaseK/phenol-chloroformmethodfrom the blood samples.VDR gene FokI polymorphism was investigatedby a polymerasechain reaction(PCR) based restrictionfragment length polymorphism(RFLP) assay.Results: The genotype distributionsof FokI (rs2228570, C>T) showed statisticallysignificant results (p=0.026,χ²=7.2). Ff heterozygote genotype was statistically different as compared to FF genotype (OR=0.43, 95% CI: 0.2-0.92,p=0.028).HeterozygoteFf genotypewas differentcomparing to the FFgenotype in TMJ-ID women (OR=0.43, 95% CI: 0.16-1.10,p=0.07).Although the difference between ff and FF genotypewere not different, ff genotype was a 2.77 foldrisk factor(95% CI:0.29-26.03, p=0.37).The distributions of F and f alleles were not different between the groups ofoverall TMJ-ID vs. controls and women TMJ-ID vs. controls. Conclusion: The results of this study suggested that FokI polymorphism mightpresent susceptibility to TMJ-ID/TMJOA. ff genotype mightbe associatedwith the degeneration of temporomandibular joint in TMJ-ID patients and TMJ-ID women.

temporomandibular eklem osteoartriti Temporomandibular eklem internal düzensizliği D Vitamini reseptör geni FokI polimorfizm

Temporomandibular joint internal derangement temporomandibular joint osteoarthritis vitamin D receptor gene FokI polymorphism

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