mersin univ saglık bilim derg Mersin Üniversitesi Sağlık Bilimleri Dergisi 1308-0830 Mersin University 10.26559/mersinsbd.489911 Health Care Administration Sağlık Kurumları Yönetimi The effects of stress and menopause on learning Stres ve menopozun öğrenme üzerindeki etkileri https://orcid.org/0000-0003-4219-8054 Berköz Mehmet https://orcid.org/0000-0002-5287-8806 Hatungil Fatma Rezan https://orcid.org/0000-0002-3351-6885 Yalın Ali Erdinç https://orcid.org/0000-0001-8060-6333 Çömelekoğlu Ülkü https://orcid.org/0000-0002-1286-2172 Yalın Serap MERSİN ÜNİVERSİTESİ https://orcid.org/0000-0002-6219-4694 Beydağı Hüseyin https://orcid.org/0000-0003-2703-6831 Reşitoğlu Bora https://orcid.org/0000-0003-2357-8262 Şahin Nefise Özlen https://orcid.org/0000-0002-6462-6841 Eroğlu Pelin 08 30 2019 12 2 210 219 11 29 2018 12 28 2018 Copyright © 2008, Mersin Üniversitesi Sağlık Bilimleri Dergisi 2008 Mersin Üniversitesi Sağlık Bilimleri Dergisi

Aim: Inthis study, we aimed to to investigate the co-effect of stress and menopause onthe learning ability in rats. Methods: 60 female Wistar-Albinorats were used in this study and wererandomly divided into four groups (15 rats per group) as follows: Control group(Group I), experimental immobilization stress model (Group II), experimentalmenopause model (Group III), and experimental menopause model + experimentalstress model (Group IV). Long-term cognitive and motor abilities of all ratswere evaluated by conducting behaviour experiments (open field and morris watermaze test). Furthermore, the gene expression of corticosteroid receptor(glucocorticoid receptor and mineralocorticoid receptor) wasexamined by Real-Time PCR using SYBR Green followed by mRNA isolation and cDNAsynthesis. Results: In the comparison study ofin-group time for attaining mean elevation over days, it was detected that timefor attaining mean elevation was statistically significantly shorter thanprevious day from 1st day to 4th(p<0.001). There was no statisticallysignificance for daily time for mean elevation among the groups (p>0.05). Inthe open area experiment, although the Group II exhibited shorter mean whencomparing the progressed distances, but the difference among the groups was notstatistically significant (p>0.05). Gene expression of both glucocorticoidreceptor and mineralocorticoid receptor in the hipocampal tissue was determinedto be lower in comparison with the control group. However, this decrease wasnot statistically significant (p>0.05). Conclusion: Thisresult shows that acute immobilization stres and experimental menopause modelis not efficient on the learning ability in rats.

Amaç: Buçalışmada stres ve menopozun birlikte, öğrenme işlevi üzerindeki etkileriaraştırılmıştır. Yöntem: Çalışmada,60 adet dişi Wistar-Albino sıçan kullanılmıştır. Bu sıçanlar randomize olarak kontrol(Grup I), deneysel immobilizasyon stres modeli (Grup II), deneysel menopozmodeli (Grup III) ve deneysel menopoz modeli + deneysel stres modeli (Grup IV)olmak üzere her birgrupta 15’er hayvan bulunan 4 gruba ayrılmıştır. Tüm sıçanların uzun dönemdekibilişsel ve motor yetileri davranış deneyleri (açık alan ve Morris su tankı)ile değerlendirilmiştir. Ayrıca hipotalamus-hipofiz-adrenal ekseniningeribildirim işlevinde merkezi konumunda olan kortikosteroid reseptör(Glukokortikoid reseptörü ve mineralokortikoid reseptörü) gen ekspresyonu mRNAizolasyonu ve cDNA sentezi takibinde SYBR Green kullanılarak eş zamanlı PCRyöntemiyle incelenmiştir. Bulgular: Tüm grupların kendi içinde günlere göre ortalamayükseltiyi bulma sürelerinin karşılaştırmasında, birinci günden 4. güne kadar,tüm gruplarda her günün ortalama yükseltiyi bulma sürelerinin bir önceki gündenistatistiksel olarak anlamlı düzeyde kısa olduğu saptanmıştır (p<0.001).Güne göre yükseltiyi bulma sürelerinin gruplar arasında karşılaştırmasındaistatistiksel olarak anlamlı bir fark saptanmamıştır (p>0.05). Açık alandeneyinde ortalama katedilen mesafenin gruplara göre karşılaştırmasında grup IIen düşük ortalamaya sahip olmasına karşın, gruplar arasında istatistiksel olarakanlamlı bir fark bulunamamıştır (p>0.05). Hipokampal dokuda hem glukokortikoidreseptörünün hem de mineralokortikoidreseptörünün gen ekspresyonu tüm gruplardakontrol grubuna kıyasla düşük bulunmuştur. Ancak bu düşüş istatistiksel olarakanlamlı bulunamamıştır (p>0.05). Sonuç:Bu sonuç akut immobilizasyon stresin ve deneysel menopoz modelinin öğrenmeüzerinde etkili olmadığını düşündürmüştür.

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