mersin univ saglık bilim derg Mersin Üniversitesi Sağlık Bilimleri Dergisi 1308-0830 Mersin University 10.26559/mersinsbd.898835 Health Care Administration Sağlık Kurumları Yönetimi Therapeutic effects of rosmarinic acid in alloplastic bone graft applied rat calvarial bone defect model Alloplastik kemik grefti uygulanmış sıçan kalvarial kemik defekt modelinde rosmarinik asidin terapötik etkileri https://orcid.org/0000-0002-7713-6681 Deveci Busra DİCLE ÜNİVERSİTESİ, DİŞ HEKİMLİĞİ FAKÜLTESİ https://orcid.org/0000-0002-1000-1379 Dağ Ahmet DİCLE ÜNİVERSİTESİ, DİŞ HEKİMLİĞİ FAKÜLTESİ https://orcid.org/0000-0002-4129-0564 Kadiroğlu Ela Tules DİCLE ÜNİVERSİTESİ, DİŞ HEKİMLİĞİ FAKÜLTESİ https://orcid.org/0000-0002-6384-9146 Aşır Fırat Dicle Üniversitesi Tıp Fakültesi https://orcid.org/0000-0002-1967-4844 Gokalp-ozkorkmaz Ebru Dicle Üniversitesi Tıp Fakültesi https://orcid.org/0000-0002-2353-1184 Deveci Engin Dicle Üniversitesi Tıp Fakültesi 04 30 2022 15 1 88 99 03 18 2021 11 09 2021 Copyright © 2008, Mersin Üniversitesi Sağlık Bilimleri Dergisi 2008 Mersin Üniversitesi Sağlık Bilimleri Dergisi

Aim: Many reasons such as trauma, neoplasms, infections, and congenital anomalies can cause calvarial bone defects. Various graft types have been tried and also treatment methods have been investigated in order to eliminate bone defects in the cranial region and to stimulate bone regeneration. In our study, we aimed to investigate the effects of Rosmarinic acid (RA) on the rat calvarial bone defect model with graft material. Method: Wistar male rats (n=32) were divided into 4 in the study; defect group as control (n=8), defect + RA treated (n=8), defect + graft (n=8), defect+graft+RA group (n=8). Under anesthesia, the frontal bone was opened and the periosteal flap was removed using a periosteal lever, and a circular full-thickness bone defect (5 mm) was created in the midline. In groups 2 and 3, allograft material was placed in the defect area, the tissue was sutured and closed. RA (100mg/kg) was administered orally for 7 days. After 28 days, all animals were sacrificed and calvarial bones were removed, fixed with 10% formalin, decalcified with 5% Ethylenediamine tetraacetic acid (EDTA), cut sections 4-6 μm thick after routine histological preparation and stained with Hematoxylin and eosin (HE). Bone Morphogenetic Protein-2 (BMP-2) was immunohistochemically labeled and examined under a light microscope. Alkaline phosphatase (ALP), creatine kinase (KK), total antioxidant level (TAS), total oxidant level (TOS) and oxidative stress index (OSI) parameters were studied from blood samples. Results: TAS value was found to be significantly higher in the defect + graft + RA group compared to the defect group (p<0.05). The OSI value was significantly lower in the defect + graft+RA group compared to the defect group. It was found that inflammatory cells were scattered in a small number of solitary forms within the graft area, and dilatation and congestion in blood vessels were decreased. It was observed that the bone tarbecules near the main bone area were enlarged, the matrix structure in which osteoblastic activity became evident and the osteocytes has settled in the lacunae. Conclusion: As a result, it was observed that with RA+Graft application, induced osteoblastic activity in the calvarial defect model, resulted in osteocyte formation and new bone development.

Amaç: Travma, neoplazmalar, enfeksiyonlar ve doğuştan anomaliler gibi pek çok neden, kalvaryal kemik kusurlarına neden olabilir. Kraniyal bölgedeki kemik kusurlarını gidermek ve kemik yenilenmesini uyarmak için çeşitli greft tipleri denenmiş ve tedavi yöntemleri araştırılmıştır. Çalışmamızda Rosmarinik asidin (RA) greft materyali uygulanmış sıçan kalvaryal kemik defekt modeli üzerindeki etkilerinin araştırılması amaçlanmıştır. Yöntem: Çalışmada Wistar erkek sıçanlar (n=32) dörde bölündü; kontrol olarak defekt grubu (n=8), defekt+RA uygulamalı grup (n=8), defekt+greft (n=8), defekt+greft+RA uygulamalı grup (n=8). Anestezi altında frontal kemik açılarak ve periosteal elevatör ile periosteal flep çıkarıldı ve orta hatta dairesel tam kalınlıkta kemik defekti (5 mm) oluşturuldu. Grup iki ve üçte defekt bölgesine allogreft materyal yerleştirildi, doku dikilerek kapatıldı. RA (100mg/kg) oral yolla 7 gün süre ile uygulandı. 28 gün sonra tüm hayvanlar sakrifiye edildi ve kalvaryal kemikler çıkarıldı, %10’luk formalin ile tespit edildikten sonra, %5’lik EDTA (Etilendiamin tetraasetik asit) ile dekalsifiye edildi, rutin histolojik preparasyondan sonra 4-6 μm kalınlığında kesitler kesildi ve Hematoksilen Eosin ile boyandı. BMP-2 (Bone morphogenetic proteins) kemik morfogenetik proteinleri immunohistokimyasal olarak işaretlendi ve ışık mikroskobu altında incelendi. Kan örneklerinden alkalen fosfataz (ALP), kreatin kinaz (KK), total antioksidan seviyesi (TAS), total oksidan seviyesi (TOS) ve oksidatif stres indeksi (OSI) parametreleri çalışıldı. Bulgular: TAS değeri defekt+greft+RA grubunda defekt grubuna göre anlamlı olarak yüksek bulunmuştur (p<0.05). OSI değeri defekt+greft+RA grubunda defekt grubuna göre anlamlı olarak düşüktür. Greft alanı içinde inflamatuvar hücrelerin az sayıda soliter olarak dağıldığı, kan damarlarında dilatasyon ve konjesyonun azaldığı tespit edildi. Ana kemik bölgesine yakın kemik tarbeküllerin genişlediği osteoblastik aktivitenin belirginleştiği matriks yapısının olgunlaştığı ve osteositlerin laküna içinde yerleştiği görüldü. Sonuç: Sonuç olarak, RA+Greft uygulaması ile kalvariyal defekt modelinde osteoblastik aktiviteyi indükleyerek osteosit oluşumuna ve yeni kemik gelişimine neden olduğu gözlenmiştir.

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