Bacterial diversity associated with the Hatay yellow strain silkworm (Bombyx mori L.): Isolation, identification and characterization

Year 2023, Volume: 28 Issue: 3, 593 - 605, 18.12.2023

Dönüş Gençer , Hande Bayraktar Şalvarcı , Başak Ulaşlı , Feza Can Cengiz , İsmail Demir

https://doi.org/10.37908/mkutbd.1288460

Cited By: 1

Abstract

The Hatay yellow strain silkworm (Bombyx mori L.), which is in danger of extinction, is one of the most important local cultural heritages of Türkiye. Bacterial pathogens of silkworm are highly destructive and cause mostly acute diseases. The aim of this study was to determine the bacterial diversity and potential pathogenic bacterial species in infected and dead larvae of Hatay yellow race. A total of 16 bacterial isolates from Hatay yellow race were identified according to their morphological, biochemical and molecular characteristics. The bacterial isolates isolated from infected and dead larvae of Hatay yellow race were Staphylococcus sp. (BM-1), Staphylococcus xylosus (BM-5), Staphylococcus succinus (BM-7), Bacillus thuringiensis (BM-8), Bacillus subtilis (BM-9), Bacillus sp. (BM-10), Staphylococcus saprophyticus (BM-16, BM-19), Klebsiella sp. (BM-17), Staphylococcus arlettae (BM-18), Pseudomonas aeruginosa (BM-20), Enterococcus mundtii (BM-21), Pantoea agglomerans (BM-22), Kluyvera intermedia (BM-23), Serratia sp. (BM-24), Mammaliicoccus sciuri (BM-25). The high bacterial density and number of species indicate that Hatay yellow race is highly susceptible to bacterial diseases. Insecticidal activity studies revealed that species belonging to Bacillus and Staphylococcus genera are important pathogens of hybrid silkworm culture and Hatay yellow race.

Keywords

Silkworm Hatay yellow strain, Bombyx mori L., Domestic silkworm breed, Pathogen bacteria

Ethical Statement

Ethical approval is not applicable, because this article does not contain any studies with human or animal subjects.

Supporting Institution

Hatay Mustafa Kemal University Research Foundation

Project Number

21.GAP.001

Thanks

We would like to thank Mrs. Emel DUMAN and Mr. Fikret DUMAN, who provided the opportunity to breed silkworms in their personal production facilities in Harbiye district of Hatay province for their great support and also we would like to thank Mr. Onur BAYRAKTAR for helping to supply mulberry leaves for raising silkworms in Trabzon. This study was supported by the Hatay Mustafa Kemal University Research Foundation (grant number 21.GAP.001).

Hatay sarısı ırkı ipekböceği (Bombyx mori L.) ile ilişkili bakteri çeşitliliği: İzolasyon, teşhis ve karakterizasyon

Abstract

Nesli tükenme tehlikesiyle karşı karşıya olan Hatay sarı ırkı ipekböceği (Bombyx mori L.), Türkiye'nin en önemli yerel kültür miraslarından biridir. İpekböceğinin bakteriyel patojenleri son derece yıkıcı olup, çoğunlukla akut hastalıklara neden olurlar. Bu çalışmanın amacı, Hatay sarı ırkının enfekteli ve ölü larvalarında bakteri çeşitliliğini ve potansiyel patojenik bakteri türlerini belirlemektir. Hatay sarı ırkından izole edilen toplam 16 bakteri izolatı morfolojik, biyokimyasal ve moleküler özelliklerine göre tanılanmıştır. Hatay sarı ırkının enfekteli ve ölü larvalarından izole edilen bakteri izolatları Staphylococcus sp. (BM-1), Staphylococcus xylosus (BM-5), Staphylococcus succinus (BM-7), Bacillus thuringiensis (BM-8), Bacillus subtilis (BM-9), Bacillus sp. (BM-10), Staphylococcus saprophyticus (BM-16, BM-19), Klebsiella sp. (BM-17), Staphylococcus arlettae (BM-18), Pseudomonas aeruginosa (BM-20), Enterococcus mundtii (BM-21), Pantoea agglomerans (BM-22), Kluyvera intermedia (BM-23), Serratia sp. (BM-24), Mammaliicoccus sciuri (BM-25) olarak belirlenmiştir. Bakteri yoğunluğu ve tür sayısının fazla olması Hatay sarı ırkının bakteriyel hastalıklara karşı son derece duyarlı olduğunu göstermektedir. İnsektisidal etkinlik çalışmaları, Bacillus ve Staphylococcus cinslerine ait türlerin hibrit ipekböceği kültürü ve Hatay sarı ırkında önemli patojenleri olduğunu ortaya koymuştur.

Keywords

İpekböceği Hatay sarı suşu, Bombyx mori L., Yerli ipekböceği cinsi, Patojen bakteri

Project Number

21.GAP.001

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