Çerezlik karpuz genotiplerinin genetik çeşitliliğinin SRAP markırları kullanılarak belirlenmesi

Yıl 2024, Cilt: 29 Sayı: 1, 29 - 37, 08.04.2024

Ömer Faruk Coşkun , Seher Toprak , Kazım Mavi

https://doi.org/10.37908/mkutbd.1359989

Öz

Karpuz (Citrullus lanatus L.) dünyada en çok yetiştirilen ve tüketilen sebzelerden biridir. Önemli bir varyasyona sahip olan karpuzun bazı genotipleri, tohum özelliklerinden dolayı çerezlik potansiyele sahiptir. Bu çalışmada, bazı çerezlik karpuz genotipleri arasındaki genetik ilişkinin belirlenmesi amacıyla SRAP (Sequence Related Amplified Polymorphism) markır tekniği kullanılmıştır. Toplam 24 genotipte, toplam 166 bant elde edilmiş ve polimorfizm oranı %97.4 olarak hesaplanmıştır. Kümeleme analizinde dört ana küme ortaya çıkmıştır. 2 ve 7 numaralı genotiplerin diğerlerinden ayrı kümelendiği belirlenmiştir. Structure analizi sonuçları genotiplerin iki alt popülasyondan oluştuğunu ortaya çıkarmıştır. Çerezlik karpuz genotiplerinin SRAP teknikleri ile genetik olarak ayırt edilebileceği sonucuna varılmıştır. Bu çalışma sonuçları çerezlik karpuz çeşit ıslahına yönelik ıslah stratejilerinde kullanılabilir.

Anahtar Kelimeler

Citrullus lanatus, Çerezlik Karpuz, Moleküler Karakterizasyon, SRAP

Destekleyen Kurum

Hatay Mustafa Kemal University

Proje Numarası

21.GAP.019

Teşekkür

This study was supported by Hatay Mustafa Kemal University Scientific Research Projects (BAP) with the project numbered 21.GAP.019.

Determination of genetic diversity of edible-seeded watermelon genotypes using SRAP markers

Öz

The watermelon (Citrullus lanatus L.) is one of the most commonly grown and consumed vegetables in the world. Some genotypes of watermelon, which have significant variations, have a snack potential due to their seed characteristics. In this study, SRAP (Sequence Related Amplified Polymorphism) marker technique was used to determine the genetic relationship between some edible-seeded watermelon genotypes. A total of 166 bands were obtained in 24 genotypes and the polymorphism rate was calculated as 97.4%. Four main clusters were observed in the cluster analysis. It was determined that genotypes 2 and 7 clustered separately from the others. Structure analysis revealed that the genotypes consisted of two subpopulations. It was concluded that the edible-seeded watermelon genotypes can be genetically differentiated by the SRAP techniques. The results of this study can be used in breeding strategies for the improvement of the edible-seeded watermelon cultivars.

Anahtar Kelimeler

Citrullus lanatus, Edible-Seeded Watermelon, Molecular Characterization, SRAP

Proje Numarası

21.GAP.019

Teşekkür

This study was supported by Hatay Mustafa Kemal University Scientific Research Projects (BAP) with the project numbered 21.GAP.019. The abstract of the study was presented at the conference of 6th UTAK 2023.

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