KLEMENTİN ESANSİYEL YAĞININ ANTİMİKROBİYAL AKTİVİTESİ İLE NIH-3T3 FİBROBLAST HÜCRELERİ ÜZERİNE SİTOTOKSİK VE İN VİTRO YARA İYİLEŞME POTANSİYELİNİN BELİRLENMESİ

Year 2018, Volume: 4 Issue: 2, 143 - 147, 19.12.2018

Tuba Baygar , Nurdan Saraç

https://doi.org/10.22531/muglajsci.435610

Cited By: 2

Abstract

Narenciye esansiyel yağları cilt ve saç bakımı,
uykusuzluk, sindirimin düzenlenmesi ve bağışıklık sisteminin geliştirilmesi
gibi pek çok alanda kullanıma sahiptir. Bu çalışma, Türkiye’de yetiştiriciliği
yapılmakta olan bir mandalina türü olan klementin’in (Citrus clementina Hort. ex Tan) kabuğundan elde edilen esansiyel
yağın biyolojik aktivitelerinin
belirlenmesi amacıyla planlanmıştır. Klementin kabuk esansiyel yağının (CPEO)
antimikrobiyal aktivitesi E. coli, S. aureus ve bir maya türü olan C.
albicans’a karşı disk difüzyon yöntemi ile belirlenmiştir. Sitotoksisitesi MTT
kolorimetrik analizi ile NIH-3T3 fibroblast hücreleri kullanılarak tespit
edilmiştir. Ayrıca stratch-yara iyileşme tekniği kullanılarak CPEO’nın
fibroblast hücrelerinin göçü ve proliferasyonu üzerine etkinliği in vitro yara
iyileşme aktivitesi olarak değerlendirilmiştir. Sonuçlara göre CPEO’nın kayda
değer antimikrobiyal aktiviteye sahip olduğu gözlenmiştir. En yüksek inhibisyon
zonu C. albicans’a karşı 20.67±0.58
mm olarak ölçülmüştür. Sitotoksik aktivite analizine göre IC50
değeri 52.50 µg/mL olarak
belirlenmiştir. CPEO için IC50 değerinin altında uygulanan
dozlarda fibroblast hücreleri kontrol grubuna kıyasla daha hızlı sürede
proliferasyon sağlayarak yapay yaranın iyileşmesine yönelik yüksek hareket
göstermiştir. Mevcut çalışma sonuçlarına göre CPEO, patojenik
mikroorganizmalara karşı sahip olduğu antimikrobiyal karakterin yanı sıra cilt
uygulamaları ve yara iyileşmeye yönelik ürünlerin geliştirilmesinde kullanılma
potansiyeline sahiptir.

Keywords

Klementin, atimikrobiyal, sitotoksisite, yara iyileşme, fibroblast

ANTIMICROBIAL ACTIVITY OF CLEMENTINE PEEL ESSENTIAL OIL WITH ITS CYTOTOXIC AND IN VITRO WOUND HEALING POTENTIAL ON NIH-3T3 FIBROBLAST CELLS

Abstract

Citrus essential
oils has many area of use such as skin and hair care, insomnia, improving
digestion, and boosting immune system. The present study is aimed to determine
the biological activities of clementine (Citrus clementina Hort. ex Tan), one of the mandarin species,
peel essential oil (CPEO) that grow in Turkey. Antimicrobial activity of CPEO
was evaluated using disc diffusion method against Escherichia coli,
Staphylococcus aureus, and a fungi Candida albicans. The MTT (3-[4,
5-dimethylthiazol-2-yl]-2, 5 diphenyltetrazolium bromide)-colorimetric monocyte
mediated cytotoxicity assay was applied using NIH-3T3 cells and IC50
value was calculated. CPEO was also evaluated for its in vitro wound healing
effect on the fibroblast cell migration and proliferation using scratch wound
assay technique. Results of the present study indicated that CPEO has
remarkable antimicrobial activity. The highest inhibition zone was observed
against C. albicans as 20.67±0.58 mm. IC50 value obtained for CPEO was 52.50 µg/mL. Fibroblast cells showed higher
migration than the control group to heal the artificial scar after treatment of
CPEO concentrations below the IC50 dose. The results of the present study indicated that the CPEO may be useful in
effective management of skin care applications and wound healing products with
its potent activity against pathogen microorganisms.

Keywords

Clementine, antimicrobial, cytotoxicity, wound healing, fibroblast

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