Sıçan PKU Modeli Cinsiyete Dayalı Nöroinflamatuar Değişiklikler Gösterir: Proinflamatuar Sitokinler ve Lipid Peroksidasyonu

Year 2024, Volume: 11 Issue: 1, 30 - 37, 30.04.2024

Çiğdem Çiçek Müslüm Gök Ebru Bodur

https://doi.org/10.47572/muskutd.1388547

Abstract

Fenilketonüri (PKU), amino asit metabolizmasının konjenital kusurlarından kaynaklanır. Birikmiş fenilalanin kan-beyin bariyerini geçer ve kalıcı beyin hasarına neden olur, ancak fenilketonürinin altında yatan nöro-patofizyoloji tam olarak anlaşılamamıştır. Prefrontal kortekste inflamatuar yanıtın, lipit peroksidasyonunun ve oksidatif stresin rolünü incelemek için her iki cinsiyete ait kimyasal olarak indüklenmiş sıçan Fenilketonüri modeli oluşturuldu. Sonuçlarımız Fenilketonüride kontrollere kıyasla lipit peroksidasyonunda artış olduğunu gösterdi; bu artış sadece erkeklerde anlamlı derecede farklıydı (p<0.001). Erkek sıçan PKU gruplarında serum triptofan (p<0.001) ve interlökin-1β düzeylerinde (p=0.014) erkek kontrollere göre anlamlı farklılıklar gözlendi. Bu çalışma ile cinsiyete ilk kez bir PKU modelinde cinsiyete bağlı nöroinflamasyon ve lipid peroksidasyonunda değişiklikler rapor edilmiştir.

Keywords

Cinsiyete Temelli, Fenilketonüri, Nöroinflamasyon, Oksidatif Stres, Proinflamatuar Sitokinler

Project Number

2018/02-12

Rat PKU Model Display Gender-Based Neuroinflammatory Changes: Proinflamatuary Cytokines and Lipid Peroxidation

Abstract

Phenylketonuria (PKU) results from congenital defects of amino acid metabolism. Accumulated phenylalanine crosses the blood-brain barrier and causes permanent brain damage, but the neuro-pathophysiology underlying phenylketonuria is not fully understood. Chemically-induced rat phenylketonuria model of both genders was generated to examine the role of inflammatory response, lipid peroxidation and oxidative stress in the prefrontal cortex. Our results showed that in phenylketonuria there was an increase in lipid peroxidation compared to controls, which was significantly different only in males (p<0.001). In male rat PKU groups, statistically significant differences were also observed in serum tryptophan (p<0.001) and interleukin-1β levels (p=0.014) as compared to male controls. In this study, gender-based changes in neuroinflammation and lipid peroxidation were reported for the first time in a PKU model.

Keywords

Gender Based, Phenylketonuria, Neuro-inflammation, Oxidative Stress, Proinflammatory Cytokines

Ethical Statement

The procedures were approved by Hacettepe University Animal Experiments Local Ethics Committee (Date: 23/08/2022, Protocol No 2022/07-16)

Supporting Institution

Hacettepe University

Project Number

2018/02-12

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