Effect of Blood Sample Tubes on Diagnosis of Gestational Diabetes in Pregnant Women Undergoing OGTT

Year 2024, Volume: 11 Issue: 3, 165 - 172, 18.12.2024

Duygu Teksöz , Nilgün Işıksaçan , Tamay Seda Taşçı , Pınar Kasapoğlu , Ramazan Murat Koşer

https://doi.org/10.47572/muskutd.1479380

Abstract

Performing a diagnostic oral glucose tolerance test (OGTT) during pregnancy is important for early diagnosis of gestational diabetes mellitus. Hyperglycemia can be detected quickly, and mother and the fetus can be protected. This study aimed to research the relationship between blood sample tubes and the stability of glucose in pregnant women undergoing OGTT. Blood samples were taken at the 120th minute after glucose loading from 20 pregnant volunteers who underwent OGTT (75g). VACUETTE CAT Serum Separator (Clot Activator Tube) was used for serum; VACUETTE FC Mix tube (Na2 EDTA, sodium fluoride, sodium citrate) and VACUETTE FE (Sodium Fluoride/K3 EDTA) were used for plasma. All three of these tubes were centrifuged at three different times i.e. 0. hour, 2. hour, and 4. hour. All samples were centrifuged at 0., 2., and 4. hours, and glucose values were measured. No statistically meaningful difference was found between their glucose values in only VACUETTE FC Mix tubes (p>0.05). However, a statistically meaningful difference was found at these different time intervals in serum and fluoride tubes (p<0.01). It was shown that when the blood sampled into VACUETTE FC Mix was kept without centrifugation even at the 4. hour, their glucose values did not decrease and therefore the routine use of them would be beneficial. However, it was found that the glucose values were not stable in VACUETTE CAT Serum Separator and VACUETTE FE.

Keywords

Pregnancy, Gestational Diabetes, Glucose, Glycolysis, Oral Glucose Tolerance Test

Thanks

We are thankful to all the volunteers, who politely donated their blood for the experimental study. In addition, we thank Greiner Bio-One Turkey for supplying the blood collection tubes and accessories free of cost. We also acknowledge the following co-worker for her attendance in the multicenter study: Seda Çağlar.

OGTT Yapılan Gebelerde Kan Örnek Tüplerinin Gestasyonel Diyabet Tanısına Etkisi

Abstract

Gebelikte diagnostik oral glukoz tolerans testi (OGTT) yapılması gestasyonel diyabetin erken tanısı için önemlidir. Hiperglisemi hızlı bir şekilde saptanabilir, böylece anne ve fetüs korunabilir. Bu çalışmada OGTT uygulanan gebelerde kan örneği tüpleri ile glukoz stabilitesi arasındaki ilişkinin araştırılması amaçlanmıştır.Yöntemler: OGTT (75g) yapılan 20 gönüllü gebenin glukoz yüklemesi sonrası 120. dakikada kan örnekleri alındı. Serum için VACUETTE CAT Serum Separator (Clot Activator Tube) kullanıldı; Plazma için VACUETTE FC Mix tüpü (Na2 EDTA, sodyum florür, sodyum sitrat) ve VACUETTE FE (Sodyum Florür/K3 EDTA) kullanıldı. Bu tüplerin üçü de 0. saat, 2. saat ve 4. saat olmak üzere üç farklı zamanda santrifüjlendi. Tüm örnekler 0., 2., 4. saatlerde santrifüj edilerek glukoz değerleri ölçüldü. Sadece VACUETTE FC Mix tüplerinde glukoz değerleri arasında istatistiksel olarak anlamlı fark bulunmadı (p>0.05). Ancak, bu farklı zaman aralıklarında serum ve florürlü tüplerde istatistiksel olarak anlamlı fark bulundu (p<0.01). VACUETTE FC Mix'e alınan kanların 4. saatte bile santrifüj edilmeden bekletildiğinde glukoz değerlerinin düşmediği ve bu nedenle rutin kullanımının faydalı olacağı gösterildi. Ancak VACUETTE CAT serum ayırıcı ve VACUETTE FE'de glukoz değerlerinin stabil olmadığı saptanmıştır.

Keywords

Gebelik, Gestasyonel Diyabet, Glikolizis, Glukoz, Oral Glukoz Tolerans Testi

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