An Investigation of TRIM36 Expression in Breast Cancer

Year 2025, Volume: 12 Issue: 1, 21 - 26, 30.04.2025

Aycan Çelik , Sevim Karakaş Çelik , Güldeniz Karadeniz Çakmak , Fürüzan Köktürk

https://doi.org/10.47572/muskutd.1600240

Abstract

Breast cancer is the most frequently diagnosed cancer type worldwide and ranks 2nd among cancers that cause death. While the role of TRIM36 has been investigated in certain human cancers, such as prostate and lung cancer, its functions remain largely unexplored. In this study, the expression level of TRIM36, whose role in breast cancer has not been previously examined, and its potential association with breast cancer pathogenesis were analyzed. RNA was isolated from normal and tumor tissues of 45 breast cancer patients. Complementary DNA (cDNA) was synthesized from the RNA, and mRNA expression levels of GAPDH and TRIM36 were quantified using qRT-PCR. TRIM36 expression was observed in breast tissues; however, no statistically significant difference was found in TRIM36 mRNA expression levels, normalized to GAPDH, between normal and tumor tissues (p=0.731). Although this study suggests that TRIM36 gene expression levels in breast cancer tissues do not differ from normal tissues, the limited sample size highlights the need for more comprehensive studies to elucidate the relationship between the TRIM36 gene and breast cancer.

Keywords

Gene Expression, Breast Cancer, TRIM36

Ethical Statement

The study was approved by the Zonguldak Bülent Ecevit University Non-Interventional Clinical Research Ethics Committee. (Zonguldak / February 10, 2021/Approval No:2021/03).

Supporting Institution

Zonguldak Bülent Ecevit University

Project Number

2021-50737594-03

Thanks

We thank Zonguldak Bülent Ecevit University Scientific Research Projects Unit for financial support of this study (Project no: 2021-50737594-03). We sincerely thank all the patients who participated in this study for their valuable contributions

Meme Kanserinde TRIM36 Ekspresyonunun İncelenmesi

Abstract

Meme kanseri, dünya genelinde en sık teşhis edilen kanser türü olup, kansere bağlı ölümler arasında ikinci sırada yer almaktadır. TRIM36’nın rolü prostat ve akciğer kanseri gibi bazı insan kanserlerinde araştırılmış olsa da bu proteinin işlevleri büyük ölçüde bilinmemektedir. Bu çalışmada, daha önce meme kanseri ile ilişkisi incelenmemiş olan TRIM36’nın ifade düzeyi ve meme kanseri patogeneziyle potansiyel ilişkisi analiz edilmiştir. Çalışma kapsamında, 45 meme kanseri hastasının normal ve tümör dokularından RNA izole edilmiştir. İzole edilen RNA'dan tamamlayıcı DNA (cDNA) sentezlenmiş ve GAPDH ile TRIM36’nın mRNA ifade düzeyleri qRT-PCR yöntemiyle ölçülmüştür. TRIM36 ifadesinin meme dokularında gözlendiği tespit edilmiştir; ancak, GAPDH’ye normalize edilen TRIM36 mRNA ifade düzeyleri açısından normal ve tümör dokuları arasında istatistiksel olarak anlamlı bir fark bulunamamıştır (p=0.731). Bu çalışma, meme kanseri dokularında TRIM36 gen ifadesinin normal dokulardan farklı olmadığını öne sürse de sınırlı örneklem boyutu, TRIM36 geni ile meme kanseri arasındaki ilişkinin daha kapsamlı çalışmalarla aydınlatılması gerekliliğini ortaya koymaktadır.

Keywords

Gen Ekspresyonu, Meme Kanseri, TRIM36

Project Number

2021-50737594-03

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