Year 2018, Volume 13 , Issue 2, Pages 87 - 93 2018-04-21

DETERMINATION OF ANTIOXIDANT ACTIVITY AND PHENOLIC COMPOUND CONTENT OF BLACK CARROT CALLUS CULTURE
SİYAH HAVUÇ KALLUS KÜLTÜRÜNÜN ANTİOKSİDAN AKTİVİTESİ VE FENOLİK BİLEŞİK İÇERİĞİNİN BELİRLENMESİ

İlhami KARATAŞ [1] , Fatih Polat [2] , Rahime Karataş [3] , Tarık Dal [4] , Mahfuz Elmastaş [5]


Callus culture, a method of plant tissue culture, is an alternative technique for the production of phenolic compounds that many industries need. In this study, antioxidant capacity, total phenolic, flavonoid and anthocyanin content of callus obtained from black carrot (Daucus carota ssp. Sativus var. Atrorubens alef.) were investigated. The antioxidant capacities of the extracts were determined by the free radical scavenging activity (DPPH) and the ferric reducing antioxidant power (FRAP) methods. The callus was derived from the MS medium (Murashige ve Skoog containing 2 mg/L 2.4-D, 0.2mg/L BAP, 30g/L sucrose and 2g/L phytagel using hypocotyl segments of plants grown in sterile conditions. The black carrot calli exhibited high DPPH free radical scavenging activity and FRAP activity in addition to high phenolic compound contents.

Kallus kültürü bir bitki doku kültürü yöntemi olup birçok sektörün ihtiyacı olan fenolik bileşiklerin üretimi için alternatif bir tekniktir. Bu çalışmada, siyah havuç (Daucus carota ssp. sativus var. atrorubens Alef.) bitkisinde kallus kültürü oluşturulması ve elde edilen kallusların antioksidan kapasitesi, toplam fenolik, flavonoid ve antosiyanin içeriklerinin belirlenmesi amaçlanmıştır. Ekstraktların antioksidan kapasiteleri serbest radikal giderme aktivitesi (DPPH: 2.2-diphenyl-1- picrylhydrazyl) ve demir indirgeme antioksidan gücü (FRAP) metotlarıyla
belirlenmiştir. Kallus indüksiyonu, steril koşullarda yetiştirilen bitkilerin hipokotil kısımlarının 2mg/L 2.4-D, 0.2mg/L BAP, 30g/L sükroz ve 2g/L phytagel içeren MS besi ortamında kültüre alınmasıyla (Murashige ve Skoog) sağlanmıştır. Siyah havuç kalluslarının yüksek miktarda fenolik bileşik içermesini yanında yüksek serbest radikal giderme aktivitesi (DPPH) ve demir indirgeme gücüne sahip olduğu belirlenmiştir. 

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Primary Language en
Subjects Science
Journal Section Articles
Authors

Author: İlhami KARATAŞ
Institution: GAZİOSMANPAŞA ÜNİVERSİTESİ
Country: Turkey


Author: Fatih Polat
Institution: GAZİOSMANPAŞA ÜNİVERSİTESİ
Country: Turkey


Author: Rahime Karataş
Institution: Middle Black Sea Transitional Zone Agricultural Research Institute
Country: Turkey


Author: Tarık Dal
Institution: GAZİOSMANPAŞA ÜNİVERSİTESİ
Country: Turkey


Author: Mahfuz Elmastaş
Institution: University of Health Sciences
Country: Turkey


Dates

Publication Date : April 21, 2018

Bibtex @research article { nwsaecolife349702, journal = {Ecological Life Sciences}, issn = {}, eissn = {1308-7258}, address = {}, publisher = {NWSA}, year = {2018}, volume = {13}, pages = {87 - 93}, doi = {}, title = {DETERMINATION OF ANTIOXIDANT ACTIVITY AND PHENOLIC COMPOUND CONTENT OF BLACK CARROT CALLUS CULTURE}, key = {cite}, author = {Karataş, İlhami and Polat, Fatih and Karataş, Rahime and Dal, Tarık and Elmastaş, Mahfuz} }
APA Karataş, İ , Polat, F , Karataş, R , Dal, T , Elmastaş, M . (2018). DETERMINATION OF ANTIOXIDANT ACTIVITY AND PHENOLIC COMPOUND CONTENT OF BLACK CARROT CALLUS CULTURE . Ecological Life Sciences , 13 (2) , 87-93 . Retrieved from https://dergipark.org.tr/en/pub/nwsaecolife/issue/36693/349702
MLA Karataş, İ , Polat, F , Karataş, R , Dal, T , Elmastaş, M . "DETERMINATION OF ANTIOXIDANT ACTIVITY AND PHENOLIC COMPOUND CONTENT OF BLACK CARROT CALLUS CULTURE" . Ecological Life Sciences 13 (2018 ): 87-93 <https://dergipark.org.tr/en/pub/nwsaecolife/issue/36693/349702>
Chicago Karataş, İ , Polat, F , Karataş, R , Dal, T , Elmastaş, M . "DETERMINATION OF ANTIOXIDANT ACTIVITY AND PHENOLIC COMPOUND CONTENT OF BLACK CARROT CALLUS CULTURE". Ecological Life Sciences 13 (2018 ): 87-93
RIS TY - JOUR T1 - DETERMINATION OF ANTIOXIDANT ACTIVITY AND PHENOLIC COMPOUND CONTENT OF BLACK CARROT CALLUS CULTURE AU - İlhami Karataş , Fatih Polat , Rahime Karataş , Tarık Dal , Mahfuz Elmastaş Y1 - 2018 PY - 2018 N1 - DO - T2 - Ecological Life Sciences JF - Journal JO - JOR SP - 87 EP - 93 VL - 13 IS - 2 SN - -1308-7258 M3 - UR - Y2 - 2018 ER -
EndNote %0 Ecological Life Sciences DETERMINATION OF ANTIOXIDANT ACTIVITY AND PHENOLIC COMPOUND CONTENT OF BLACK CARROT CALLUS CULTURE %A İlhami Karataş , Fatih Polat , Rahime Karataş , Tarık Dal , Mahfuz Elmastaş %T DETERMINATION OF ANTIOXIDANT ACTIVITY AND PHENOLIC COMPOUND CONTENT OF BLACK CARROT CALLUS CULTURE %D 2018 %J Ecological Life Sciences %P -1308-7258 %V 13 %N 2 %R %U
ISNAD Karataş, İlhami , Polat, Fatih , Karataş, Rahime , Dal, Tarık , Elmastaş, Mahfuz . "DETERMINATION OF ANTIOXIDANT ACTIVITY AND PHENOLIC COMPOUND CONTENT OF BLACK CARROT CALLUS CULTURE". Ecological Life Sciences 13 / 2 (April 2018): 87-93 .
AMA Karataş İ , Polat F , Karataş R , Dal T , Elmastaş M . DETERMINATION OF ANTIOXIDANT ACTIVITY AND PHENOLIC COMPOUND CONTENT OF BLACK CARROT CALLUS CULTURE. NWSA. 2018; 13(2): 87-93.
Vancouver Karataş İ , Polat F , Karataş R , Dal T , Elmastaş M . DETERMINATION OF ANTIOXIDANT ACTIVITY AND PHENOLIC COMPOUND CONTENT OF BLACK CARROT CALLUS CULTURE. Ecological Life Sciences. 2018; 13(2): 87-93.
IEEE İ. Karataş , F. Polat , R. Karataş , T. Dal and M. Elmastaş , "DETERMINATION OF ANTIOXIDANT ACTIVITY AND PHENOLIC COMPOUND CONTENT OF BLACK CARROT CALLUS CULTURE", Ecological Life Sciences, vol. 13, no. 2, pp. 87-93, Apr. 2018