YABAN MERSİNİ (Blue berry) VE KURT ÜZÜMÜ (Gojiberry) EKSTRAKTLARIYLA ZENGİNLEŞTİRİLMİŞ KİTOSAN ile KAPLANMIŞ GÖKKUŞAĞI ALABALIK (Onchorhynchus mykiss WALBAUM 1792) FİLETOLARININ PİYASA KOŞULLARINDA MİKROBİYOLOJİK DEĞİŞİMLERİNİN İNCELENMESİ

Year 2018, Volume: 13 Issue: 4, 171 - 181, 14.10.2018

Ayşe Gürel İnanlı Özlem Emir Çoban Songül Yüce Burcu Çelik

Abstract

Çalışma, yaban mersini
ve kurt üzümü ekstraktları ilaveli kitosan kaplamanın, gökkuşağı alabalığı
filetolarında mikrobiyel gelişmeyi önleyebilmek ya da kontrol altına alabilmek
için ne kadar etkili olduğunu belirlemek amacıyla yapılmıştır. Filetolar, normal,
kitosan kaplı, yaban mersini ekstraktı ilaveli kitosan kaplı, kurt üzümü
ekstraktı ilaveli kitosan kaplı olmak üzere toplam 4 deneysel grup olarak
strafor paketler içerisinde +8 °C’de muhafazaya alınmıştır. Muhafazadaki
örneklerin 3 günde bir mezofil aerob bakteri, psikrofil aerob bakteri, maya-küf
ve Enterobakteriaceae bakteri
sayımları yapılmıştır. Çalışmada belirlenen TMAB sayısı tüm gruplarda ise 6.
günde kabul edilebilir limit değerini aşmıştır. TPAB sayıları tüm gruplarda
muhafaza süresince zamana bağlı olarak artmıştır. Depolama sonunda maya-küf
sayıları, en yüksek kontrol grubunda 5,14±0,12 log kob/g, en düşük kurt üzümü
ilaveli kitosanla kaplanmış grubta 3,74±0,19 log kob/g olarak belirlenmiştir. Çalışmadaki
örneklerde Enterobacteriaceae bakteri
sayısı en yüksek 6. günde kontrol grubunda (5,28±0,22 log kob/g) saptanmıştır. Sonuç
olarak; kitosan ve bikki ekstaktı ilaveli kitosan kaplamaların filetoların
mikrobiyolojik nitelikleri üzerinde olumlu etkisinin olduğu kanaatine
varılmıştır. 

Keywords

Kitosan, Oncorhynchus mykiss, Yaban Mersini, Kurt Üzümü, Mikrobiyolojik Değişim

References

• • Alak, G., Hisar, Ş.A., Hisar, O., Kaban, G., and Kaya, M., (2010). Microbiological and Chemical Properties of Bonito Fish (Sarda sarda) Fillets Packaged with Chitosan Film, Modified Atmosphere and Vacuum. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. Cilt:16, ss:73-80.
• • Arashisar, Ş., Hisar, O., Kabani, G., Kayal, M., and Alak G., (2009). Effect of Chitosan Coating on Chemical And Microbiological Properties of Atlantic Bonito (Sarda sarda) Fillets. Journal of Food Science Technology, Volume:46, Issue:4, pp:380-383.
• • Bakkali, F., Averbeck, S., Averbeck, D., and Idaomar, M., (2008). Biological Effects of Essential Oils. A Review, Food and Chemical Toxicology, Volume:46, pp:446–475.
• • Becerril, R, Gomez-Lus, R., Goni, P., Lopez, P., and Nerin, C., (2007). Combination of Analytical and Microbiological Techniques to Study The Antimicrobial Activity of A New Active Food Packaging Containing Cinnamon or Oregano Against E. coli and S. aureus. Anal Bioanal Chemistry, Volume:388, pp:1003-1011.
• • Cabrita, L., Fruystein, N.A., and Andersen, Q.M., (2000). Anthocyanin Trisaccharides in Blueberries of Vaccinium Padifolium, Food Chemistry, Volume:69 pp:33-36.
• • Ceylan, A., (1983). Tıbbi Bitkiler-II. Ege Üniversitesi Ziraat Fakültesi Yayını No:481, Bornova-İzmir.
• • Chien, P.J., Sheu, F., and Yang, F.H., (2007). Effects of Edible Chitosan Coating on Quality and Shelf Life of Sliced Mango Fruit. Journal of Food Engineering, Volume:78, pp:225–229
• • Christie, W.W., (1990). Gas Chromatography and Lipids, The Oil Press, Glaskow, pp:302.
• • Çelik，H., (2005). Yaban Mersini (Likapa) Yetiştiriciliği. HASAD Yayın, S:128.
• • Davidson, P.M., Post, L.S., Branen, A.L., and Mccurdy, A.R., (1983). Naturally Occurring and Miscellaneous Food Antimicrobials. In Antimicrobials in Food, pp:371-419.
• • Devlieghere, F., Vermeulen, A., and Debevere, J., (2004). Chitosan: Antimicrobial Activity, İnteractions with Food Components and Applicability as a Coating on Fruit and Vegetables, Food Microbiology, Volume:21, pp:703–714.
• • Du, J., Gemma, H., and Ivahori S., (1997). Effect of Chitosan Coating on Storage of Peach, Japanese Pear and Kiwifruit, Journal of the Japanese Society for Horticultural Science, Volume:66, Issue:1, pp:15-22.
• • Duan, J., Cherian, G., and Zhao, Y., (2010a). Quality Enhancement in Fresh and Frozen Lingcod (Ophiodon elongates) Fillets by Employment of Fish oil Incorporated Chitosan Coatings, Food Chemistry, Volume:119, pp:524-532.
• • Duan, J., Jiang, Y., Cherian, G., and Zhao, Y., (2010b). Effect of Combined Chitosan-Krill Oil Coating and Modified Atmosphere Packaging on The Storability of Cold-Stored Lingcod (Ophiodon elongates) Fillets, Food Chemistry, Volume:122, Issue:4, pp:1035–1042
• • Dursun, S. and Erkan, N., (2009). Yenilebilir Protein Filmler ve Su Ürünlerinde Kullanımı, Journal of Fisheries Sciences.com, Volume:3, Issue:4, pp:352-373
• • Fan, W., Sun, J., Chen, Y., Qiu, J., Zhang, Y., and Chi, Y., (2009). Effects of Chitosan Coating on Quality and Shelf Life of Silver Carp During Frozen Storage, Food Chemistry, Volume:115, pp:66–70
• • Ghaouth, A.E., Ponnampalam, R., Castaigne, F., and Ar, J., (1992). Chitosan Coating to Extend the Storage Life of Tomatoes, Hortscience, Volume:27, Issue:9, pp:1016-1018.
• • Gomez-Estaca, J., Lopez De Lacey, A., Lopez-Caballero, M.E., Gomez-Guillen, M.C., and Montero, P., (2010). Biodegradable Gelatinechitosan Films Incorporated with Essential Oils as Antimicrobial Agents for Fish Preservation. Food Microbiology. 27, 889-896.
• • Gomez-Estaca, J., Montero, P., Gime’nez, B., and Go’mez-Guille’n, M.C., (2007). Effect of Functional Edible Films and High Pressure Processing on Microbial and Oxidative Spoilage in Cold-Smoked Sardine (Sardina pilchardus). Food Chemistry, Volume:105, pp:511–520.
• • Gutierrez, L., Sanchez, C., Batlle, R., and Nerın, C., (2009). New Antimicrobial Active Package for Bakery Products, Trends in Food Science and Technology, Volume:20, pp:92-96.
• • Günlü, A. and Koyun, E., (2012). Effects of Vacuum Packaging and Wrapping with Chitosan-Based Edible Film on the Extension of the Shelf Life of Sea Bass (Dicentrarchus labrax) Fillets in Cold Storage (4°C), Food and Bioprocess Technology. DOİ:10.1007/11947-012-0833-6.
• • Halkman, A.K., (2005). Merck Gıda Mikrobiyolojisi Uygulamaları, Başak Matbaacılık, Ankara.
• • Han, C., Lederer, C., Mcdanıel, M., and Zhao, Y., (2005). Sensory Evaluation of Fresh Strawberries (Fragaria ananassa) Coated with Chitosan-based Edible Coatings. Journal of Food Scıence, Volume:70, Issue:3, pp:172-178.
• • Harrigan, W.F. and Mccance, M.E., (1976). Laboratory Metods in Food and Dairy Microbiology. Academic Press, London, pp:362.
• • Harrigan, W.F., (1998). Laboratory Methods in Food Microbiology, 3rd ed.Academic Press., London.
• • ICMSF, (1986). Microorganisms in Foods. Sampling for Microbiological Analysis: Principles and Scientific Applications, Toronto: University of Toronto Press., pp:181–196.
• • Jeon, Yj., Kamil, Jyva., and Shahidi, F., (2002). Chitosan as an Edible Invisible Film for Quality Preservation of Herring and Atlantic Cod, Journal of Agricultural and Food, Volume:20, Issue:50, pp:5167–5178 http://dx.doi.org/10.1021/jf011693l.
• • Kammerer, D.R., Schillmöller, S., Maier, O., Schieber, A., and Carle, R., (2007). Colour Stability of Canned Strawberries using Black Carrot and Elderberry Juice Concentrates as Natural Colourants. European Food Research and Technology, Volume:224 Issue:6, pp:667–679.
• • Kılıç, A., (2008). Uçucu Yağ Elde Etme Yöntemleri, Bartın Orman Fakültesi Dergisi 10, 13, 37-45.
• • Lee, J., Durst, R.W., and Wrolstad, R.E., (2002). Impact of Juice Processing on Blueberry Anthocyanins and Polyphenolics: Comparison of Two Pretreatments. Journal of Food Science, Volume:67, Issue:5, pp:1660-1667.
• • Mahan, F.I., (2007). Kitosanla Kaplanmış Soyulmuş Sosislerin Mikrobiyolojik Kalitesi ve Raf Ömürlerinin Araştırılması (Yüksek Lisans Tezi), İstanbul Üniversitesi Sağlık Bilimleri Enstitüsü, Besin Hijyeni ve Teknolojisi Anabilim Dalı.
• • Ojagh, S.M., Rezaei, M., Razavi, S.H., and Hosseini, S.M.H., (2010). Effect of Chitosan Coatings Enriched with Cinnamon Oil on the Quality of Refrigerated Rainbow Trout. Food Chemistry, Volume:120, Issue:1, pp:193–198. http://dx.doi.org/10.1016/j.foodchem.2009.10.006.
• • Özdamar, K., (2001). SPSS ile Biyoistatistik. Yayın no:3,4. Baskı. Kaan Kitabevi, Eskişehir, pp:452.
• • Ponce, A.G., Roura, S.I., Del Valle, C.E., and Moreira, M.R., (2008). Antimicrobial and Antioxidant Activities of Edible Coatings Enriched with Natural Plant Extracts: In vitro and in vivo studies, Postharvest Biology and Technology, Volume:49, pp:294-300.
• • Quattara, B., Simard, Re., Piette, G., Begin, A., and Holley R., (2000). Inhibition of Surface Spoilage Bacteria in Processed Meats by Application of Antimicrobial Films Prepared with Chitosan, International Journal of Food Microbiology, Volume:62, pp:139-148.
• • Runfeng, W. and Li, Z., (2011). Effect of Chitosan on Fresh-Keeping Refrigerated fillets of Fresh Ctenopharyngodon Idellus. IEEE, pp:1061-1064.
• • Sallam, K.I., (2007). Antimicrobial and Antioxidant Effects of Sodium Acetate, Sodium Lactate, and Sodium Citrate in Refrigerated Sliced Salmon. Food Control, Volume:18, Issue:5, pp:566–575.
• • Sedigh-Jasour, M., Ehsani, A., Mehryar, L., and Naghibis, S., (2015). Chitosan Coating Incorporated with the Lactoperoxidase System: An Active Edible Coating for Fish Preservation. Journal of the Science of Food and Agriculture. DOI: 10.1002/jsfa.6838
• • Vásconez, M.B., Flores, S.K., Campos, C.A., Alvarado, J., and Gerschenson, L.N., (2009). Antimicrobial Activity and Physical Properties of Chitosan–Tapioca Starch Based Edible Films and Coatings, Food Research International, Volume:42, Issue:7, pp:762–769.
• • Ye, M., Neetoo, H., and Chen, H., (2008), Effectiveness of Chitosan-Coated Plastic Films in Corporating Antimicrobials in İnhibition of Listeria Monocytogenes on Cold-Smoked Salmon, International Journal of Food Microbiology, Volume:127, pp:235–240.
• • Zivanovic, S., Chi, S., and Draughton, A.E., (2005). Antimicrobial Activity of Chitosan Films Enriched with Essential Oils, Journal of Food Science, Volume:70, pp:45-51.