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İki Farklı Aydınlatma Sistemi Altında Yetiştirilen Japon Bıldırcınların But Kaslarındaki MyoD, IGF-I ve STAT5B Genlerin İfadesinin Belirlenmesi

Year 2022, Volume: 5 Issue: 2, 792 - 799, 18.07.2022
https://doi.org/10.47495/okufbed.997922

Abstract

Işık büyüme ve üremede oldukça önemli bir faktördür. Bu çalışma, Japon bıldırcınlarının but kaslarında MyoD, IGF-I ve STAT5B genlerinin ekspresyonu üzerine iki farklı aydınlatma sisteminin etkisini belirlemeyi amaçlamaktadır. Bu amaçla iki aydınlatma sistemi kullanılmıştır; 16 saat aydınlık: 8 saat karanlık (16 saat U: 8 saat G) grup I ve 8 saat aydınlık: 16 saat karanlık (8 saat U: 16 saat G) grup II. But kaslarındaki gen ekspresyon seviyelerinin değerlendirilmesi için kantitatif real-time PCR kullanılmıştır. Real Time PCR sonuçları değerlendirildiğinde grup I'de grup II'ye göre MyoD gen ekspresyonunun daha yüksek olduğu belirlenmiştir. Öte yandan IGF-I ve STAT5B gen ekspresyonları değerlendirildiğinde ise tam tersi durum yani grup II'de grup I'e göre daha yüksek ekspresyon saptanmıştır.

References

  • Reference1 [1] Mohammed H., Grashorn M., Bessei W. The effects of lighting conditions on the behaviour of laying hens, Archiv für Geflügelkunde 2010; 74(3): 197-202. Reference2 [2] Retes P.L., Espósito M., das Neves D.G., Viana A.G., Coelho L.M., Bobadilla-Mendez M.F., Alvarenga R.R., Fassani E.J., Peixoto J.V., Zangeronimo M.G. Influence of different types of lamps on the reproductive development of male japanese quail (coturnix coturnix japonica), Theriogenology 2017; 94(59-63.

Determining Gene Expression of MyoD, IGF-I and STAT5B Genes in the Japanese Quail Leg Muscles Which Reared Under Two Different Lighting Systems

Year 2022, Volume: 5 Issue: 2, 792 - 799, 18.07.2022
https://doi.org/10.47495/okufbed.997922

Abstract

Light is a very important factor in growth and reproduction. This study aims to determine the effect of two different lighting systems on the expression of MyoD, IGF-I and STAT5B genes in the leg muscles of the Japanese quail. For this aim, two lighting systems were used; 16 hours’ light: 8 hours’ darkness (16 h L: 8 h D) group I and 8 hours’ light: 16 hours’ darkness (8 h L: 16 h D) group II. Quantitative real-time PCR was used for assessment of the gene expression levels in leg muscles. When the Real Time PCR results were evaluated, it was determined that MyoD gene expression was higher in group I than in group II.
On the other hand, when IGF-I and STAT5B gene expressions were evaluated, the opposite situation was found, namely higher expression in group II compared to group I.

References

  • Reference1 [1] Mohammed H., Grashorn M., Bessei W. The effects of lighting conditions on the behaviour of laying hens, Archiv für Geflügelkunde 2010; 74(3): 197-202. Reference2 [2] Retes P.L., Espósito M., das Neves D.G., Viana A.G., Coelho L.M., Bobadilla-Mendez M.F., Alvarenga R.R., Fassani E.J., Peixoto J.V., Zangeronimo M.G. Influence of different types of lamps on the reproductive development of male japanese quail (coturnix coturnix japonica), Theriogenology 2017; 94(59-63.
There are 1 citations in total.

Details

Primary Language English
Subjects Zootechny (Other)
Journal Section RESEARCH ARTICLES
Authors

Mervan Bayraktar 0000-0003-3268-864X

Ela Nur Şimşek Sezer

Publication Date July 18, 2022
Submission Date September 20, 2021
Acceptance Date December 17, 2021
Published in Issue Year 2022 Volume: 5 Issue: 2

Cite

APA Bayraktar, M., & Şimşek Sezer, E. N. (2022). Determining Gene Expression of MyoD, IGF-I and STAT5B Genes in the Japanese Quail Leg Muscles Which Reared Under Two Different Lighting Systems. Osmaniye Korkut Ata Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 5(2), 792-799. https://doi.org/10.47495/okufbed.997922
AMA Bayraktar M, Şimşek Sezer EN. Determining Gene Expression of MyoD, IGF-I and STAT5B Genes in the Japanese Quail Leg Muscles Which Reared Under Two Different Lighting Systems. Osmaniye Korkut Ata University Journal of The Institute of Science and Techno. July 2022;5(2):792-799. doi:10.47495/okufbed.997922
Chicago Bayraktar, Mervan, and Ela Nur Şimşek Sezer. “Determining Gene Expression of MyoD, IGF-I and STAT5B Genes in the Japanese Quail Leg Muscles Which Reared Under Two Different Lighting Systems”. Osmaniye Korkut Ata Üniversitesi Fen Bilimleri Enstitüsü Dergisi 5, no. 2 (July 2022): 792-99. https://doi.org/10.47495/okufbed.997922.
EndNote Bayraktar M, Şimşek Sezer EN (July 1, 2022) Determining Gene Expression of MyoD, IGF-I and STAT5B Genes in the Japanese Quail Leg Muscles Which Reared Under Two Different Lighting Systems. Osmaniye Korkut Ata Üniversitesi Fen Bilimleri Enstitüsü Dergisi 5 2 792–799.
IEEE M. Bayraktar and E. N. Şimşek Sezer, “Determining Gene Expression of MyoD, IGF-I and STAT5B Genes in the Japanese Quail Leg Muscles Which Reared Under Two Different Lighting Systems”, Osmaniye Korkut Ata University Journal of The Institute of Science and Techno, vol. 5, no. 2, pp. 792–799, 2022, doi: 10.47495/okufbed.997922.
ISNAD Bayraktar, Mervan - Şimşek Sezer, Ela Nur. “Determining Gene Expression of MyoD, IGF-I and STAT5B Genes in the Japanese Quail Leg Muscles Which Reared Under Two Different Lighting Systems”. Osmaniye Korkut Ata Üniversitesi Fen Bilimleri Enstitüsü Dergisi 5/2 (July 2022), 792-799. https://doi.org/10.47495/okufbed.997922.
JAMA Bayraktar M, Şimşek Sezer EN. Determining Gene Expression of MyoD, IGF-I and STAT5B Genes in the Japanese Quail Leg Muscles Which Reared Under Two Different Lighting Systems. Osmaniye Korkut Ata University Journal of The Institute of Science and Techno. 2022;5:792–799.
MLA Bayraktar, Mervan and Ela Nur Şimşek Sezer. “Determining Gene Expression of MyoD, IGF-I and STAT5B Genes in the Japanese Quail Leg Muscles Which Reared Under Two Different Lighting Systems”. Osmaniye Korkut Ata Üniversitesi Fen Bilimleri Enstitüsü Dergisi, vol. 5, no. 2, 2022, pp. 792-9, doi:10.47495/okufbed.997922.
Vancouver Bayraktar M, Şimşek Sezer EN. Determining Gene Expression of MyoD, IGF-I and STAT5B Genes in the Japanese Quail Leg Muscles Which Reared Under Two Different Lighting Systems. Osmaniye Korkut Ata University Journal of The Institute of Science and Techno. 2022;5(2):792-9.

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