Determining WRKY transcription factors related to salinity stress response in wheat (Triticum aestivum L.) and barley (Hordeum vulgare L.)

Year 2020, Volume: 35 Issue: 1, 1 - 7, 14.02.2020

Feyza Tufan

https://doi.org/10.7161/omuanajas.527704

Cited By: 3

Abstract

The salinity is one of most common stress
factors with devastating effects for wheat (Triticum aestivum L.) and barley (Hordeum vulgare L). The
aim of this study was to determine the salt stress response associated with WRKY
transcription factors by gene expression analysis. Seeds of T. aestivum L. cvs.
Esperia and Bezozya, and H. vulgare L. cvs. Lord and Ramata were subjected to salt stress
for 10 days with two concentrations of NaCl (1.0 % and 2.0 %). No germination
was observed in 2.0 % NaCl treated seeds. Nearly all the WRKY genes studied were
upregulated and downregulated in response to 1.0 % NaCl stress in relatively
resistant and sensitive cultivars in comparison to control sets, respectively.
Among the screened genes, the expression of TaWRKY7, 40, 41, 53,
68, 72 and 79 genes were increased in relatively resistant
Esperia cultivar in wheat. Similarly, 7 of WRKY genes (HvWRKY6, 9, 24, 25,
33, 34, 42) were upregulated in relatively resistant Lord
cultivar in barley. In terms of WRKY gene expression profile, since TaWRKY7 and TaWRK72 in wheat and HvWRKY33 in
barley increased significantly, these genes can be used as marker genes for
further investigation of abiotic stress response. This study is a preliminary
study in terms of providing an association between WRKY genes and salinity stress response
of wheat and barley breeding cultivars in Turkey.

Keywords

Gene expression, WRKY, Salt stress, Hordeum vulgare L., Triticum aestivum L.

Buğday (Triticum aestivum L.) ve arpada (Hordeum vulgare L.) tuzluluk stres yanıtı ile ilişkili WRKY transkripsiyon faktörlerinin belirlenmesi

Abstract

Tuzluluk, buğday (Triticum
aestivum L.) ve arpa (Hordeum
vulgare L.) için yıkıcı
etkileri olan en yaygın stres faktörlerinden biridir. Bu çalışmada, WRKY transkripsiyon faktörleriyle ilişkili tuz
stresi yanıtının gen ekspresyon analizi ile belirlenmesi amaçlandı. T.
aestivum L. cvs. Esperia ve
Bezozya ile H. vulgare L.
cvs. Lord ve Ramata tohumlarına iki NaCl konsantrasyonu (% 1.0 ve % 2.0) ile 10
gün boyunca tuz stresi uygulandı. % 2.0 NaCl uygulanan tohumlarda çimlenme
gözlenmedi. Çalışılan hemen tüm WRKY genleri, kontrol gruplarına kıyasla % 1.0 NaCl stresine yanıt olarak
nispeten dirençli ve hassas kültivarlarda sırasıyla yukarı ve aşağı yönde
düzenlendi. İncelenen genler arasında, buğdayda nispeten dirençli Esperia
kültivarında TaWRKY7, 40, 41, 53, 68, 72 ve 79
genlerinin ekspresyonu indüklendi. Benzer şekilde, arpada nispeten dirençli
Lord’da 7 WRKY geni (HvWRKY6, 9, 24, 25, 33, 34,
42) indüklendi. WRKY gen ekspresyon profili açısından, buğdayda TaWRKY7 ve TaWRK72 ile arpada HvWRKY33 genlerinde anlamlı derecede artış saptanması nedeniyle bu genler abiyotik
stres yanıtının daha fazla araştırılması için marker genler olarak
kullanılabilir. Bu çalışma, Türkiye’de ekimi yapılan buğday ve arpa
çeşitlerinde WRKY genleri ve tuz stresi yanıtı arasında bir ilişki sağlaması açısından öncül
bir çalışma niteliğindedir.          

Keywords

Gen ekspresyonu, WRKY, Tuz stresi, Hordeum vulgare L., Triticum aestivum L.

References

• Aktaş, Y.L. and Güven, A., 2005. Bitki savunma sistemlerinde hormonal sinyal moleküler ve çapraz iletişimleri. J. Arts Sci., 3:1-12.
• Almodares, A.M., Hadi, R., Kholdebarin, B., Samedani, B. and Kharazian, A., 2014. The response of sweet sorghum cultivars to salt stress and accumulation of Na+, Cl- and K+ ions in relation to salinity. J. Environ. Biol., 35:733–739. Athar, H.R. and Ashraf, M., 2009. Strategies for crop improvement against salinity and drought stress: An overview. TAVS, 44:1–16.
• Badridze, G., Weidner, A. and Asch, F., 2009. Variation in salt tolerance within a Georgian wheat. Genet. Resour. Crop Evol., 56:1125–1130. Bagdi, D.L. and Shaw, B.P., 2013. Analysis of proline metabolic enzymes in Oryza sativa under NaCl stress. J. Environ. Biol., 34:677–681.
• Bagdi, D.L., Shaw, B.P., Sahu, B.B. and Purohit, G.K., 2015. Real time PCR expression analysis of gene encoding p5cs enzyme and proline metabolism under NaCl salinity in rice. J. Environ. Biol., 36:955–961.
• Brenchley, R., Spannagl, M., Pfeifer, M., Barker, G.L., D’Amore, R., Allen, A.M. et al., 2012. Analysis of the bread wheat genome using whole-genome shotgun sequencing. Nature, 491:705–710.
• Budak, H., Hussain, B., Khan, Z., Ozturk, N.Z. and Ullah, N., 2015. From genetics to functional genomics: improvement in drought signaling and tolerance in wheat. Front. Plant. Sci., 6:1012.
• Eulgem, T., Rushton, P.J., Robatzek, S. and Somssich, I.E., 2009. The WRKY superfamily of plant transcription factors. Trends Plant Sci., 5:199–206.
• Fowler, S. and Thomashow, M.F., 2002. Arabidopsis transcriptome profiling indicates that multiple regulatory pathways are activated during cold acclimation in addition to the CBF cold response pathway. Plant Cell, 14:1675–1690.
• Hu, Y. and Schmidhalter, U., 2005. Drought and salinity: A comparison of their effects on mineral nutrition of plants. J. Plant Nutr. Soil Sci., 168:541–549.
• Imadi, S.R,, Kazi, A.G., Ahanger, M.A., Gucel, S., Ahmad, P., 2015. Plant transcriptomics and responses to environmental stress: an overview. J. Genet., 94:525–537.
• Jain, R., Singh, S.P., Singh, A., Singh, S., Tripathi, P., Chandra, A. et al., 2016. Study on physio-biochemical attributes and metallothionein gene expression affected by chromium (VI) in sugarcane (Saccharum spp. hybrid). J. Environ. Biol., 37:375–382.
• Marè, C., Mazzucotelli, E., Crosatti, C., Francia, E., Stanca, A.M. and Cattivelli, L., 2004. HvWRKY38: A new transcription factor involed in cold- and drought- response in barley. Plant Mol. Biol., 55:309–416.
• Niu, C.F., Wei, W., Zhou, Q.Y., Tian, A.G., Hao, Y.J., Zhang, W.K. et al., 2012. Wheat WRKY genes TaWRKY2 and TaWRKY19 regulate abiotic stress tolerance in transgenic Arabidopsis plants. Plant Cell Environ., 35:1156–1170.
• Panda, S.K., Chaudhury, I. and Khan, M.H., 2003. Heavy metals induce lipid peroxidation and affect antioxidants in wheat leaves. Biol. Plant., 46:289–294.
• Robatzek, S. and Somssich, I.E., 2002. Targets of AtWRKY6 regulation during plant senescence and pathogen defense. Genes Dev, 16:1139–1149. Rushton, P.J., Somssich, I.E., Ringler, P. and Shen, Q.J., 2010. WRKY transcription factors, Trends. Plant. Sci., 15:247–258.
• Singh, K.K., Das, M.M., Samanta, A.K., Kundu, S.S. and Sharma, S.D., 2002. Evaluation of certain feed resources for carbohydrate and protein fractions and in situ digestion characteristics. Indian J. Anim. Sci., 72:794–797.
• Wang, X., Zeng, J., Li, Y., Rong, X., Sun, J., Sun, T. et al., 2015. Expression of TaWRKY44, a wheat WRKY gene, in transgenic tobacco confers multiple abiotic stress tolerances. Front Plant Sci., 6:615.
• Yadav, S., Irfan, M., Ahmad, A. and Hayat, S., 2011. Causes of salinity and plant manifestations to salt stress A review. J. Environ Biol., 32:667–685.
• Yörük, E., Keleş, E.N., Sefer, Ö. and Eraslan, M., 2018. Salinity and drought stress investigations on barley (Hordeum vulgare L.) and wheat (Triticum aestivum L.) cultivars planted in Turkey. J. Environ. Biol., 39:943-950.