Research Article
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Year 2021, Volume 38, Issue 3, 272 - 276, 01.05.2021

Abstract

References

  • Chen, W., Hu, J., Zhang, Z., Chen, L., Xie, H., Dong, N., Chen Y., Zuguo Liu, Z., 2012. Localization and expression of Zonula Occludins-1 in the rabbit corneal epithelium following exposure to benzal konium chloride. PLoS ONE. 7(7): e40893.
  • Chiquoine, A.D.,1964. Observations on the early events of cadmium necrosis of the testis. AnatRec. 149:23–35.
  • Garg, V., Gard, S.P., 2011. Role of nitric oxide in male infertility. J Indian Acad. Forensic Med. 33(1). 65-68.
  • Gao, Y., Mruk, D., Chen, H., Lui, W.Y., Lee, W.M., Cheng, C.Y., 2017.Regulation of the blood-testis barrier by a local axis in the testis: role of laminin α2 in the basement membrane. FASEB J.31(2):584-597.
  • Hikim, A. P., Lue, Y., Yamamoto, C. M., Vera, Y., Rodriguez, S., Yen, P. H., Soeng, K., Wang, C., Swerdloff, R.S., 2003. Key apoptotic pathways for heat-induced programmed germ cell death in the testis. Endocrinology. 144, 3167–3175.
  • Jiang, X.H.,Bukhari, I., Zheng, W., Yin, S., Wang, Z., Cooke H.J., Shi, Q.H., 2014.Blood-testis barrier and spermatogenesis: lessons from genetically-modified mice.Asian J. Androl.16(4):572-80.
  • Kang Z., Qiao N., Liu G., Chen H., Tang Z., Ying Li Y., 2017. Copper-induced apoptosis and autophagy through oxidative stress-mediated mitochondrial dysfunction in male germ cells. Toxicol In Vitro. 61:104639.
  • Kumar, N., Singh, A.K., 2015. Trends of male factor infertility, an important cause of infertility: A review of literature. J. Hum.Reprod Sci. 8(4):191-6.
  • Lee, N.P., Cheng, C.Y., 2008. Nitric oxide and cyclic nucleotides: Their roles ınjunction dynamics and spermatogenesis. Adv. Exp. Med. Biol.636:172-85.
  • Lee, N.P.Y., Cheng, C.Y., 2003. Regulation of sertoli cell tight junction in the rat testis viathe nitric oxide synthase/soluble guanylate cyclase/cGMP/Protein Kinase G signaling pathway: an in vitro study. Endocrinology.144:3114-3129.
  • Nieschlag, E., Behre, H., Nieschlag, S., Van, Ahlen H. 2011. Male reproductive health and dysfunction. Internistische Praxis. Andrology. 51: 751.
  • Rumbold, A.R., Sevoyan, A., Oswald, T.K., Fernandez R.C., Davies, M.J., Moore, V.M., 2019. Impact of male factor infertility on offspring health and development. FertilSteril. 111(6):1047-1053.
  • Sapanidou, V., Taitzoglou, I., Tsakmakidis, I.,Kourtzelis, I., Fletouris, D., Theodoridis, A., Zervos, I., Tsantarliotou, M., 2015. Antioxidant effect of crocin on bovine sperm quality and in vitro fertilization. Theriogenology. 84(8):1273-82.
  • Skakkebaek, N.E., Rajpert De Meyts, E., Main, K.M., 2001. Testicular dysgenesis syndrome: an increasingly common developmental disorder with environmental aspects. Hum.Reprod 16: 972−8.
  • Sun,H.,Yang, B., Zhu, C., Liu, R., Wang, H., Li, W., 2013. Presence of metastasis-associated protein 1 in Sertoli cells is required for propercontact between sertoli cells and adjacent germ cells.Urology.81(1):66-73.
  • Vlajkovic, S., Cukuranovic, R., Bjelakovic, M.R., Stefanovic. V. 2012. Possible Therapeutic Use of Spermatogonial Stem Cells in the Treatment of Male Infertility: A Brief Overview. ScientificWorldJournal. 8.
  • Yan Cheng,C.,Dolores, D.M, 2012.The Blood-Testis Barrier and Its Implications for Male Contraception. PharmacolRev. 64(1): 16–64.
  • WHO.,2010. World Health organization laboratory manual for the examination and the processing of human semen. 5 th ed;pp161.

Ultrastructural examination of testicular tissues and evaluation of ZO-1 protein levels obtained from azoospermic cases

Year 2021, Volume 38, Issue 3, 272 - 276, 01.05.2021

Abstract

Spermatogenesis is a process that involves cellular and biochemical processes. The purpose of this study is to understand the role of tight junctions in spermatogenesis by evaluating Zonula occludens-1 (ZO-1) protein levels of cells from the testicular sperm extraction (TESE) biopsies in patients with azoospermia The study included 40 infertile men with azoospermia. They were divided into two groups, as spermatozoa positive (n=20) and spermatozoa negative (n=20). Testis tissues were examined morphologically, immunohistochemically and ultrastructurally by using transmission electron microscopy (TEM). The structure of seminiferous tubules were deteriorated, degenerated and hyalinized in spermatozoa negative group. ZO-1 expression level was normal in blood-testis barrier of spermatozoa positive group in comparing to lateral surfaces of sertoli cells spermatozoa negative group. The ultrastructural results were also supported with light microscopy findings. These findings suggest that decrease of the ZO-1 protein expression level in Sertoli cells might be one of the factors involved in the impairment of spermatogenesis in spermatozoa negative group. Therefore, we suggest that tight junctions may have crucial roles in spermatogenesis.

References

  • Chen, W., Hu, J., Zhang, Z., Chen, L., Xie, H., Dong, N., Chen Y., Zuguo Liu, Z., 2012. Localization and expression of Zonula Occludins-1 in the rabbit corneal epithelium following exposure to benzal konium chloride. PLoS ONE. 7(7): e40893.
  • Chiquoine, A.D.,1964. Observations on the early events of cadmium necrosis of the testis. AnatRec. 149:23–35.
  • Garg, V., Gard, S.P., 2011. Role of nitric oxide in male infertility. J Indian Acad. Forensic Med. 33(1). 65-68.
  • Gao, Y., Mruk, D., Chen, H., Lui, W.Y., Lee, W.M., Cheng, C.Y., 2017.Regulation of the blood-testis barrier by a local axis in the testis: role of laminin α2 in the basement membrane. FASEB J.31(2):584-597.
  • Hikim, A. P., Lue, Y., Yamamoto, C. M., Vera, Y., Rodriguez, S., Yen, P. H., Soeng, K., Wang, C., Swerdloff, R.S., 2003. Key apoptotic pathways for heat-induced programmed germ cell death in the testis. Endocrinology. 144, 3167–3175.
  • Jiang, X.H.,Bukhari, I., Zheng, W., Yin, S., Wang, Z., Cooke H.J., Shi, Q.H., 2014.Blood-testis barrier and spermatogenesis: lessons from genetically-modified mice.Asian J. Androl.16(4):572-80.
  • Kang Z., Qiao N., Liu G., Chen H., Tang Z., Ying Li Y., 2017. Copper-induced apoptosis and autophagy through oxidative stress-mediated mitochondrial dysfunction in male germ cells. Toxicol In Vitro. 61:104639.
  • Kumar, N., Singh, A.K., 2015. Trends of male factor infertility, an important cause of infertility: A review of literature. J. Hum.Reprod Sci. 8(4):191-6.
  • Lee, N.P., Cheng, C.Y., 2008. Nitric oxide and cyclic nucleotides: Their roles ınjunction dynamics and spermatogenesis. Adv. Exp. Med. Biol.636:172-85.
  • Lee, N.P.Y., Cheng, C.Y., 2003. Regulation of sertoli cell tight junction in the rat testis viathe nitric oxide synthase/soluble guanylate cyclase/cGMP/Protein Kinase G signaling pathway: an in vitro study. Endocrinology.144:3114-3129.
  • Nieschlag, E., Behre, H., Nieschlag, S., Van, Ahlen H. 2011. Male reproductive health and dysfunction. Internistische Praxis. Andrology. 51: 751.
  • Rumbold, A.R., Sevoyan, A., Oswald, T.K., Fernandez R.C., Davies, M.J., Moore, V.M., 2019. Impact of male factor infertility on offspring health and development. FertilSteril. 111(6):1047-1053.
  • Sapanidou, V., Taitzoglou, I., Tsakmakidis, I.,Kourtzelis, I., Fletouris, D., Theodoridis, A., Zervos, I., Tsantarliotou, M., 2015. Antioxidant effect of crocin on bovine sperm quality and in vitro fertilization. Theriogenology. 84(8):1273-82.
  • Skakkebaek, N.E., Rajpert De Meyts, E., Main, K.M., 2001. Testicular dysgenesis syndrome: an increasingly common developmental disorder with environmental aspects. Hum.Reprod 16: 972−8.
  • Sun,H.,Yang, B., Zhu, C., Liu, R., Wang, H., Li, W., 2013. Presence of metastasis-associated protein 1 in Sertoli cells is required for propercontact between sertoli cells and adjacent germ cells.Urology.81(1):66-73.
  • Vlajkovic, S., Cukuranovic, R., Bjelakovic, M.R., Stefanovic. V. 2012. Possible Therapeutic Use of Spermatogonial Stem Cells in the Treatment of Male Infertility: A Brief Overview. ScientificWorldJournal. 8.
  • Yan Cheng,C.,Dolores, D.M, 2012.The Blood-Testis Barrier and Its Implications for Male Contraception. PharmacolRev. 64(1): 16–64.
  • WHO.,2010. World Health organization laboratory manual for the examination and the processing of human semen. 5 th ed;pp161.

Details

Primary Language English
Subjects Health Care Sciences and Services
Journal Section Clinical Research
Authors

Ayşe ALTUN> (Primary Author)
Department of Obstetrics and Gynaecology School of Medicine,IVF Unıt, Istanbul University
0000-0002-2765-5766
Türkiye


Yasemin ERSOY CANILLIOĞLU>
Department of Histology and Embryology, School of Medicine, Bahçesehir University
0000-0002-3839-6931
Türkiye


Evrim UNSAL>
Genart Woman Health and Reproductive Biotechnology Center
0000-0002-3965-7347
Türkiye


Seda KARABULUT>
Department of Histology and Embryology, Medical Faculty, Istanbul Medipol University
0000-0003-3302-5004
Türkiye


Canan HURDAG>
Department of Histology and Embryology, Medical Faculty, Demiroğlu Bilim University
0000-0003-3336-0830
Türkiye

Publication Date May 1, 2021
Application Date November 20, 2020
Acceptance Date February 7, 2021
Published in Issue Year 2021, Volume 38, Issue 3

Cite

Bibtex @research article { omujecm828811, journal = {Journal of Experimental and Clinical Medicine}, issn = {1309-4483}, eissn = {1309-5129}, address = {}, publisher = {Ondokuz Mayıs University}, year = {2021}, volume = {38}, number = {3}, pages = {272 - 276}, title = {Ultrastructural examination of testicular tissues and evaluation of ZO-1 protein levels obtained from azoospermic cases}, key = {cite}, author = {Altun, Ayşe and Ersoy Canıllıoğlu, Yasemin and Unsal, Evrim and Karabulut, Seda and Hurdag, Canan} }
APA Altun, A. , Ersoy Canıllıoğlu, Y. , Unsal, E. , Karabulut, S. & Hurdag, C. (2021). Ultrastructural examination of testicular tissues and evaluation of ZO-1 protein levels obtained from azoospermic cases . Journal of Experimental and Clinical Medicine , 38 (3) , 272-276 . Retrieved from https://dergipark.org.tr/en/pub/omujecm/issue/62209/828811
MLA Altun, A. , Ersoy Canıllıoğlu, Y. , Unsal, E. , Karabulut, S. , Hurdag, C. "Ultrastructural examination of testicular tissues and evaluation of ZO-1 protein levels obtained from azoospermic cases" . Journal of Experimental and Clinical Medicine 38 (2021 ): 272-276 <https://dergipark.org.tr/en/pub/omujecm/issue/62209/828811>
Chicago Altun, A. , Ersoy Canıllıoğlu, Y. , Unsal, E. , Karabulut, S. , Hurdag, C. "Ultrastructural examination of testicular tissues and evaluation of ZO-1 protein levels obtained from azoospermic cases". Journal of Experimental and Clinical Medicine 38 (2021 ): 272-276
RIS TY - JOUR T1 - Ultrastructural examination of testicular tissues and evaluation of ZO-1 protein levels obtained from azoospermic cases AU - AyşeAltun, YaseminErsoy Canıllıoğlu, EvrimUnsal, SedaKarabulut, CananHurdag Y1 - 2021 PY - 2021 N1 - DO - T2 - Journal of Experimental and Clinical Medicine JF - Journal JO - JOR SP - 272 EP - 276 VL - 38 IS - 3 SN - 1309-4483-1309-5129 M3 - UR - Y2 - 2021 ER -
EndNote %0 Journal of Experimental and Clinical Medicine Ultrastructural examination of testicular tissues and evaluation of ZO-1 protein levels obtained from azoospermic cases %A Ayşe Altun , Yasemin Ersoy Canıllıoğlu , Evrim Unsal , Seda Karabulut , Canan Hurdag %T Ultrastructural examination of testicular tissues and evaluation of ZO-1 protein levels obtained from azoospermic cases %D 2021 %J Journal of Experimental and Clinical Medicine %P 1309-4483-1309-5129 %V 38 %N 3 %R %U
ISNAD Altun, Ayşe , Ersoy Canıllıoğlu, Yasemin , Unsal, Evrim , Karabulut, Seda , Hurdag, Canan . "Ultrastructural examination of testicular tissues and evaluation of ZO-1 protein levels obtained from azoospermic cases". Journal of Experimental and Clinical Medicine 38 / 3 (May 2021): 272-276 .
AMA Altun A. , Ersoy Canıllıoğlu Y. , Unsal E. , Karabulut S. , Hurdag C. Ultrastructural examination of testicular tissues and evaluation of ZO-1 protein levels obtained from azoospermic cases. J. Exp. Clin. Med.. 2021; 38(3): 272-276.
Vancouver Altun A. , Ersoy Canıllıoğlu Y. , Unsal E. , Karabulut S. , Hurdag C. Ultrastructural examination of testicular tissues and evaluation of ZO-1 protein levels obtained from azoospermic cases. Journal of Experimental and Clinical Medicine. 2021; 38(3): 272-276.
IEEE A. Altun , Y. Ersoy Canıllıoğlu , E. Unsal , S. Karabulut and C. Hurdag , "Ultrastructural examination of testicular tissues and evaluation of ZO-1 protein levels obtained from azoospermic cases", Journal of Experimental and Clinical Medicine, vol. 38, no. 3, pp. 272-276, May. 2021