Uzungöl ve Cernek Göllerindeki (Samsun, Türkiye) Tek Hücreli Siyanobakterilerin İzolasyonu ve Karakterizasyonu Üzerine Araştırmalar

Year 2022, Volume: 12 Issue: 2, 167 - 178, 31.12.2022

Kübra Özkul , Haydar Karakaya

https://doi.org/10.54370/ordubtd.1205725

Abstract

Samsun, Bafra Kızılırmak deltasında yer alan Uzungöl ve Cernek göllerindenden alınan su örneklerinden tek hücreli siyanobakterilerin saf kültürler şeklinde izolasyonu gerçekleştirilmiştir. Seyreltilmiş su örnekleri siyanobakteriler için seçici katı besiyeri yüzeyine ekilerek sürekli ışık altında inkübasyona bırakılmış ve izole koloniler elde edilmiştir. Tek hücrelerden oluşmuş 20 koloni tek hücreli izolatlar olarak seçilmiştir. Her bir izolata ait bu tek koloniler sıvı besiyerlerinde sürekli ışık altında sıvı kültürler şekline üretilmiştir. Bu sıvı kültürlerdeki hücrelere ait karakterler incelenerek hangi siyanobakteriyel altbölüme ve hangi form-cinse dahil oldukları belirlenmiştir. Bu çalışmalar sonucunda bütün izolatların Subsection 1 ve Synechococcus form-cinsine dahil olduğu sonucuna varılmıştır. Bu sonucu desteklemek için her bir izolattan genomik DNA izole edilmiş, 16S rRNA gen bölgesi çoğaltılmış ve her bir amplikon EcoRI ile kesilmiştir. Elde edilen fragment büyüklüklerinin standart Synechococcus sp.PCC7942 suşunun fragment büyüklükleriyle uyumlu olduğu belirlenmiştir. Dolayısıyla restriksiyon fragment büyüklükleri ve kültürel özellikler uyumlu olduğundan izolatların tamamının Altbölüm 1 ve Synechococcus form-cinsinin üyeleri olduğu sonucuna varılmıştır.

Keywords

Cernek Gölü, siyanobakteri, synechococcus, uzungöl

Supporting Institution

Ondokuz Mayıs Üniversitesi

Project Number

F373

Thanks

Yazarlar F373 nolu proje ile bu çalışmayı destekleyen Ondokuz Mayıs Üniversitesine teşekkür ederler.

Investigations on Isolation and Characterisation of Unicellular Cyanobacteria in Uzungöl and Cernek Lakes, Samsun, Türkiye

Abstract

Isolation of unicellular cyanobacteria as pure cultures were carried out from the water samples collected from Uzungöl and Cernek lakes in Kızılırmak delta, Bafra, Samsun. Diluted water samples were spread onto a solid BG11 medium which was positively selective for cyanobacteria and isolated single colonies were observed after incubation under continuous light. Twenty colonies composed of unicellular cells were selected as unicellular isolates. These single colonies from each isolates were transferred into a liquid BG11 medium to grow as liquid cultures under continuous light. Characteristics of the cells in liquid cultures of each isolate were used to ıdentify subsection and form-genus of Cyanobacteria they might be included. The results showed that all the isolates were settled in Subsection 1 and form-genus Synechococcus. To support this identification, genomic DNAs were isolated from each of the isolates, 16S rRNA gene regions were amplified, and each amplicon was digested with EcoRI. Restriction fragment lengths were found to be consistent with those of the standard cyanobacterial strain Synechococcus sp. PCC7942. Since restriction fragment lengths and cultural characteristics were consistent, all the isolates were, therefore, evaluated to be included into Subsection 1 and form-genus Synechococcus.

Keywords

cyanobacteria, cernek lake, synechococcus, uzungöl

Project Number

F373

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