Bazı Çürük ve Taze Meyve, Sebze Ekstraktlarının Antioksidan ve Antimikrobiyal Aktiviteleri

Year 2024, Volume: 14 Issue: 1, 9 - 23, 30.06.2024

Aliye Ertürk , Ömer Ertürk

https://doi.org/10.54370/ordubtd.1272380

Abstract

Bu çalışmada bazı taze meyve ve sebzeler ile onların çürük formlarının antimikrobiyal ve antioksidan aktiviteleri değerlendirilmiştir. İncelenen taze ve çürük materyaller arasında Citrus paradise, Citrus sinensis, Punica granatum, Cydonia oblonga, Malus domestica, Citrus limon, Pyrus anatolica var., Persea americana, Capsicum annuum var., Actinidia deliciosa, Beta vulgaris L. yer almaktadır. Taze haldeki meyvelerin ve sebzelerin, potansiyel mikrop öldürücü aktivitelere sahip oldukları zaten biliniyordu. Ancak çürük hallerinin nasıl davranacağı bilinmiyordu. Taze ve çürük numunelerin in vitro antimikrobiyal aktiviteleri seçilmiş bakteri (Bacillus subtilis, Listeria monocytogenes, Staphylococcus aureus, Citrobacter freundii, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa) ve mantar suşları (Candida albicans ve Saccharomyces cerevisiae) üzerinden inhibisyon bölgesine göre ve gelişmiş sayısal araçlarla doğrulanan difüzyon testi ile incelendi. Çürük ve taze nar (24.25±0.09 ve 12.87±0.11 mm) standartlara göre (Ampisilin: 11.76±0.54 ve Cephazolin: 6.00±0.00 mm) S. aeurus'a karşı muazzam aktivite gösterirken; C. albicans'a karşı, çürük avokado (24.12±0.42 mm), Nystatin'e (17.89±0.54 mm) kıyasla tatmin edici bir etki göstermiştir. Antioksidan aktivite ise, DPPH serbest radikal yakalama, demir iyonu şelasyonu, toplam fenolik içerik ve toplam flavonoid içeriği belirleme yöntemleri ile tarandı. 316.21±9.89 mg GAE/g ekstre ile çürük pancar en zengin toplam fenolik madde içeriğine sahipken; çürük greyfurt 118.5±72.58 mg QE/g ekstrakt ile en yüksek toplam flavonoid içeriğini göstermiştir. Sebze ve meyveler üzerine araştırmalar; farmakolojik olarak aktif antimikrobiyal ve antioksidan ajanlar olarak sadece gıda olarak değil, aynı zamanda çürümüş formların da gelecekteki terapötik amaçlar için önerilebileceğini ortaya koymaktadır.

Keywords

antimikrobiyal aktivite, antioksidan aktivite, çürük meyve, çürük sebze

The Antioxidant and Antimicrobial Activities of Some Rotten and Fresh Fruits, Vegetables Extracts

Abstract

This study evaluated the antimicrobial and antioxidant activities of some fresh fruits and vegetables and their rotten forms. Among the fresh and rotten materials examined, there were Citrus paradise, Citrus sinensis, Punica granatum, Cydonia oblonga, Malus domestica, Citrus limon, Pyrus anatolica, Persea americana, Capsicum annuum var., Actinidia deliciosa, Beta vulgaris L. It was already known that fresh fruits, vegetables have potential microbicidal activities. But how the rottens would behave is unknown. Antimicrobial activities of fresh and rotten samples were examined on selected bacterial (Bacillus subtilis, Listeria monocytogenes, Staphylococcus aureus, Citrobacter freundii, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa) and fungal strains (Candida albicans and Saccharomyces cerevisiae) by diffusion test, which was confirmed by the inhibition zone and advanced numerical tools. While rotten and fresh pomegranate (24.25±0.09 and 12.87±0.11 mm) showed tremendous activity against S. aureus by standards (Ampicillin: 11.76±0.54 and Cephazolin: 6.00±0.00 mm); against C. albicans, rotten avocado (24.12±0.42 mm) showed satisfactory potency compared to Nystatin (17.89±0.54 mm). Antioxidant activity was screened by DPPH free radical scavenging, ferrous ion chelation, total phenolic content, and total flavonoid content determination methods. While rotten beetroot has the richest total phenolic content with 316.21 ± 9.89 mg GAE/g extract; rotten grapefruit showed the highest total flavonoid content with 118.57±2.58 mg QE/g extract. Research on vegetables and fruits; reveals that not only as food but also as decay forms can be recommended for future therapeutic purposes as pharmacologically active antimicrobial and antioxidant agents.

Keywords

antimicrobial activity, antioxidant activity, rotten fruit, rotten vegetables

References

• Acuna, U. M., Atha, D. E., Nee, M. H., & Kennelly, E. J. (2002). Antioxidant capacities of ten edible North American plants. Phytotherapy Research, 16(1), 63−65. https://doi.org/10.1002/ptr.1031
• Adamczak, A., O˙zarowski, M., & Karpinski, T. M. (2020). Antibacterial Activity of Some Flavonoids and Organic Acids Widely Distributed in Plants. Journal of Clinical Medicine, 9, 109, 1-17. https://doi.org/10.3390/jcm9010109
• Akbulut, H. F., & Akbulut, M. (2023). Mineral composition, the profile of phenolic compounds, organic acids, sugar and in vitro antioxidant capacity, and antimicrobial activity of organic extracts of Juniperus drupacea fruits. Food Science & Nutrition, 11, 6435–6446. https://doi.org/10.1002/fsn3.3586
• Al-Zoreky, N. S. (2009). Antimicrobial activity of pomegranate (Punica granatum L.) fruit peels. International Journal of Food Microbiology, 134(3), 244–248. https://doi.org/10.1016/j.ijfoodmicro.2009.07.002
• Amao, I. (2018). Health benefits of fruits and vegetables: Review from Sub-Saharan Africa (p. 33-35). IntechOpen. https://dx.doi.org/10.5772/intechopen.74472
• Balowo, A. B., Fayemi, P. O., & Muchenje, V. (2014). Natural antioxidants against lipid–protein oxidative deterioration in meat and meat products: A review. Food Research International, 64, 171–181. https://doi.org/10.1016/j.foodres.2014.06.022
• Bansode, D. S., & Chavan, M. D. (2012). Studies on antimicrobial activity and phytochemical analysis of Citrus fruit juices against selected enteric pathogens. International Research Journal of Pharmacy, 3(11), 122-126. https://irjponline.com/details.php?article=1489
• Bellary, A. N., Sowbhagya, H. B., & Rastogi, N. K. (2011). Osmotic dehydration assisted impregnation of curcuminoids in coconut slices. Journal of Food Engineering, 105(3), 453–459. https://doi.org/10.1016/j.jfoodeng.2011.03.002
• Brand-Williams, W., Cuvelier, M. E., & Berset, C. (1995). Use of a free radical method to evaluate antioxidant activity. LWT-Food Science and Technology, 28(1), 25-30. https://doi.org/10.1016/S0023-6438(95)80008-5
• Cao, G., Ofic, E., & Prior, R. (1997). Antioxidant and Prooxidant Behavior of flavonoids: Structure-activity relationships. Free Radical Biology Medicine, 22(5), 749-760. https://doi.org/10.1016/S0891-5849(96)00351-6
• Cemeroğlu, B. (2013). Meyve Sebze işleme Teknolojisi (5. Baskı, Cilt 1). Nobel Akademik Yayıncılık.
• Chanderbali, A. S., Albert, V. A., Ashworth, V. E. T. M., Clegg, M. T., Litz, R. E., Soltis, D. E., & Soltis, P.S. (2008). Persea americana (avocado): Bringing ancient flowers to fruit in the genomics era. BioEssays, 30(4), 386–396. https://doi.org/10.1002/bies.20721
• Chia, T. W. R., & Dykes, G. A. (2010). Antimicrobial activity of crude epicarp and seed extracts from mature avocado fruit (Persea americana) of three cultivars. Pharmaceutical Biology, 48(7), 753–756. https://doi.org/10.3109/13880200903273922
• Davis, P. H. (1966–1988). Flora of Turkey and the East Aegean Islands (p. 1–10). Edinburgh Univ Press.
• Decker, E. A., & Welch, B. (1990). Role of ferritin as a lipid oxidation catalyst in muscle food. Journal of Agriculture and Food Chemistry, 38, 674-677. https://pubs.acs.org/doi/pdf/10.1021/jf00093a019
• Dhanavade, M. J., Jalkute, C. B., Ghosh, J. S., & Sonawane, K. D. (2011). Study antimicrobial activity of lemon (Citrus lemon L.) peel extract. British Journal of Pharmacology and Toxicology, 2(3), 119-122. https://citeseerx.ist.psu.edu/document?repid=rep1&type=pdf&doi=dc7b0c7076cbf3af14126dbad662869440387e43
• Droby, S. (2006). Improving quality and safety of fresh fruits and vegetables after harvest by the use of biocontrol agents and natural materials. Acta Horticulture, 709, 45-51. https://doi.org/10.17660/ActaHortic.2006.709.5
• Ekwenye, U. N., & Edeha, O. V. (2010). The antibacterial activity of crude leaf extract of Citrus sinensis (sweet orange). International Journal of Pharma and Bio Sciences, 1(4), 743-750. https://citeseerx.ist.psu.edu/document?repid=rep1&type=pdf&doi=ae3ce4527843a5b6c9da62afe9f635e1cade2e7e
• Elkhatim, K. A. S., Elagib, R. A. A., & Hassan, A. B. (2018). Content of phenolic compounds and vitamin C and antioxidant activity in wasted parts of Sudanese citrus fruits. Food Science and Nutrition, 6(5), 1214–1219. https://doi.org/10.1002/fsn3.660
• Ergezer, H., & Serdaroğlu, M. (2009). Et ve et ürünlerinde oksidasyon mekanizması ve antioksidanların kullanımı. Gıda Teknolojisi, 13, 60-64. https://dergipark.org.tr/en/download/article-file/1189253
• Ergezer, H. (2013). Enginar atıklarından elde edilen ekstraktın çiğ ve pişirilmiş köftelerde antioksidatif etkilerinin araştırılması [Doktora tezi]. Ege Üniversitesi.
• Erturk, A. G., Erturk, O., Ayvaz, M. Ç., & Erturk, E. Y. (2018). Screening of phytochemical, antimicrobial and antioxidant activities in extracts of some fruits and vegetables consumed in Turkey. Celal Bayar University Journal of Science, 14(1), 81–92. https://doi.org/10.18466/cbayarfbe.363384
• Fattouch, S., Caboni, P., Coroneo, V., Tuberoso, C. I. G., Angioini, A., Dessi, S., Marzouki, N., & Cabras, P. (2007). Antimicrobial activity of Tunisian quince (Cydonia oblonga Miller) pulp and peel polyphenolic extracts. Journal of Agricultural and Food Chemistry, 55(3), 963-969. https://doi.org/10.1021/jf062614e
• Hussain, H., Mamadalieva, N. Z., Hussain, A., Hassan, U., Rabnawaz, A., Ahmed, I., & Green, I. R. (2022). Fruit Peels: Food Waste as a Valuable Source of Bioactive Natural Products for Drug Discovery. Current Issues Molecular Biology, 44, 1960–1994. https://doi.org/10.3390/cimb44050134
• Javanovic, S. V., Steenken, S., Tosic, M., Marjonovic, B., & Simic, M. G. (1994). Flavonoids as antioksidants. Journal of American Chemical Society, 116, 4846-4851. https://pubs.acs.org/doi/epdf/10.1021/ja00090a032
• Jayas, D. S., & Jeyamkondan, S. (2002). Modified atmosphere storage of grains, meats, fruits and vegetables. Biosystems Engineering, 82(3), 235–251. https://doi.org/10.1006/bioe.2002.0080
• Karadeniz, F., Burdurlu, H. S., Koca, N., & Soyer, Y. (2005). Antioxidant activity of selected fruits and vegetables grown in Turkey. Turkish Journal of Agriculture and Forestry, 29(4), 297-303. https://journals.tubitak.gov.tr/agriculture/vol29/iss4/9
• Lu, P., Bainard, L. D., Ma, B., & Liu, J. (2020). Bio-fertilizer and rotten straw amendments alter the rhizosphere bacterial community and increase oat productivity in a saline–alkaline environment. Scientifc Reports, 10, 19896, 1-11. https://doi.org/10.1038/s41598-020-76978-3
• Mohammed, E. A., Abdalla, I. G., Alfawaz, M. A., Mohammed, M. A., Al Maiman, S. A., Osman, M. A., Yagoub, A. E. A., & Hassan, A. B. (2022). Effects of Extraction Solvents on the Total Phenolic Content, Total Flavonoid Content, and Antioxidant Activity in the Aerial Part of Root Vegetables. Agriculture, 12, 1820, 1-12. https://doi.org/10.3390/agriculture12111820
• Naik, S. N., Goud, V. V., Rout, P. K., & Dalai, A. K. (2010). Production of first and second generation biofuels: A comprehensive review. Renewable and Sustainable Energy Reviews, 14, 578–597. https://doi.org/10.1016/j.rser.2009.10.003
• Nanda, B. L. (2019). Antioxidant and proton potassium ATPase inhibitory activity in fruits. GSC Biological and Pharmaceutical Sciences, 7(3), 107–115. https://doi.org/10.30574/gscbps.2019.7.3.0093
• Nannapaneni, R., Muthaiyan, A., Crandall, P. G., Johnson, M. G., O’Bryan, C. A., Chalova, V. I., Callaway, T. R., Carroll, J. A., Arthington, J. D., Nisbet, D. J., & Ricke, S. C. (2008). Antimicrobial activity of commercial Citrus-based natural extracts against Escherichia coli O157:H7 isolates and mutant strains. Foodborne Pathogenic Disease, 5(5), 695–704. https://doi.org/10.1089/fpd.2008.0124
• Nguyen, V. S., Li, W., Li, Y., & Wang, Q. (2017). Synthesis of citrus polymethoxyflavonoids and their antiproliferative activities on Hela cells. Medicinal Chemistry Research, 26, 1585–1592. https://doi.org/10.1007/s00044-017-1871-4
• Nunes, X. P., Silva, F. S., Almeida, J. R. G. D. S., Barbosa Filho, J. M., de Lima, J. T., de Araújo Ribeiro, L. A., & Júnior, L. J. Q. (2012). Biological Oxidations and Antioxidant Activity of Natural Products. In Global approaches to their role in nutrition and health (Chapter 1, pp. 1-20). INTECH Open Access Publisher. https://doi.org/10.5772/26956
• Park, Y. S., Jung, S. T., Kang, S. G., Heo, B. K. H., Arancibia-Avila, P., Toledo, F., Drzewiecki, J., Namiesnik, J., & Gorinstein, S. (2008). Antioxidants and proteins in ethylenetreated kiwi fruits. Food Chemistry, 107(2), 640-648. https://doi.org/10.1016/j.foodchem.2007.08.070
• Platt, K. A., & Thomson W. W. (1992). Idioblast oil cells of avocado – distribution, isolation, ultrastructure, histochemistry and biochemistry. International Journal of Plant Sciences, 153(3), 301–310. https://doi/abs/10.1086/297033
• Popovic, M., Andjelkovic, U., Grozdanovic, M., Aleksic, I., & GavrovicJankulovic, M. (2013). In vitro antibacterial activity of cysteine protease inhibitor from kiwifruit (Actinidia deliciosa). Indian Journal of Microbiology, 53(1), 100–105. https://doi.org/10.1007/s12088-012-0319-2
• Prasad, K. N., Xie, H., Hao, J., Yang, B., Qiu, S., Wei, X., Chen, F., & Jiang, Y. (2010). Antioxidant and anticancer activities of 8- hydroxypsoralen isolated from wampee [Clausena lansium (Lour.) Skeels] peel. Food Chemistry, 118, 62–66. https://doi.org/10.1016/j.foodchem.2009.04.073
• Ragasa, C. Y., Labrador, P., & Rideout, J. A. (2006). Antimicrobial terpenoids from Lansium domesticum. The Philippine Agriculture Science, 89(1), 101–105. https://animorepository.dlsu.edu.ph/faculty_research/1773/
• Rahmawati, D., Chandra, M., Santoso, S., & Puteri, M. G. (2017). Application of lemon peel essential oil with edible coating agent to prolong shelf life of tofu and strawberry, AIP Conf. Proc, 1803(1). https://doi.org/10.1063/1.4973164
• Rédei, D., Kúsz, N., Rafai, T., Bogdanov, A., Burián, K., Csorba, A., Mándi, A., Kurtán, T., Vasas, A., & Hohmann, J. (2019). 14- Noreudesmanes and a phenylpropane heterodimer from sea buckthorn berry inhibit Herpes simplex type 2 virus replication. Tetrahedron, 75, 1364–1370. https://doi.org/10.1016/j.tet.2019.01.050
• Rodriguez-Aguilera, R., & Oliveira, J. C. (2009). Review of design engineering methods and applications of active and modified atmosphere packaging systems. Food Engineering Reviews, 1, 66–83. https://doi.org/10.1007/s12393-009-9001-9
• Ronald, M. A. (1990). Microbiología. Companía Editorial Continental SA de CV (pp. 505), México DF.
• Samad, A., Azlan, A., & Ismail, A. (2016). Therapeutic effects of vinegar: a review. Current Opinion in Food Science, 8, 56–61. http://doi.org/10.1016/j.cofs.2016.03.001
• Sharma, H., Haq, M. A., Koshariya, A. K., Kumar, A., Rout, S., & Kaliyaperumal, K. (2022). Pseudomonas fluorescens” as an Antagonist to Control Okra Root Rotting Fungi Disease in Plants. Journal of Food Quality, 2022, 5608543, 1-8. https://doi.org/10.1155/2022/5608543
• Shehata, M. M. S. M., & Soltan, S. S. A. (2013). Effects of bioactive component of kiwi fruit and avocado (fruit and seed) on hypercholesterolemic rats. World Journal of Dairy Food Sciences, 8(1), 82-93. https://doi.org/10.5829/idosi.wjdfs.2013.8.1.1121
• Shiban, M. S., Al-Otaibi, M. M., & Al-Zoreky, N. S. (2012). Antioxidant activity of pomegranate (Punica granatum L.) fruit peels. Food Nutrition Sciences, 3(7), 991-996. https://doi.org/10.4236/fns.2012.37131
• Silva, B. M., Andrade, P. B., Martins, R. C., Valentao, P., Ferreres, F., Seabra, R. M., & Ferreira, M. A. (2005). Quince (Cydonia oblonga Miller) fruit characterization using principal component analysis. Journal of Agricultural and Food Chemistry, 53(1), 111−122. https://doi.org/10.1021/jf040321k
• Singh, S., & Immanuel, G. (2014). Extraction of antioxidants from fruit peels and its utilization in paneer. Journal of Food Processing & Technology, 5(7), 349–353. https://doi.org/10.4172/2157-7110.1000349
• Slinkard, K., & Singleton, V. L. (1977). Total phenol analyses: Automation and comparison with manual methods. American Journal of Enology and Viticulture, 28(1), 49-55. https://doi.org/10.5344/ajev.1974.28.1.49
• Sun, H.Y., Ma, N., Pan, T., Du, C.L., & Sun, J.-Y. (2019). Punicagranine, a new pyrrolizine alkaloid with anti-inflammatory activity from the peels of Punica granatum. Tetrahedron Letters, 60, 1231–1233. https://doi.org/10.1016/j.tetlet.2019.03.056
• Umamaheswari, M., & Chatterjee, T. K. (2008). In vitro antioxidant activities of the fractions of Coccinnia grandis L. leaf extract. African Journal of Traditional, Complementary, and Alternative Medicines, 5(1), 61–73. https://doi.org/10.4314/ajtcam.v5i1.31258
• Warda, S., Gadir, A., Mohammed, F., & Bakhiet, A. O. (2007). Antimicrobial activity of Tamarindus indica fruit and Piper nigrum seed. Research Journal of Microbiology, 2(11), 824-830. https://scialert.net/abstract/?doi=jm.2007.824.830
• Zhang, D., Nie, S., Xie, M., & Hu, J. (2020). Antioxidant and antibacterial capabilities of phenolic compounds and organic acids from Camellia oleifera cake. Food Science and Biotechnology, 29, 1, 17–25. https://doi.org/10.1007/s10068-019-00637-1