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Anjiyografi Hasta Plazmalarında, Paraoxonase 1, Arylesterase ve Total Thiol İncelemesi

Year 2022, Volume: 7 Issue: 1, 8 - 12, 01.03.2022
https://doi.org/10.26453/otjhs.905082

Abstract

Amaç: Radyal koroner anjiyografi uygulanan erişkin hastalarda; Paraoxonase-1 (PON 1), Arilesteraz (ARE) ve Total tiyol (TTL) değerlerinin, kan plazmasında tespit edilmesi amaçlandı.
Materyal ve Metot: Çalışmanın katılımcı sayısı, birisi kontrol diğeri hasta grubu olmak üzere, iki grupta toplam 68 oldu. Kontrol grubu 34 sağlıklı bireyden oluşurken, hasta gurubu ise 34 radyal koroner anjiyografi hastasından oluştu. Kan numuneleri lityum heparinli tüplere alındıktan sonra laboratuvara transfer edildi ve burada santrifüj işleminden sonra ayrılan plazmaları, çalışma gününe kadar kapaklı ependorf tüplerde -80C0 de saklandı. Test parametreleri tam otomatik analizörde spektrofotometrik metot ile incelendi. Çalışmada elde edilen veriler SPSS 23 paket programı ile istatistiksel olarak analiz edildi. Anlamlılık düzeyi, p<0,.05 olarak kabul edildi.
Bulgular: Hasta grubundaki ortalama TTL değerleri (360,29 μmol/L) kontrol grubuna göre (482,25 μmol/L) anlamlı derecede düşük bulundu (p<0,001). Benzer şekilde hastaların ortalama ARE değerleri (11,30 U/L) kontrol grubuna göre (14,28 U/L) anlamlı derecede düşük bulundu (p<0,001). Hasta grubu PON 1 (U/L) değerleri sırasıyla (medyan, Q1, Q3): 140,78; 100,06; 322,12 olarak bulundu. Kontrol grubu PON 1 (U/L) değerleri ise, sırasıyla: 153,65; 104,9Q8; 454,13 şeklinde bulundu. Bu değerler arasında anlamlı bir fark olmadığı görüldü (p=0,30).
Sonuç: Anjiyografi hastaları plazma değerleri, lipofilik antioksidan özelliklerde azalma olduğunu gösterdi. PON 1 değerleri, istatistiksel olarak anlamlı olmamasına rağmen bu görüşü destekledi. Öte yandan; hasta grubunda tespit edilen anlamlı TTL düşüklüğü, üzerinde daha geniş araştırmalar gerektiren bir sonuç olarak tespit edildi. 

Supporting Institution

BAP Hızlı Destek

Project Number

12017-08-06-003

References

  • Diepgen TL, Geldmacher MM. Interethnic differences in the detoxification of organophosphates: the human serum paraoxonase polymorphism. Arch Toxicol Suppl. 1986;9:154-158. doi: 10.1007/978-3-642-71248-7_18
  • Bharti M, Mike M, Michael A, Gyorgy P. The Paraoxonases: Their Role in Disease Development and Xenobiotic Metabolism, Proteins and cell regulation. Volume 6. Chapter 15.Page:241-242. Springer Press; 2008.
  • Duni A, Liakopoulos V, Rapsomanikis KP, Dounousi E. Chronic Kidney Disease and Disproportionally Increased Cardiovascular Damage: Does Oxidative Stress Explain the Burden? Oxid Med Cell Longev. 2017;23(3):9036450. doi: 10.1155/2017/9036450
  • Bacchetti T, Ferretti G, Sahebkar A. The role of paraoxonase in cancer. Semin Cancer Biol. Semin Cancer Biol. 2019;56:72-86. doi: 10.1016/j.semcancer.2017.11.013
  • Samouilidou E, Bountou E, Papandroulaki F, Papamanolis M, Papakostas D, Grapsa E. Serum Endocan Levels are Associated With Paraoxonase 1 Concentration in Patients With Chronic Kidney Disease. Ther Apher Dial. 2018;22(4):325-331. doi: 10.1111/1744-9987.12654
  • Pavăl D, Nemeș B, Rusu RL, Dronca E. Genotype-phenotype Analysis of Paraoxonase 1 in Schizophrenic Patients Treated with Atypical Antipsychotics. Clin Psychopharmacol Neurosci. 2018;16(1):32-38. doi: 10.9758/cpn.2018.16.1.32
  • Yeo KK, Armstrong EJ, López JE, et.al. Aspirin and clopidogrel high on-treatment platelet reactivity and genetic predictors in peripheral arterial disease. Catheter Cardiovasc Interv. 2018; 91(7): 1308–1317. doi: 10.1002/ccd.27453
  • Glazko VI, Serov OL, Korochkin LI. Genetic control of the substrate specificity of sheep plasma arylesterase. Article in Russian. Genetika. 1975;11(2):79-86.
  • Cervellati C, Bonaccorsi G, Trentini A, et.al. Paraoxonase, arylesterase and lactonase activities of paraoxonase-1 (PON1) in obese and severely obese women. Scand J Clin Lab Invest. 2018;78(1-2):18-24. doi: 10.1080/00365513.2017.1405274
  • Yazar H, Halis F, Nasir Y, Guzel D, Akdogan M, Gokce A. Effect of the Oxidant-Antioxidant System in Seminal Plasma on Varicocele and Idiopathic Infertility in Male Humans. Clin Lab. 2017;63(5):935-940. doi: 10.7754/Clin.Lab.2016.161111
  • Yuvaci HU, Akdemir N, Bostanci MS, et.al. Evaluation of the level of thiol-disulphide homeostasis in patients with mild and severe preeclampsia. Pregnancy Hypertens. 2016;6(4):394-399. doi: 10.1016/j.preghy.2016.09.003
  • Elmas B, Yildiz T, Yazar H, et.al. New Oxidative Stress Markers Useful in the Diagnosis of Acute Appendicitis in Children: Thiol/Disulfide Homeostasis and the Asymmetric Dimethylarginine Level. Pediatr Emerg Care. 2020;36(8):362-367. doi: 10.1097/PEC.0000000000001339
  • Elmas B, Karacan M, Dervişoğlu P, Kösecik M, İşgüven ŞP, Bal C. Dynamic thiol/disulphide homeostasis as a novel indicator of oxidative stress in obese children and its relationship with inflammatory-cardiovascular markers. Anatol J Cardiol. 2017;18(5):361-369. doi: 10.14744/AnatolJCardiol.2017.7740
  • Connelly PW1, Draganov D, Maguire GF. Paraoxonase-1 does not reduce or modify oxidation of phospholipids by peroxynitrite. Free Radic Biol Med. 2005;38(2):164-74. doi: 10.1016/j.freeradbiomed.2004.10.010
  • Singh K, Singh R, Chandra S, Tyagi S. Paraoxonase-1 is a better indicator than HDL of Atherosclerosis-A pilot study in North Indian population. Diabetes Metab Syndr. 2018;12(3):275-278. doi: 10.1016/j.dsx.2017.12.006
  • Moya C, Máñez S. Paraoxonases: metabolic role and pharmacological projection. Naunyn Schmiedebergs Arch Pharmacol. 2018;391(4):349-359. doi: 10.1007/s00210-018-1473-9
  • Borovkova EI, Antipova NV, Komeenko TV, Shakhparonov MI, Borovkov IM. Paraoxonase: The Universal Factor of Antioxidant Defense in Human Body. Vestn Ross Akad Med Nauk. 2017;72(1):5-10. doi: 10.15690/vramn764
  • Ding J, Chen Q, Zhuang X, Feng Z, Xu L, Chen F. Low paraoxonase 1 arylesterase activity and high von Willebrand factor levels are associated with severe coronary atherosclerosis in patients with non-diabetic stable coronary artery disease. Med Sci Monit. 2014;20:2421-2429. doi: 10.12659/msm.890911
  • Kundi H, Erel Ö, Balun A, et.al. Association of thiol/disulfide ratio with syntax score in patients with NSTEMI. Scand Cardiovasc J. 2015;49(2):95-100. doi: 10.3109/14017431.2015.1013153

Paraoxonase1, Arylesterase and Total Thiol Investigation in Angiography Patient Plasmas

Year 2022, Volume: 7 Issue: 1, 8 - 12, 01.03.2022
https://doi.org/10.26453/otjhs.905082

Abstract

Objective: In adult patients undergoing radial coronary angiography; It was aimed to determine the values ​​of paraoxonase-1, Arylesterase and Total thiol in blood plasma.
Materials and Methods: The number of participants in the study was 68 in two groups, one for the control group and the other for the patient group. While the control group consisted of 34 healthy individuals, the patient group consisted of 34 radial coronary angiography patients. After blood samples were taken into lithium heparin tubes, they were transferred to the laboratory and the plasma separated after centrifugation was stored at -80 °C in capped eppendorf tubes until the working day. Test parameters were analyzed by spectrophotometry method on a fully automated analyzer. The data obtained in the study were statistically analyzed with the SPSS 23 package program. Significance level was accepted as p<0.05.
Results: The mean TTL values ​​in the patient group (360.29 μmol/L) were found to be significantly lower than the control group (482.25 μmol/L) (p <0.001). Similarly, the mean ARE values ​​of the patients (11.30 U/L) were found to be significantly lower than the control group (14.28 U/L) (p<0.001). Patient group PON 1 (U/L) values, respectively (median, Q1, Q3): 140.78; 100.06; 322.12 were found. Control group PON 1 (U/L) values: 153.65; 104.9Q8; 454.13 were found. There was no significant difference between these values (p=0.30).
Conclusion: Angiography patients showed a decrease in plasma values, lipophilic antioxidant properties. PON 1 values supported this view, although it was not statistically significant. On the other hand; Significant low TTL detected in the patient group was determined as a result that requires further studies. 

Project Number

12017-08-06-003

References

  • Diepgen TL, Geldmacher MM. Interethnic differences in the detoxification of organophosphates: the human serum paraoxonase polymorphism. Arch Toxicol Suppl. 1986;9:154-158. doi: 10.1007/978-3-642-71248-7_18
  • Bharti M, Mike M, Michael A, Gyorgy P. The Paraoxonases: Their Role in Disease Development and Xenobiotic Metabolism, Proteins and cell regulation. Volume 6. Chapter 15.Page:241-242. Springer Press; 2008.
  • Duni A, Liakopoulos V, Rapsomanikis KP, Dounousi E. Chronic Kidney Disease and Disproportionally Increased Cardiovascular Damage: Does Oxidative Stress Explain the Burden? Oxid Med Cell Longev. 2017;23(3):9036450. doi: 10.1155/2017/9036450
  • Bacchetti T, Ferretti G, Sahebkar A. The role of paraoxonase in cancer. Semin Cancer Biol. Semin Cancer Biol. 2019;56:72-86. doi: 10.1016/j.semcancer.2017.11.013
  • Samouilidou E, Bountou E, Papandroulaki F, Papamanolis M, Papakostas D, Grapsa E. Serum Endocan Levels are Associated With Paraoxonase 1 Concentration in Patients With Chronic Kidney Disease. Ther Apher Dial. 2018;22(4):325-331. doi: 10.1111/1744-9987.12654
  • Pavăl D, Nemeș B, Rusu RL, Dronca E. Genotype-phenotype Analysis of Paraoxonase 1 in Schizophrenic Patients Treated with Atypical Antipsychotics. Clin Psychopharmacol Neurosci. 2018;16(1):32-38. doi: 10.9758/cpn.2018.16.1.32
  • Yeo KK, Armstrong EJ, López JE, et.al. Aspirin and clopidogrel high on-treatment platelet reactivity and genetic predictors in peripheral arterial disease. Catheter Cardiovasc Interv. 2018; 91(7): 1308–1317. doi: 10.1002/ccd.27453
  • Glazko VI, Serov OL, Korochkin LI. Genetic control of the substrate specificity of sheep plasma arylesterase. Article in Russian. Genetika. 1975;11(2):79-86.
  • Cervellati C, Bonaccorsi G, Trentini A, et.al. Paraoxonase, arylesterase and lactonase activities of paraoxonase-1 (PON1) in obese and severely obese women. Scand J Clin Lab Invest. 2018;78(1-2):18-24. doi: 10.1080/00365513.2017.1405274
  • Yazar H, Halis F, Nasir Y, Guzel D, Akdogan M, Gokce A. Effect of the Oxidant-Antioxidant System in Seminal Plasma on Varicocele and Idiopathic Infertility in Male Humans. Clin Lab. 2017;63(5):935-940. doi: 10.7754/Clin.Lab.2016.161111
  • Yuvaci HU, Akdemir N, Bostanci MS, et.al. Evaluation of the level of thiol-disulphide homeostasis in patients with mild and severe preeclampsia. Pregnancy Hypertens. 2016;6(4):394-399. doi: 10.1016/j.preghy.2016.09.003
  • Elmas B, Yildiz T, Yazar H, et.al. New Oxidative Stress Markers Useful in the Diagnosis of Acute Appendicitis in Children: Thiol/Disulfide Homeostasis and the Asymmetric Dimethylarginine Level. Pediatr Emerg Care. 2020;36(8):362-367. doi: 10.1097/PEC.0000000000001339
  • Elmas B, Karacan M, Dervişoğlu P, Kösecik M, İşgüven ŞP, Bal C. Dynamic thiol/disulphide homeostasis as a novel indicator of oxidative stress in obese children and its relationship with inflammatory-cardiovascular markers. Anatol J Cardiol. 2017;18(5):361-369. doi: 10.14744/AnatolJCardiol.2017.7740
  • Connelly PW1, Draganov D, Maguire GF. Paraoxonase-1 does not reduce or modify oxidation of phospholipids by peroxynitrite. Free Radic Biol Med. 2005;38(2):164-74. doi: 10.1016/j.freeradbiomed.2004.10.010
  • Singh K, Singh R, Chandra S, Tyagi S. Paraoxonase-1 is a better indicator than HDL of Atherosclerosis-A pilot study in North Indian population. Diabetes Metab Syndr. 2018;12(3):275-278. doi: 10.1016/j.dsx.2017.12.006
  • Moya C, Máñez S. Paraoxonases: metabolic role and pharmacological projection. Naunyn Schmiedebergs Arch Pharmacol. 2018;391(4):349-359. doi: 10.1007/s00210-018-1473-9
  • Borovkova EI, Antipova NV, Komeenko TV, Shakhparonov MI, Borovkov IM. Paraoxonase: The Universal Factor of Antioxidant Defense in Human Body. Vestn Ross Akad Med Nauk. 2017;72(1):5-10. doi: 10.15690/vramn764
  • Ding J, Chen Q, Zhuang X, Feng Z, Xu L, Chen F. Low paraoxonase 1 arylesterase activity and high von Willebrand factor levels are associated with severe coronary atherosclerosis in patients with non-diabetic stable coronary artery disease. Med Sci Monit. 2014;20:2421-2429. doi: 10.12659/msm.890911
  • Kundi H, Erel Ö, Balun A, et.al. Association of thiol/disulfide ratio with syntax score in patients with NSTEMI. Scand Cardiovasc J. 2015;49(2):95-100. doi: 10.3109/14017431.2015.1013153
There are 19 citations in total.

Details

Primary Language Turkish
Subjects Health Care Administration
Journal Section Research article
Authors

Hayrullah Yazar 0000-0001-9447-6322

Güler Kuşçu Günay 0000-0002-5529-3061

Mustafa Baran İnci 0000-0003-1893-5368

Süleyman Kaleli 0000-0002-6043-2521

Mehmet Çakar 0000-0003-3216-4205

Project Number 12017-08-06-003
Publication Date March 1, 2022
Submission Date March 29, 2021
Acceptance Date January 13, 2022
Published in Issue Year 2022 Volume: 7 Issue: 1

Cite

AMA Yazar H, Kuşçu Günay G, İnci MB, Kaleli S, Çakar M. Anjiyografi Hasta Plazmalarında, Paraoxonase 1, Arylesterase ve Total Thiol İncelemesi. OTJHS. March 2022;7(1):8-12. doi:10.26453/otjhs.905082

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