pam med j Pamukkale Medical Journal 1308-0865 Pamukkale University 10.31362/patd.1756853 Urology Üroloji Biochemical and histomorphometric comparison of antioxidant (Taurine) and ozone therapy effects on ESWL-induced oxidative damage in rabbit kidney tissue Tavşan böbrek dokusunda eswl ile indüklenen oksidatif hasar üzerine antioksidan (Taurin) ve ozon terapi etkilerinin biyokimyasal ve histomorfometrik karşılaştırılması https://orcid.org/0000-0003-1542-0393 Görgü Nart BANDIRMA ONYEDİ EYLÜL ÜNİVERSİTESİ, TIP FAKÜLTESİ https://orcid.org/0000-0003-0457-512X Başer Aykut BANDIRMA ONYEDİ EYLÜL ÜNİVERSİTESİ, TIP FAKÜLTESİ https://orcid.org/0000-0002-4732-1923 Başar Mehmet Murad 04 13 2026 19 2 373 382 08 02 2025 09 29 2025 Copyright © 2008, Pamukkale Medical Journal 2008 Pamukkale Medical Journal

Purpose: This study aimed to investigate the role of free radicals in kidney injury induced by extracorporeal shock wave lithotripsy (ESWL) and to compare the protective effects of taurine and ozone therapy.Methods: Thirty male New Zealand rabbits were divided into five groups: control, ESWL, ESWL + ozone, ESWL + taurine, and ESWL + ozone + taurine. ESWL was applied in a single session at 14 kV and 2500 shocks. Ozone was administered intraperitoneally and taurine orally for 15 days. Histopathological evaluations and biochemical analyses, including MDA, SOD, CAT, and GSH-Px levels, were performed on renal tissues.Results: ESWL significantly increased tissue MDA levels and decreased antioxidant enzyme levels. Taurine and ozone treatments reduced oxidative stress by lowering MDA levels and enhancing antioxidant enzyme activity. Histologically, less tissue damage was observed in the treatment groups.Conclusion: Taurine and ozone therapies effectively reduced ESWL-induced kidney damage both biochemically and histologically. These findings support the potential protective role of these agents in clinical settings.

Amaç: Bu çalışmada, litotripsi sonrası oluşan böbrek hasarında serbest radikallerin rolü ve bu hasarın azaltılmasında taurin ve ozon tedavisinin etkileri karşılaştırılmıştır.Gereç ve yöntem: Otuz erkek Yeni Zelanda tavşanı beş gruba ayrıldı: kontrol, ESWL, ESWL + ozon, ESWL + taurin ve ESWL + ozon + taurin. ESWL uygulaması tek seans 14 kV enerjiyle, 2500 atım olarak gerçekleştirildi. Ozon tedavisi intraperitoneal olarak, taurin ise oral yolla 15 gün süreyle verildi. Histopatolojik değerlendirme yanı sıra doku düzeyinde MDA, SOD, CAT, GSH-Px gibi biyokimyasal parametreler ölçüldü.Bulgular: ESWL sonrası doku MDA düzeyleri anlamlı şekilde artarken, antioksidan enzim düzeyleri azaldı. Taurin ve ozon tedavileri, MDA düzeylerini düşürüp antioksidan enzim düzeylerini artırarak oksidatif stresi azalttı. Histopatolojik olarak da tedavi gruplarında daha az hasar izlendi.Sonuç: Taurin ve ozon tedavisi, ESWL'nin neden olduğu böbrek hasarını hem biyokimyasal hem de histopatolojik olarak azaltmada etkili bulunmuştur. Bu sonuçlar, klinik uygulamalarda bu ajanların koruyucu potansiyelini desteklemektedir.

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