Comparison of MR imaging and orcein-picroindigocarmine staining method in fetal sectional anatomy

Tuncay Peker; Nadir Gülekon; Nil Tokgöz; Suna Ömeroğlu; Afitap Anıl; Hasan Basri Turgut

Fetüs kesitsel anatomisinde MR ve orcein-picroindigocarmine boyama yönteminin karşılaştırılması

Year 2014, Issue: 1, 33 - 40, 01.04.2014

Tuncay Peker Nadir Gülekon Nil Tokgöz Suna Ömeroğlu Afitap Anıl Hasan Basri Turgut

Abstract

AMAÇ: Literatürde fetüs kesitsel anatomisi ile ilgili çalışmalar kısıtlıdır. Bu çalışmada fetüs kesitsel anatomik yapılarını incelenmek amacıyla orcein-picroindigocarmine boyama yöntemi ile fetüs manyetik rezonans görüntüleme kesitleri karşılaştırılmıştır. YÖNTEMLER: 18-20 haftalık 6 insan fetüsunun baş-boyun ve ekstremiteleri önce manyetik rezonans ile görüntülendikten sonra kesitler alınarak orcein-picroindigocarmine ile boyandı. BULGULAR: Baş kesitlerinde orcein-picroindigocarmine boyama ile saptanabilen küçük damarlar MR görüntülerinde izlenememiştir. Ayrıca histolojik kesitlerde 20 haftalık bir fetüste oksipital lobda girus oluşumu saptanırken, aynı fetüsün MR görüntülerinde böyle bir bulguya rastlanmamıştır. Boyun bölgesinde histolojik kesitler ile MR görüntüleri arasında ince detaylar dışında belirgin farklılık saptanmamışken, özellikle ekstremitelerde orcein-picroindigocarmine boyama yumuşak doku planlarının ayırt edilmesinde, kas ve damar-sinir paketinin gösterilmesinde manyetik rezonans görüntülemeye üstün bulunmuştur. SONUÇ: Bu konuda daha detaylı çalışmaların yapılması gerektiği inancındayız.

Keywords

Fetus, MR, histokimyasal boyama, kesitsel anatomi

Comparison of MR imaging and orcein-picroindigocarmine staining method in fetal sectional anatomy

Year 2014, Issue: 1, 33 - 40, 01.04.2014

Tuncay Peker Nadir Gülekon Nil Tokgöz Suna Ömeroğlu Afitap Anıl Hasan Basri Turgut

Abstract

Purpose: There are limited fetal MR anatomy studies in the literature. The aim of this study was to compare
the orcein-picroindigocarmine staining method with fetal MR imaging in evaluating sectional fetal anatomic
structures.
Materials and methods: After MR images of 6 human 18-20 week old fetal sections of the head, neck and
extremities were taken, the same sections were stained with orcein-picroindigocarmine stain.
Results: The orcein-picroindigocarmine staining method had some advantages over MR imaging in regards to
neurovascular structures and gyrus formation. Gyrus formation in the occipital lobe of a 20 week old fetus was
detected in the histological specimens whereas it was not detected in the MR images of the same fetus. In the
neck region, no evident difference, except for slight details, were identified between orcein-picroindigocarmine
staining and MR imaging. Particularly in the extremities, Orcein-picroindigocarmine staining was found to be
superior to MR imaging in differentiating between soft tissue planes, muscle, and neuro-vascular bundles.
Conclusion: The results from this study warrant further investigation.

Keywords

Fetus, MR, histochemical staining, sectional anatomy

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Details

Other ID	JA48ZN48YM
Journal Section	Research Article
Authors	Tuncay Peker This is me Nadir Gülekon This is me Nil Tokgöz This is me Suna Ömeroğlu This is me Afitap Anıl This is me Hasan Basri Turgut This is me
Publication Date	April 1, 2014
Submission Date	April 1, 2014
Published in Issue	Year 2014 Issue: 1

Cite

APA	Peker, T., Gülekon, N., Tokgöz, N., Ömeroğlu, S., et al. (2014). Comparison of MR imaging and orcein-picroindigocarmine staining method in fetal sectional anatomy. Pamukkale Medical Journal(1), 33-40.
AMA	Peker T, Gülekon N, Tokgöz N, Ömeroğlu S, Anıl A, Turgut HB. Comparison of MR imaging and orcein-picroindigocarmine staining method in fetal sectional anatomy. Pam Med J. April 2014;(1):33-40.
Chicago	Peker, Tuncay, Nadir Gülekon, Nil Tokgöz, Suna Ömeroğlu, Afitap Anıl, and Hasan Basri Turgut. “Comparison of MR Imaging and Orcein-Picroindigocarmine Staining Method in Fetal Sectional Anatomy”. Pamukkale Medical Journal, no. 1 (April 2014): 33-40.
EndNote	Peker T, Gülekon N, Tokgöz N, Ömeroğlu S, Anıl A, Turgut HB (April 1, 2014) Comparison of MR imaging and orcein-picroindigocarmine staining method in fetal sectional anatomy. Pamukkale Medical Journal 1 33–40.
IEEE	T. Peker, N. Gülekon, N. Tokgöz, S. Ömeroğlu, A. Anıl, and H. B. Turgut, “Comparison of MR imaging and orcein-picroindigocarmine staining method in fetal sectional anatomy”, Pam Med J, no. 1, pp. 33–40, April 2014.
ISNAD	Peker, Tuncay et al. “Comparison of MR Imaging and Orcein-Picroindigocarmine Staining Method in Fetal Sectional Anatomy”. Pamukkale Medical Journal 1 (April 2014), 33-40.
JAMA	Peker T, Gülekon N, Tokgöz N, Ömeroğlu S, Anıl A, Turgut HB. Comparison of MR imaging and orcein-picroindigocarmine staining method in fetal sectional anatomy. Pam Med J. 2014;:33–40.
MLA	Peker, Tuncay et al. “Comparison of MR Imaging and Orcein-Picroindigocarmine Staining Method in Fetal Sectional Anatomy”. Pamukkale Medical Journal, no. 1, 2014, pp. 33-40.
Vancouver	Peker T, Gülekon N, Tokgöz N, Ömeroğlu S, Anıl A, Turgut HB. Comparison of MR imaging and orcein-picroindigocarmine staining method in fetal sectional anatomy. Pam Med J. 2014(1):33-40.