Kaempferol, kurkumin ve kaempferol-kurkumin kombinasyonunun deneysel akut pankreatitteki etkileri

Year 2020, Volume: 13 Issue: 1, 101 - 108, 21.01.2020

Musa Turgut Halil Kocamaz , Esin Avcı , Mutlu Yaka Gülçin Abban Mete

https://doi.org/10.31362/patd.644131

Abstract

Amaç:
Akut Pankreatit ani başlayan karın ağrısı ile kendini
gösterip sistemik inflamatuar yanıta kadar ilerleyebilen acil bir klinik
durumdur. Standart bir tedavisi olmayan bu hastalıkta güncel tedavi
yaklaşımlarına gereksinim vardır. Deneysel akut pankreatitte kaempferol,
kurkumin ve kaempferol-kurkumin kombinasyonunun etkilerini göstermeyi amaçladık.

Gereç
ve Yöntem: Wistar albino erişkin
ratlar altı gruba ayrıldı. Grup I: Sıçanlara fizyolojik su intraperitoneal (i.p.) olarak
verildi. Grup II: L-arjinin i.p. olarak 250 mg/100 gr, Grup III(DMSO): İ.p.
olarak 1 ml/100 gr Dimetil sülfoksit (DMSO), Grup IV(L-Arjinin + Kurkumin): i.p. 
L-Arginin ve kurkumin 50 mg/kg İP, Grup V(L-Arjinin + Kaempferol): İ.p.  
L-Arginin ve kaempferol
21 mg/kg İP, Grup VI(L-Arjinin + Kurkumin + Kaempferol): İ.p.  L-Arginin , kurkumin 50 mg/kg ve kaempferol 21 mg/kg i.p. uygulandı. Deney
bitiminde kan ve pankreas örnekleri alındı. Histolojik, biyokimyasal ve
immunohistokimyasal incelemeler yapıldı.

Bulgular:
Malondialdehid,
TNF-α ve IL-6 düzeyleri gruplar arasında farklı değildi. Superoksit
dismutaz(SOD)  düzeyleri grup VI’da diğer
gruplara göre yüksekti ancak istatistiksel olarak anlamlı değildi. Ödem skoru
grup VI’da grup II’ye göre daha düşük idi. Grup V ve grup VI’da inflamasyon
skoru grup II’ den daha az idi. Nekroz skoru grup IV, grup V ve grup VI’da grup
II’den daha az idi. Grup II’deki NF(nükleer faktör)-κB boyanma skoru tedavi
gruplarından farklı değildi.

Sonuç:
L-arjinin ile
oluşturulan deneysel akut pankreatitde kaempferolün ve kaempferol-kurkumin
kombinasyonunun özellikle histopatolojik olarak inflamasyonu ve nekrozu
azalttığı bu sayede koruyucu etkileri olduğu görülmüştür.

Keywords

arjinin, kurkumin, inflamasyon

Supporting Institution

Pamukkale Üniversitesi

Project Number

2018TIPF02

Thanks

Bu çalışma, Pamukkale Üniversitesi Bilimsel Araştırma Projeleri Birimi tarafından desteklenmiştir. Proje No: 2018TIPF026

The ameliorative effects of kaempferol, curcumin and kaempferol-curcumin combination on experimental acute pancreatitis

Abstract

Aim: Acute pancreatitis is a medical emergency disorder which
manifests itself with sudden onset abdominal pain and may progress systemic
inflammatory response syndrome. Novel treatment modalities are required for
this disorder which does not have standard treatment regime. We aimed to evaluate
the effects of kaempferol, curcumin and kaempferol-curcumin combination on
experimental acute pancreatitis.

Material
and method: Wistar albino adult rats were divided into 6
groups. Group I: saline was given by intraperitoneal(ip). Group II: L-arginine was given by
ip at a dose of 250 mg per 100 gr. Group III(DMSO):
Dimethyl sulphoxide (DMSO) was given by ip at a dose of 1 ml/100 gr. Group IV(L-arginine+curcumin):
L-arginine and curcumin at a
dose of 50 mg/kg was given by ip, Group V(L-arginine+
Kaempferol): L-arginine and
kaempferol at a dose of 21
mg/kg was
given by ip. Group VI(L-arginine+curcumin + Kaempferol): L-arginine, curcumin at a dose of 50
mg/kg and kaempferol at a
dose of  21 mg/kg was given by ip. Blood and
pancreas sample of the rats were obtained.

Results:
Malondialdehyde
and IL-6 levels were not different between groups. Superoxide dismutase(SOD)
levels were higher than other groups but it was not statistically significant.
Edema score on pancreas tissue were lower than group II in group VI.
Inflammatıon score were lower than group II in groups V and VI. NF(nuclear
factor)- κB staining score were not different between groups.

Conclusion:
Kaempferol and
kaempferol-curcumin combination have protective effects on L-arginine induced
experimental acute pancreatitis by diminishing inflammation and necrosis.

Keywords

arginine, curcumin, inflammation

Project Number

2018TIPF02

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