Hydroxyapatite Coating on Ti6Al4V Alloy Surface Through Biomimetic Method Using Glycolic Acid - Sodium Gluconate Buffer System and Examination of Properties of the Coating

Year 2017, Volume: 20 Issue: 4, 993 - 1001, 20.12.2017

İbrahim Aydın Ferdi Engin

https://doi.org/10.2339/politeknik.369141

Cited By: 3

Abstract

The use of implant materials is one of the important
surgical interventions in the treatment of disorders that are congenital and acquired
deformities in musculoskeletal system. Metallic biomaterials within these
implant materials are often preferred because of their proximity to the
mechanical properties of the bone. In the implantation process, the surfaces of
these alloys are coated with ceramic based biomaterials to increase the
bioactivity and biocompatibility properties.

In this study, it is aimed to create hydroxyapatite
(HA) coating that is completely harmonious with human blood plasma environment,
on Ti6Al4V alloy, using glycolic acid - sodium gluconate buffer system with
biomimetic method for the first time in literature. Coating was realised inside
synthetic body fluid (SBF) with waiting periods of 24, 48, 72 and 96 hours.
Relating to the coatings, surface smoothness and thickness specifications have
been determined, their micro structure has been analysed by Scanning Electron
Microscope (SEM), the elementary analyses namely Energy Dispersive X-ray
Spectroscopy (EDS) for the surfaces of coating have been determined and X-Ray
Diffraction (XRD) analysis have been conducted for obtaining the concentrations
of the phases. As a result of the experiments, successful surface smoothness
values and ratio of Ca/P close to optimal value have been obtained.
Additionally, the presence of HA crystals with intense phase structure, with
compact and homogeneous distribution on the substrate have been observed.

Keywords

Biomimetic coating, glycolic acid - sodium gluconate, Hydroxyapatite (HA), Ti6Al4V, Synthetic Body Fluid (SBF)

References

• 1. Özkurt B. and Tabak A. Y., “Metallic biomaterials and metallozis”, Journal of TOTBİD, 10(2): 83-86, (2011). 2. Pasinli A. and Aksoy R. S., “Hydroxyapatite for artificial bone applications”, Biotechnology Magazine, 1: 41-51, (2010). 3. Aydın İ., “An investigation of fracture and wear behavior of HA coatings deposited onto Ti6Al4V alloys in a new environment”, PhD Thesis, Celal Bayar University, Institute of Science, (2013). 4. Aydın İ., Çetinel H., Pasinli A. and Yüksel M., “Fracturing and adhesion behavior of hydroxyapatite formed by a citric acid and sodium citrate buffer system”, Materials Testing, 58(2): 1 - 6, (2016). 5. Pasinli A., “Hydroxyapatite for artificial bone applications”, Electronic Journal of Machine Technologies, 25 - 35, (2004). 6. Kokubo T., Kim H. M., Miyaji F., Takadama H. and Miyazaki T., “Ceramic-metal and ceramic-polymer composites prepared by a biomimetic process”, Composites Part A, Applied Science and Manufacturing, 30: 405–409, (1999). 7. Taş A. C., “Synthesis of biomimetic Ca-hydroxyapatite powders at 37 ºC in synthetic body fluids”, Biomaterials, 21: pp. 1429–1438, (2000) 8. Sepahvandi A., Moztarzadeh F., Mozafari M., Ghaffari M., and Raee N., “Photoluminescence in the characterization and early detection of biomimetic bone-like apatite formation on the surface of alkaline-treated titanium implant”, State of the art. Biointerfaces, 86: 390-396, (2011). 9. Faure J., Balamurugan A., Benhayoune H., Torres P., Balossier G. and Ferreira J. M. F., “Morphological and chemical characterization of biomimetic bone like apatite formation on Ti6Al4V titanium alloy”, Materials Science and Engineering, 29: 1252-1257, (2009). 10. Li F., Feng Q. L., Cui F. Z., Li H. D. and Schubert H., “A simple biomimetic method for calcium phosphate coating”, Surface & Coating Technology, 154: 88–93, (2002). 11. Xiaobo C., Yuncang L., Peter D. H. and Cui’e W., “Microstructures and bond strengths of the calcium phosphate coatings formed on titanium from different simulated body fluids”, Materials Science and Engineering, 29: 165-171, (2009). 12. Pasinli A., Yüksel M., Çelik E., Şener S. and Taş C. A., “A new approach in biomimetic synthesis of calcium phosphate coatings using lactic acid-Na lactate buffered body fluid solution”, Acta Biomaterialia, 6: 2282-2288, (2010). 13. Çağlayan M. E., “Hydroxyapatite coating of Ti6A14V alloys in alanin - alanine sodium salt environment with biomimetic method and observing of some features”, M.Sc. Thesis, Celal Bayar University, Institute of Science, (2016). 14. Kırman M., “Coating Ti6Al4V alloy by hydroxyapatite through biomimetic method using aminoacetic acid - sodium aminoacetate buffer system and examination of features of the coating”, M.Sc. Thesis, Celal Bayar University, Institute of Science, (2016). 15. Hayakawa T., Yoshinari M., Kiba H., Yamamoto H., Nemoto K., Jansen J. A., “Trabecular bone response to surface roughened and calcium phosphate (Ca-P) coated titanium implants”, Biomaterials, 23: 1025–1031, (2002). 16. Yoshinari M., Oda Y., Inoue T., Matsuzaka K., Shimono M., “Bone response to calcium phosphate coated and bisphosphonate immobilized titanium implants”, Biomaterials, 23: 2879 – 2885, (2002). 17. Citeau A., Guicheux J., Vinatier C., Layrolle P., Nguyen T. P., Pilet P. and Daculsi G., “In vitro biological effects of titanium rough surface obtained by calcium phosphate grid blasting”, Biomaterials, 26: 157 – 165, (2005). 18. Aydın İ., Çetinel H., Pasinli A., and Yüksel M., “Preparation of hydroxyapatite coating by using citric acid sodium citrate buffer system in the biomimetic procedure”, Materials Testing, 58(2): 140 – 145, (2013). 19. Aydın İ., Çaglayan M. E. and Pasinli A., “Hydroxyapatite coating of Ti6Al4V alloys in alanin - alanine sodium salt environment with biomimetic method”, Celal Bayar University Journal of Science, 12(2): 201 – 207, (2016). 20. Aydın İ., Kırman M. and Pasinli A., “Coating Ti6Al4V alloy by hydroxyapatite through biomimetic method using aminoacetic acid - sodium aminoacetate buffer system and examination of features of the coating“, 1st International Mediterranean Science and Engineering Congress, 26 - 28 October, Adana, Turkey, (2016). 21. Şimşek F. A., “Chemical preparation of calcium hydroxyapatite in synthetic body fluids at 37 °C and its use for coating some metal surfaces”, M.Sc. Thesis, Middle East Technical University, Institute of Science, 1-107, (1997). 22. Nagano M., Kitsugi T., Nakamura T., Kokuba T. and Tanahashi M., “Bone bonding ability of an apatite coated polymer produced using a biomimetic method: A mechanical and histological study in vivo”, Journal of Biomedical Materials Research Part A, 32: 487-494, (1996). 23. Urist M. R., Lietze A. and Dawson E., “β tricalcium delivery system for bone morphogenetic protein”, Clinical Orthopedics and Related Research, 187: 227-280, (1984). 24. Despina D. Deligianni, Nikoleta D. Katsala, Petros G. Koutsoukos and Yiannis F. Missirlis., “Effect of surface roughness of hydroxyapatite on human bone marrow cell adhesion, proliferation, dilerentiation and detachment strength”, Biomaterials, 22: 87 – 96, (2001). 25. Han J. K., Song H.Y., Saito F. and Lee B. T., “Synthesis of high purity nano-sized hydroxyapatite powder by microwave-hydrothermal method”, Materials Chemistry and Physics, 99: 235 – 239, (2006). 26. Takadama H., Kim H. M., Kukuba T. and Nakamura T., “TEM-EDX study of mechanism of bonelike apatite formation on bioactivite titanium metal in simulated body fluid”, Journal of Biomedical Materials Research, 57: 441 – 448, (2001). 27. Barrere F., Van Blitterswijk C. A., Groot K. and Layrolle P., “Influence of ionic strength and carbonate on the Ca-P coating formation from SBFx5 solution”, Biomaterials, 23: 1921 – 1930, (2002).