Response Surface Methodology for Optimization of Antagonist Effect of Bacillus sp.

Year 2024, Volume: 5 Issue: 1, 126 - 132, 30.06.2024

Ecren Uzun Yaylacı

https://doi.org/10.53501/rteufemud.1448254

Abstract

The study aimed to optimize bacterial culture conditions to enhance the antagonist effect of B. pumilus PJ_11 on V. vulnificus. The experiment designed by the Box Behnken method showed that the antagonistic effect was highest (26.44 mm) at 30°C, NaCl concentration 2%, and 18 hours. According to the coefficient values of the regression equation, temperature was more effective on the response than other parameters. The combined effect of two variables, temperature and NaCl concentration, was more effective than other combinations. The results showed that the Box-Behnken design was an adequate method to optimize three factors of culture conditions.

Keywords

Bacillus sp., Response Surface Methodology, Antagonistic effect

Bacillus sp.'nin Antagonist Etkisinin Optimize Edilmesine Yönelik Tepki Yüzey Metodolojisi

Abstract

Bu çalışmada, B. pumilus PJ_11'in V. vulnificus üzerindeki antagonist etkisini arttırmak için bakteri kültürü koşullarını optimize etmek amaçladı. Box Behnken yöntemiyle tasarlanan deney, antagonist etkinin 30°C'de, %2 NaCl konsantrasyonunda ve 18. saatte en yüksek (26,44 mm) olduğunu gösterdi. Regresyon denkleminin katsayı değerlerine göre sıcaklığın yanıt üzerinde diğer parametrelere göre daha etkili olduğu belirlendi. İki değişkenin (sıcaklık ve NaCl konsantrasyonu) birleşik etkisinin diğer kombinasyonlardan daha etkili olduğu tespit edildi. Sonuçlar Box-Behnken tasarımının kültür koşullarının üç faktörünü optimize etmek için yeterli bir yöntem olduğunu gösterdi.

Keywords

Bacillus, Cevap Yüzeyi Yöntemi, Antagonistik etki

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