saujs Sakarya University Journal of Science 2147-835X Sakarya University 10.16984/saufenbilder.341372 Chemical Engineering Food Engineering Kimya Mühendisliği Gıda Mühendisliği An Investigation of Inhibition Effects of Honey, Pollen, Propolis and Royal Jelly Extracts on Thioredoxin Reductase Enzyme Activity Tiyoredoksin Redüktaz Enzim Aktivitesi Üzerine Bal, Polen, Propolis ve Arı Sütü Extraktlarının İnhibisyon Etkilerinin Araştırılması Akkemik Ebru SİİRT ÜNİVERSİTESİ Akbulut Gamze 12 01 2018 22 6 1585 1590 10 02 2017 01 15 2018 Copyright © 1997, Sakarya University Journal of Science 1997 Sakarya University Journal of Science

The thioredoxin reductaseenzyme is an enzyme that prevents the mechanism of apoptosis from working andthus triggers the formation of cancer. Therefore, the inhibition of thethioredoxin reductase enzyme is thought to prevent or inhibit cancer. In thisstudy, the effects of extracts of plateau honey, pine honey, chestnut honey,mad or wild honey, pollen, propolis and royal jelly on thioredoxin reductaseenzyme activity were investigated. Enzyme activities were measured at constantsubstrate and different inhibitor concentrations to calculate IC50values. Over five different inhibitor concentrations were used for measuringthe inhibition constant. Total antioxidant activity were investigated in orderto compare the extracts used in the inhibition study. The strongest inhibitoryeffect was seen in the pollen methanol extract (IC50 = 2.44μg /mL)

Tiyoredoksin redüktaz enzimi,apoptoz mekanizmasının çalışmasını önleyen ve böylece kanser oluşumunutetikleyen bir enzimdir. Bu nedenle tiyoredoksin redüktaz enziminininhibisyonunun kanseri önlediği veya durdurduğu düşünülmektedir. Bu çalışmada, yaylabalının, çam balının, kestane balının, deli veya yabani balının, polenin,propolisin ve arı sütünün ekstraktlarının tioredoksin redüktaz enzim aktivitesiüzerindeki etkileri araştırılmıştır. Enzim aktiviteleri IC50 değerlerinihesaplamak için sabit substrat ve farklı inhibitör konsantrasyonlarında ölçülmüştür.İnhibisyon sabitinin ölçümü için beşten fazla farklı inhibitörkonsantrasyonları kullanılmıştır. İnhibisyon çalışmasında kullanılanekstraktları karşılaştırmak için toplam antioksidan aktivitesi araştırılmıştır.En güçlü inhibisyon etkisi polen metanol ekstraktında (IC50 = 2.44μg/mL) görülmüştür.

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