saujs Sakarya University Journal of Science 2147-835X Sakarya University 10.16984/saufenbilder.356720 Structural Biology Yapısal Biyoloji Screening of Xylanase and Glucose Isomerase Producing Bacteria Isolated from Hot Springs in Turkey Türkiye’de Bulunan Bazı Sıcak Su Kaynaklarından İzole Edilen Bakterilerin Ksilanaz ve Glukoz İzomeraz Üretimi Yönünden Taranması Colak Dilsat Nigar GİRESUN ÜNİVERSİTESİ Inan Bektas Kadriye KARADENİZ TEKNİK ÜNİVERSİTESİ Tokgoz Muslum Canakcı Sabriye KARADENİZ TEKNİK ÜNİVERSİTESİ Belduz Ali Osman KARADENİZ TEKNİK ÜNİVERSİTESİ 12 01 2018 22 6 1804 1811 11 21 2017 07 30 2018 Copyright © 1997, Sakarya University Journal of Science 1997 Sakarya University Journal of Science

Theaim of this study was screening of xylanase and glucose isomerase producingthermophilic bacteria isolated from some hot springs located to Aegean Regionof Turkey. Total sixty eight thermophilic isolates (Anoxybacillus, Brevibacillus,Geobacillus, Aneurinibacillus, Thermus, Paenibacillus and Proteobacter) werecollected previously from these fields and identified based on 16S rDNA genesequences. The isolates were screened with plate assay for determining thexylanase and glucose isomerase production abilities seperately in order to findnew strains for industrial processes. After an incubation period of two daysfor xylanase and 5-6 days for glucose isomerase at 50-60 °C, positive strainswere determined. Enzyme producing strains were confirmed by spectrophotemetricmeasurements with crude enzyme extracts, birchwood xylan and glucose were usedas substrates. Most of the strains (59 strains) were positive for xylandegradation while only sixteen of the strains had showed glucose isomeraseactivity. Fourteen of the strains have showed both xylanase and glucoseisomerase activity. None of the Paenibacillus, Aneurinibacillus, and Proteobacter strains were glucose isomerasepositive, although the glucose isomerase activity of Geobacillus strains were notably high. Both xylanase and glucoseisomerase activities have observed at 50-60 °C which is suitable forbiotechnological applications.

Buçalışmanın amacı Türkiye’nin Ege Bölgesinde bulunan bazı sıcak sukaynaklarından elde edilen termofilik bakterilerin ksilanaz ve glukoz izomerazüretmi yönünden taranmasıdır. Toplam 68 izolat (Anoxybacillus, Brevibacillus,Geobacillus, Aneurinibacillus, Thermus, Paenibacillus ve Proteobacter) dahaönceden bu alanlardan temin edilmiş ve 16S rDNA gen dizilerine göretanımlanmıştır. Öncelikle petri deneyi ile taranan izolatlar, ilgili enzimlereuygun besiyeri içerisinde ksilanaz için iki gün, glukoz izomeraz için ise 5-6gün süreyle 50-60 °C’de inkübasyona tabii tutuldu ve pozitif izolatlarbelirlendi. Enzim ürettiği belirlenen türler doğrulama için spektrofotometrikanalizlere tabi tutuldu, birchwood ksilan ve glukoz substrat olarak kullanıldı.Türlerin çoğunluğu (59 tanesi) ksilanaz aktivitesi yönünden pozitif iken 16türün de glukoz izomeraz aktivitesine sahip olduğu gözlendi. Izolatlardan 14tanesinin her iki enzim yönünden de aktif olduğu belirlendi. Paenibacillus,Aneurinibacillus ve Proteobactertürlerinden hiç bir tanesi glukoz izomeraz aktivitesi göstermezken Geobacillus türlerinin glukoz izomerazaktivitesinin oldukça yüksek olduğu belirlendi. Her iki enzim deaktivitelerini, biyoteknolojik uygulamalar için tercih edilen sıcaklık aralığıolan 50-60 °C’de gösterdi.

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