<?xml version="1.0" encoding="UTF-8"?>
<!DOCTYPE article PUBLIC "-//NLM//DTD JATS (Z39.96) Journal Publishing DTD v1.4 20241031//EN"
        "https://jats.nlm.nih.gov/publishing/1.4/JATS-journalpublishing1-4.dtd">
<article  article-type="research-article"        dtd-version="1.4">
            <front>

                <journal-meta>
                                                                <journal-id>saujs</journal-id>
            <journal-title-group>
                                                                                    <journal-title>Sakarya University Journal of Science</journal-title>
            </journal-title-group>
                                        <issn pub-type="epub">2147-835X</issn>
                                                                                            <publisher>
                    <publisher-name>Sakarya University</publisher-name>
                </publisher>
                    </journal-meta>
                <article-meta>
                                        <article-id pub-id-type="doi">10.16984/saufenbilder.742691</article-id>
                                                                <article-categories>
                                            <subj-group  xml:lang="en">
                                                            <subject>Structural Biology</subject>
                                                    </subj-group>
                                            <subj-group  xml:lang="tr">
                                                            <subject>Yapısal Biyoloji </subject>
                                                    </subj-group>
                                    </article-categories>
                                                                                                                                                        <title-group>
                                                                                                                                                            <article-title>Phytochemical Screening of Bioactive Components of Medicinal Plant Ajuga chamaepitys subsp. laevigata (Banks &amp; Sol.) P.H.Davis and Ajuga bombycina Boiss. by GC-MS Analysis</article-title>
                                                                                                    </title-group>
            
                                                    <contrib-group content-type="authors">
                                                                        <contrib contrib-type="author">
                                                                    <contrib-id contrib-id-type="orcid">
                                        https://orcid.org/0000-0001-6738-7527</contrib-id>
                                                                <name>
                                    <surname>Kaplan</surname>
                                    <given-names>Alevcan</given-names>
                                </name>
                                                                    <aff>BATMAN UNIVERSITY</aff>
                                                            </contrib>
                                                                                </contrib-group>
                        
                                        <pub-date pub-type="pub" iso-8601-date="20201001">
                    <day>10</day>
                    <month>01</month>
                    <year>2020</year>
                </pub-date>
                                        <volume>24</volume>
                                        <issue>5</issue>
                                        <fpage>1053</fpage>
                                        <lpage>1064</lpage>
                        
                        <history>
                                    <date date-type="received" iso-8601-date="20200526">
                        <day>05</day>
                        <month>26</month>
                        <year>2020</year>
                    </date>
                                                    <date date-type="accepted" iso-8601-date="20200808">
                        <day>08</day>
                        <month>08</month>
                        <year>2020</year>
                    </date>
                            </history>
                                        <permissions>
                    <copyright-statement>Copyright © 1997, Sakarya University Journal of Science</copyright-statement>
                    <copyright-year>1997</copyright-year>
                    <copyright-holder>Sakarya University Journal of Science</copyright-holder>
                </permissions>
            
                                                                                                                        <abstract><p>Herbal plants have been a source of food for human beings for many years; they have also frequently been used as an alternative to modern medicine. Because synthetic drugs have possible side effects and are often considerably expensive, understanding how various plants are used for the treatment of specific ailments has become increasingly important. Plant extracts contain multiple active constituents and this has led to the production of new drugs and chemicals derived from the various parts of plants. In Anatolian folk medicine, Ajuga L. (Lamiaceae) species are used by people in many villages and towns for the therapeutic value of their bioactive components. This study was thus designed to examine the possible bioactive components of Ajuga bombycina Boiss. (an endemic species) and Ajuga chamaepitys subsp. laevigata (Banks &amp;amp; Sol.) P.H.Davis. In the study the bioactive components of the GC-MS analysis of dried leaves and flower samples were screened using an Agilent 7890B GC- 5977MSD model with hexane as solvent. Phytochemicals which have a wide range of biological applications and high therapeutic value were found in the samples.</p></abstract>
                                                            
            
                                                                                        <kwd-group>
                                                    <kwd>Medicinal plants</kwd>
                                                    <kwd>  bioactive components</kwd>
                                                    <kwd>  GC-MS analysis</kwd>
                                                    <kwd>  Ajuga L.</kwd>
                                            </kwd-group>
                            
                                                                                                                                                    </article-meta>
    </front>
    <back>
                            <ref-list>
                                    <ref id="ref1">
                        <label>1</label>
                        <mixed-citation publication-type="journal">L. Tripathi and JN. Tripathi, “Role of Biotechnology in Medicinal Plants”, Tropical Journal of Pharmaceutical Research, vol. 2, no. 2, pp.243-253, 2003.</mixed-citation>
                    </ref>
                                    <ref id="ref2">
                        <label>2</label>
                        <mixed-citation publication-type="journal">SO. Odesanmi,  RA. Lawal, SA. Ojokuku,  “Effects of ethanolic extract of Tetrapleura tetraptera on Liver Function Profile and Histopathology in Male Dutch White Rabbits”, International Journal of Tropical Medicine, vol. 4, no. 4, pp.136-139, 2009.</mixed-citation>
                    </ref>
                                    <ref id="ref3">
                        <label>3</label>
                        <mixed-citation publication-type="journal">K. Sheeja, C.  Karthar,  “Activation of cytotoxic Tlymphocyte responses and attenuation of tumor growth in vitro by Andrographis paniculata extract and andrographolide”, Immunopharmacology and Immunotoxicology, vol. 29, pp.81-93, 2007.</mixed-citation>
                    </ref>
                                    <ref id="ref4">
                        <label>4</label>
                        <mixed-citation publication-type="journal">PK. Mukherjee, V. Kumar, PJ. Houjhtor, “Serenity of Indian medicinal Plants for acetyl cholinesterase inhibitory activity”, Phytotherapy Research, vol.21 no. 12, pp. 1142-5, 2007.</mixed-citation>
                    </ref>
                                    <ref id="ref5">
                        <label>5</label>
                        <mixed-citation publication-type="journal">YB. Köse, S. Alan, MB. Mutlu, T. Güner, 	Batı Anadolu Bölgesinde Yayılış Gösteren Ajuga L. Cinsine Ait Taksonların Taksonomik ve Ekolojik Özellikleri”, Bilimsel Araştırma Projesi, Anadolu Üniversitesi, Eskişehir, 2011.</mixed-citation>
                    </ref>
                                    <ref id="ref6">
                        <label>6</label>
                        <mixed-citation publication-type="journal">ZH. Israili, B. Lyoussi, “Ethnopharmacology of the Plants of Genus Ajuga”, Pakistan Journal of Pharmaceutical Sciences, vol.22, no.4, pp. 425-462, 2009.</mixed-citation>
                    </ref>
                                    <ref id="ref7">
                        <label>7</label>
                        <mixed-citation publication-type="journal">A. Güner, S. Aslan, T. Ekim, M. Vural, M. Babaç, “Türkiye Bitkileri Listesi Damarlı Bitkiler”, Nezahat Gökyiğit Botanik Bahçesi ve Flora Araştırmaları Derneği Yayını, İstanbul, 2012.</mixed-citation>
                    </ref>
                                    <ref id="ref8">
                        <label>8</label>
                        <mixed-citation publication-type="journal">PH. Davis, “Flora of Turkey and the East Aegean Islands, Edinburgh”, Edinburgh University Press, vol.7, pp. 42- 52, 1982.</mixed-citation>
                    </ref>
                                    <ref id="ref9">
                        <label>9</label>
                        <mixed-citation publication-type="journal">S. Padmavathy, DN. Nair, and T. Shanthi, “GC-MS Analyses of Bioactive Components in Gaultheria fragratissima Wall, Asian Journal of Pharmaceutical and Clinical Research, vol. 7, no. 1, pp. 83-85, 2014.</mixed-citation>
                    </ref>
                                    <ref id="ref10">
                        <label>10</label>
                        <mixed-citation publication-type="journal">M. Nadiroğlu, L. Behçet, and Çakılcıoğlu U, “An ethnobotanical survey of medicinal plants in Karlıova (Bingöl-Turkey)”, Indian Journal of Traditional Knowledge, vol.18, no. 1, pp. 76-87, 2019.</mixed-citation>
                    </ref>
                                    <ref id="ref11">
                        <label>11</label>
                        <mixed-citation publication-type="journal">NN. Azwanida, “A review on the extraction methods use in medicinal plants, principle, strength and limitation”, Medicinal &amp; Aromatic Plants, vol. 4, no.3, pp. 196, 2015.</mixed-citation>
                    </ref>
                                    <ref id="ref12">
                        <label>12</label>
                        <mixed-citation publication-type="journal">SE. Büyüktuncel, “Gelişmiş Ekstraksiyon Teknikleri I”, Hacettepe Üniversitesi Eczacılık Fakültesi Dergisi, vol. 32, no. 2, pp. 209-242, 2012.</mixed-citation>
                    </ref>
                                    <ref id="ref13">
                        <label>13</label>
                        <mixed-citation publication-type="journal">QD. Do, AE. Angkawijaya, PL. Tran-Nguyen, LH. Huynh, FE. Soetaredjo, S. Ismadji, YH. Ju, “Effect of extraction solvent on total phenol content, total flavonoid content, and antioxidant activity of Limnophila aromatic”, Journal of Food and Drug Analysis, vol. 22, no. 3, pp. 296-302, 2014.</mixed-citation>
                    </ref>
                                    <ref id="ref14">
                        <label>14</label>
                        <mixed-citation publication-type="journal">SB. Iloki-Assanga, LM. Lewis-Lujan, CL. Lara-Espinoza, AA. Gil-Salido, D. Fernandez-Angulo, JL. Rubio-Pino, DD. Haines, “Solvent effects on phytochemical constituent profiles and antioxidant activities, using four different extraction formulations for analysis of Bucida buceras L. and Phoradendron californicum”, Complementary and Alternative Medicine BMC Research Notes, vol. 8, no. 1, pp. 396, 2015.</mixed-citation>
                    </ref>
                                    <ref id="ref15">
                        <label>15</label>
                        <mixed-citation publication-type="journal">O. Sevindik, S. Selli, “Üzüm çekirdek yağı eldesinde kullanılan ekstraksiyon yöntemleri”, Gıda, vol. 42, no. 1, pp. 95-103, 2017.</mixed-citation>
                    </ref>
                                    <ref id="ref16">
                        <label>16</label>
                        <mixed-citation publication-type="journal">J. Drapeau, M. Verdier, D. Touraud, U. Kröckel, M. Geier, A. Rose, W. Kunz, “Effective insect repellent formulation in both surfactantless and classical microemulsions with a long-lasting protection for human beings”, Chemistry &amp; Biodiversity, vol. 6, no.6, pp. 934-47, 2009.</mixed-citation>
                    </ref>
                                    <ref id="ref17">
                        <label>17</label>
                        <mixed-citation publication-type="journal">P. Fowler, K. Smith, J. Young, L. Jeffrey, D. Kirkland, S. Pfuhler, P. Carmichael, “Reduction of misleading (&quot;false&quot;) positive results in mammalian cell genotoxicity assays. I. Choice of cell type”, Mutation Research, vol. 742, no. 1-2, pp.11-25, 2012.</mixed-citation>
                    </ref>
                                    <ref id="ref18">
                        <label>18</label>
                        <mixed-citation publication-type="journal">R. Jeyadevi, T. Sivasudha, A. Ilavarasi, and N. Thajuddin, “Chemical Constituents and Antimicrobial Activity of Indian Green Leafy Vegetable Cardiospermum halicacabum”, Indian Journal of Microbiology, vol. 53, pp. 208-213, 2012.</mixed-citation>
                    </ref>
                                    <ref id="ref19">
                        <label>19</label>
                        <mixed-citation publication-type="journal">S. Santoyo, L. Jaime, M. Plaza, M. Herrero, I. Rodriguez-Meizoso, E. Ibañez, and G. Reglero, “Antiviral compounds obtained from microalgae commonly used as carotenoid sources”, Journal of Applied Phycology, vol. 24, pp. 731-741, 2012.</mixed-citation>
                    </ref>
                                    <ref id="ref20">
                        <label>20</label>
                        <mixed-citation publication-type="journal">A. Maxia, D. Falconieri, A. Piras, S. Porcedda, B. Marongiu, M. Assunta Frau, MJ. Gonçalves, C. Cabral, C. Cavaleiro and L. Salgueiro, “ Chemical Composition and Antifungal Activity of Essential Oils and Supercritical CO2 Extracts of  Apium nodiflorum (L.) Lag.”, Mycopathologia, vol. 174, no. 1, pp. 61-67, 2012.</mixed-citation>
                    </ref>
                                    <ref id="ref21">
                        <label>21</label>
                        <mixed-citation publication-type="journal">RB. Venkata, LA. Samuel, M. Pardha Saradhi, B. Narashimha Rao, A. Naga Vamsi Krishna, M. Sudhakar, TM.  Radha Krishnan, “Antibacterial, antioxidant activity and GC- MS analysis of  Eupatorium odoratum”, Asian Journal of Pharmaceutical and Clinical Research, vol. 5, no.2, pp. 104-105, 2012.</mixed-citation>
                    </ref>
                                    <ref id="ref22">
                        <label>22</label>
                        <mixed-citation publication-type="journal">JSM. Richardson, G. Sethi, G. Serm Lee, and SN. Abdul Malek, “Chalepin: isolated from Ruta angustifolia L. Pers induces mitochondrial mediated apoptosis in lung carcinoma cells”, BMC Complementary and Alternative Medicine, vol. 16, pp. 389, 2016.</mixed-citation>
                    </ref>
                                    <ref id="ref23">
                        <label>23</label>
                        <mixed-citation publication-type="journal">N. Balpınar and G. Okmen,  “Biological Activities and Chemical Composition of Senecio vernalis Growing in the Lakes Region of Turkey”, International Journal of Environmental Science and Technology, vol. 16, pp. 5205-5212, 2018.</mixed-citation>
                    </ref>
                                    <ref id="ref24">
                        <label>24</label>
                        <mixed-citation publication-type="journal">T. Seenivasagan, AV. Paul, “Gas-chromatography and electroantennogram analysis of saturated hydrocarbons of cruciferous host plants and host larval body extracts of Plutella xylostella for behavioural manipulation of Cotesia plutellae”, Indian Journal of Experimental Biology, vol. 49, no. 5, pp. 375-86. 2011.</mixed-citation>
                    </ref>
                                    <ref id="ref25">
                        <label>25</label>
                        <mixed-citation publication-type="journal">S. Mathur, A. Zayeem, S. Kanameni, M. Tibrewal, N. Wadhwa, P. Arora,  and  A.  Kumar, “Effect of Various Concentration of Octacosane, Pentacosane and Tricosane on Foraging Behavior of Trichogrammatids”, International Journal of Scientific and Research Publications, vol. 2, Issue 6, 2012.</mixed-citation>
                    </ref>
                                    <ref id="ref26">
                        <label>26</label>
                        <mixed-citation publication-type="journal">N. Samadi, A. Manayi, M. Vazirian, M. Samadi, Z. Zeinalzadeh, Z. Saghari, N. Abadian, VO. Mozaffarian, M. Khanavi, “Chemical composition and antimicrobial activity of the essential oil of Anthemis altissima L. var. altissima”, Natural Product Research, vol. 26, no. 20, pp.1931-4, 2012.</mixed-citation>
                    </ref>
                                    <ref id="ref27">
                        <label>27</label>
                        <mixed-citation publication-type="journal">Y. Nakashima, MA. Birkett, BJ. Pye, and W. Powell, “Chemically Mediated Intraguild Predator Avoidance by Aphid Parasitoids: Interspecific Variability in Sensitivity to Semiochemical Trails of Ladybird Predators”, Journal of Chemical Ecology, vol. 32, pp. 1989-1998, 2006.</mixed-citation>
                    </ref>
                                    <ref id="ref28">
                        <label>28</label>
                        <mixed-citation publication-type="journal">X. Sun, X. Zhang, G. Wu, X. Li, F. Liu, Z.  Xin, and J. Zhang, “n-Pentacosane Acts as both Contact and Volatile Pheromone in the tea Weevil, Myllocerinus aurolineatus”, Journal of Chemical Ecology, vol. 43, pp. 557- 562, 2017.</mixed-citation>
                    </ref>
                                    <ref id="ref29">
                        <label>29</label>
                        <mixed-citation publication-type="journal">JR. Kuiate, JM. Bessière, PH. Zollo, SP. Kuate, “Chemical composition and antidermatophytic properties of volatile fractions of hexanic extract from leaves of Cupressus lusitanica Mill. from Cameroon”, Journal of Ethnopharmacology, vol. 16, no.103(2), pp. 160-5, 2006.</mixed-citation>
                    </ref>
                                    <ref id="ref30">
                        <label>30</label>
                        <mixed-citation publication-type="journal">S. Kumar, R. Malhotra, D. Kumar, “Euphorbia hirta: Its chemistry, traditional and medicinal uses, and pharmacological activities”, Pharmacognosy Reviews, vol. 4, no. 7, pp.58-61, 2010a.</mixed-citation>
                    </ref>
                                    <ref id="ref31">
                        <label>31</label>
                        <mixed-citation publication-type="journal">MB. Jemia, C. Formisano, S. Bancheva, M. Bruno, F. Senatore, “Chemical composition of the essential oils of Centaurea formanekii and C. orphanidea ssp. thessala, growing wild in Greece”, Natural product communications, vol. 7, no.8, pp. 1083-6, 2012.</mixed-citation>
                    </ref>
                                    <ref id="ref32">
                        <label>32</label>
                        <mixed-citation publication-type="journal">R. Sivasubramanian and P. Brindha, “In-vitro cytotoxic, antioxidant and GC-MS studies on Centratherum punctatum cass.”,  International Journal of Pharmaceutical Sciences, vol. 5, no. 3, pp. 364-367, 2013.</mixed-citation>
                    </ref>
                                    <ref id="ref33">
                        <label>33</label>
                        <mixed-citation publication-type="journal">GG. Grant, D. Frech, L.  MacDonald, KN. Slessor, and King GGS. “Copulation releaser pheromone in body scales of female whitemarked tussock moth, Orgyia leucostigma (Lepidoptera: Lymantriidae): identification and behavioral role”, Journal of Chemical Ecology, vol. 13, pp. 345-356, 1987.</mixed-citation>
                    </ref>
                                    <ref id="ref34">
                        <label>34</label>
                        <mixed-citation publication-type="journal">B. Brei, JD. Edman, B. Gerade, and JM. Clark, “Relative Abundance of Two Cuticular Hydrocarbons Indicates Whether a Mosquito Is Old Enough to Transmit Malaria Parasites”, Journal of Medicinal Entomology, vol. 41, no. 4, pp. 807-809, 2004.</mixed-citation>
                    </ref>
                                    <ref id="ref35">
                        <label>35</label>
                        <mixed-citation publication-type="journal">MB. Zakariaa, Z. Vijayasekarana Ilhama, and NA. Muhamad, “Anti-Inflammatory Activity of Calophyllum inophyllum Fruits Extracts”, Procedia Chemistry, vol. 13, pp. 218-22, 2014.</mixed-citation>
                    </ref>
                                    <ref id="ref36">
                        <label>36</label>
                        <mixed-citation publication-type="journal">YB. Köse, G. Iscan, and B. Demirci,  “Antimicrobial Activity of the Essential Oils Obtained from Flowering Aerial Parts of Centaurea lycopifolia Boiss. et Kotschy and Centaurea cheirolopha (Fenzl) Wagenitz from Turkey”, Journal of Essent Oil Bear Plants, vol. 19, no. 3, pp. 762-768, 2016.</mixed-citation>
                    </ref>
                                    <ref id="ref37">
                        <label>37</label>
                        <mixed-citation publication-type="journal">G. Topçu, G. Kökdil, Z. Türkmen, W. Voelter, E. Adou, and G. David, “A new Clerrodane Diterpene and Other Constituents from Ajuga chamaepitys ssp. laevigata”, Zeitschrift für Naturforschung B, vol.59, pp.1, 2014.</mixed-citation>
                    </ref>
                                    <ref id="ref38">
                        <label>38</label>
                        <mixed-citation publication-type="journal">R. Devika and Justin K, “Screening and Evaluation of  Bioactive Components of Tagetes erecta L. By GC-MS Analysis”, Asian Journal of Pharmaceutical and Clinical Research, vol. 7, no.2, pp. 58-60, 2014.</mixed-citation>
                    </ref>
                                    <ref id="ref39">
                        <label>39</label>
                        <mixed-citation publication-type="journal">A. Ponmathi Sujatha, R. Michael Evanjaline, S. Muthukumarasamy, V. Mohan,  “Determination of Bioactive Components of Barleria Courtallica Nees (Acanthaceae) By Gas Chromatography-Mass Spectrometry Analysis”, Asian Journal of Pharmaceutical and Clinical Research, vol. 10, no. 6, pp. 273-283, 2017.</mixed-citation>
                    </ref>
                                    <ref id="ref40">
                        <label>40</label>
                        <mixed-citation publication-type="journal">G. Rajeswari, M. Murugan, VR. Mohan, “ GC-MS analysis of bioactive components of Hugonia mystax L. (Linaceae)”, Research Journal of Pharmaceutical, Biological and Chemical Sciences, vol. 3, no. 4, pp. 301-308, 2012.</mixed-citation>
                    </ref>
                                    <ref id="ref41">
                        <label>41</label>
                        <mixed-citation publication-type="journal">SN. Chandra Dev, De and D. Kantishree, MW. Khan, “GC-MS Analysis of  Phytochemicals of Methanolic Extract of  Leaves of  Lawsonia inermis Linn.”, Indian Journal of Medical and Pharmaceutical  Sciences, vol. 3, no. 6, pp. 77-82, 2016.</mixed-citation>
                    </ref>
                                    <ref id="ref42">
                        <label>42</label>
                        <mixed-citation publication-type="journal">C. Arunmathi and T. Malarvili, “Analysis of bioactive compounds in methanol extract of Aplotaxis auriculata rhizome using GC-MS”,  Journal of Pharmacognosy and Phytochemistry, vol.6, no. 3, pp. 243-247, 2017.</mixed-citation>
                    </ref>
                                    <ref id="ref43">
                        <label>43</label>
                        <mixed-citation publication-type="journal">AVN. Paul, S. Singh, and AK. Singh, “Kairomonal effect of some saturated hydrocarbons on the egg parasitoids, Trichogramma brasiliensis (Ashmead) and Trichogramma exiguum (Hymenoptera: Trichogrammatidae)”, Journal of Applied Entomology,  vol.126, pp. 409-416, 2002.</mixed-citation>
                    </ref>
                                    <ref id="ref44">
                        <label>44</label>
                        <mixed-citation publication-type="journal">NM. Gumgumjee and SA. Hajar, “Antibacterial activities and GC-MS analysis of phytocomponents of Ehretia abyssinica R.Br. ex fresen”, International Journal of Applied Biology Pharmaceutical Technology, vol. 6, no.2, pp. 236-241, 2015.</mixed-citation>
                    </ref>
                                    <ref id="ref45">
                        <label>45</label>
                        <mixed-citation publication-type="journal">D. Saïdana, MA. Mahjoub, O. Boussaada, J. Chriaa, I. Chéraif, Daami M, Z. Mighri, AN. Helal, “Chemical composition and antimicrobial activity of volatile compounds of Tamarix boveana (Tamaricaceae)”, Microbiological Research, vol. 163, no. 4, pp. 445-55, 2008.</mixed-citation>
                    </ref>
                                    <ref id="ref46">
                        <label>46</label>
                        <mixed-citation publication-type="journal">B. Bakır, A. Him, H. Özbek, E. Düz, M. Tütüncü, “Investigation of the Anti-inflammatory and Analgesic Activities of β-caryophyllene”, International Journal of Essential Oil Therapeutics, vol. 2, pp. 41-44, 2008.</mixed-citation>
                    </ref>
                                    <ref id="ref47">
                        <label>47</label>
                        <mixed-citation publication-type="journal">JP. Pinho, AS.  Silva, BG. Pinheiro, I. Sombra, C. Bayma Jde, S. Lahlou, PJ. Sousa, PJ. Magalhães, “Antinociceptive and antispasmodic effects of the essential oil of Ocimum micranthum: potential anti-inflammatory properties”, Planta Medica, vol. 78, no. 7, pp.681-5, 2012.</mixed-citation>
                    </ref>
                                    <ref id="ref48">
                        <label>48</label>
                        <mixed-citation publication-type="journal">Anonymous.[internet].Availablefromhttps://pubchem.ncbi.nlm.nih.gov/compound/trans-Caryophyllene, [cited 2020 April 09], 2020.</mixed-citation>
                    </ref>
                                    <ref id="ref49">
                        <label>49</label>
                        <mixed-citation publication-type="journal">KHC. Baser, M. Kürkcüoğlu, and FZ. Erdemgil, “The Essential Oil of Ajuga bombycina from Turkey”, Chemistry of  Natural Compounds, vol.37, pp. 3, 2001.</mixed-citation>
                    </ref>
                                    <ref id="ref50">
                        <label>50</label>
                        <mixed-citation publication-type="journal">MK. Swamy, G. Arumugam, R. Kaur, A. Ghasemzadeh, MM. Yusoff, and UR. Sinniah,”GC-MS. Based Metabolite Profiling, Antioxidant and Antimicrobial Properties of Different Solvent Extracts of Malaysian Plectranthus amboinicus Leaves”, Evidence-Based Complementary and Alternative Medicine,  2017.https://doi.org/10.1155/2017/1517683.</mixed-citation>
                    </ref>
                                    <ref id="ref51">
                        <label>51</label>
                        <mixed-citation publication-type="journal">A. Yaşar, O. Üçüncü, C.  Güleç, H. İnceer, S. Ayaz, and N. Yaylı, “GC-MS Analysis of Chloroform Extracts in Flowers, Stems, and Roots of Tripleurospermum callosum”, Pharmaceutical Biology, vol. 43, no. 2, pp. 108-112, 2005.</mixed-citation>
                    </ref>
                                    <ref id="ref52">
                        <label>52</label>
                        <mixed-citation publication-type="journal">D. Gomathi, M. Kalaiselvi, G. Ravikumar, K. Devaki, C. Uma, “GC-MS analysis of bioactive compounds from the whole plant ethanolic extract of Evolvulus alsinoides (L.) L.”, Journal of  Food Science and Technology, vol. 52, no. 2, pp.1212- 1217, 2015.</mixed-citation>
                    </ref>
                                    <ref id="ref53">
                        <label>53</label>
                        <mixed-citation publication-type="journal">Ö. Kılıç, “Marrubium parviflorum subsp. parviflorum Bitkisinin Yağ Asidi ve Uçucu Yağ Kompozisyonu”, Mus Alparslan University Journal of Science, vol. 6, no. 1, pp. 487-491, 2018.</mixed-citation>
                    </ref>
                                    <ref id="ref54">
                        <label>54</label>
                        <mixed-citation publication-type="journal">P. Kumar, PS. Kumaravel, and  C. Lilitha,  “Screening of Antioxidant, Total Phenolic and GC-MS study of Vitex negundo”, Africa Journal of Biochemistry and Research, vol. 4, no.7, pp.191-195, 2010b.</mixed-citation>
                    </ref>
                                    <ref id="ref55">
                        <label>55</label>
                        <mixed-citation publication-type="journal">P. Kalaisezhiyen, V. Sasikumar, “GC-MS evaluation of Chemical constituents from methanolic leaf extract of Kedrostis foetidissima (Jacq.) Cogn.”, Asian Journal of Pharmaceutical and Clinical Research, vol. 5, no. 4, pp. 77-81, 2012.</mixed-citation>
                    </ref>
                                    <ref id="ref56">
                        <label>56</label>
                        <mixed-citation publication-type="journal">M. Sermakkani and V. Thangapandian, “GC-MS analysis of Cassia italica leaf methanol extract”, Asian Journal of Pharmaceutical Clinic Research, vol. 5, no. 2, pp. 90-94, 2012.</mixed-citation>
                    </ref>
                                    <ref id="ref57">
                        <label>57</label>
                        <mixed-citation publication-type="journal">MI. Bello, SB. Ishidi, and IY. Sudi, “Phytochemical Screening and Antimicrobial Activity of Ethanolic and Aqueous Stembark Extracts of Boswellia dalzielli (Hutch)”,  Asian Journal of Biochemical and Pharmaceutical, vol. 1, no. 3, pp. 194- 198, 2013.</mixed-citation>
                    </ref>
                                    <ref id="ref58">
                        <label>58</label>
                        <mixed-citation publication-type="journal">K.Shibula, S. Velavan, “Determination of Phytocomponents in Methanolic Extract of Annona muricata Leaf Using GC-MS Technique”,  International Journal of Pharmacognosy and Phytochemical Research, vol. 7, no.6, pp. 1251-1255, 2015.</mixed-citation>
                    </ref>
                                    <ref id="ref59">
                        <label>59</label>
                        <mixed-citation publication-type="journal">P. Powar and D. Gaikwad,  “GC-MS Analysis of Bioactive Compounds of Aegiceras corniculatum Bark.”, Indian Journal of Plant Sciences, vol. 5, no. 3, pp. 13-17, 2016.</mixed-citation>
                    </ref>
                                    <ref id="ref60">
                        <label>60</label>
                        <mixed-citation publication-type="journal">A. Omoregbeee, M. Idu, “GC-MS analysis of ethanolic extract of Boswellia dalzieliihutch (Burseraceae) root from Nigeria”, Chemistry Research Journal, vol. 2, no. 2, pp. 33-38. 2017.</mixed-citation>
                    </ref>
                                    <ref id="ref61">
                        <label>61</label>
                        <mixed-citation publication-type="journal">P. Yamuna, P.Abirami, P. Vijayashalini, and M. Sharmila, “GC-MS analysis of bioactive compounds in the entire plant parts of ethanolic extract of Gomphrena decumbens Jacq.”, Journal of Medicinal Plants Studies, vol. 5, no.3, pp. 31-37, 2017.</mixed-citation>
                    </ref>
                            </ref-list>
                    </back>
    </article>
