Hura crepitans (Sandbox tree) of the family ofEuphorbiaceae has enjoyed many ethnomedicinal applicationsbut little is known about its chemistry and pharmacology. Thisresearch reports the in vitro antioxidant activity of this plantusing two different assay methods. Scavenging effect on 2,2-diphenylpicrylhydrazyl (DPPH) radical at 517 nm and onhydroxyl radical generated by hydrogen peroxide at 285 nm ina UV-Visible spectrophotometric assay. Butylatedhydroxyanisole(BHA), vitamin C and α-tocopherol were used as referencestandards. There is generally decrease in absorption of DPPHcaused by the extracts. The percent inhibition of the crudeextract increases with a decrease in concentration in the DPPHphotometric assay. The percent inhibition of hexane, ethylacetateand butanol fractions was low except for the butanol fraction(50.7 % at 1.0 mg/ml) when compared with standards vitaminC (90.8 % at 1.0 mg/ml) and BHA (95.4 % at 1.0 mg/ml). In thehydrogen peroxide assay however, the hexane, ethylacetate andbutanol fractions scavenged hydroxyl radical more effectivelythan the standards. The crude extract possessed maximum %inhibition in both the DPPH and hydrogen peroxide free radicals.Therefore, H. crepitans has very weak activity as a hydrogendonor but its activity as hydroxyl radical scavenger is high whencompared to standards used. This study suggests that the crudeand fractions obtained from H. crepitans possess antioxidantactivities which can counteract or prevent oxidative damage inbiological systems caused by the presence of hydroxyl radical.