Asemptomatik Tam Gömük Alt Yirmi Yaş Dişlerinin Foliküllerinde Ki-67 ve p53 Ekspresyonunun Değerlendirilmesi

Year 2017, Volume: 8 Issue: 3, 1 - 7, 20.12.2017

Ender Burhanoğlu Müge Çına Aksoy Pınar Aslan Koşar

https://doi.org/10.22312/sdusbed.295857

Abstract

AMAÇ:
Gömük diş çekimi, özellikle en sık gömük kalan dişler olan alt yirmi yaş
dişlerinin çekimi, en çok gerçekleştirilen oral cerrahi işlemdir. Asemptomatik
tam gömük alt yirmi yaş dişlerinin çekimi konusunda net bir görüş birliğine
varılamamıştır. Ki-67, farklı hücre populasyonlarının büyüme hızını yani
proliferasyon indeksini belirlemede kullanılmaktadır. p53 ise tümör baskılayıcı
bir proteindir. Sağlıklı dokuların tersine, tümör hücrelerinde p53 proteininin
ekspresyonu artmaktadır. Bu çalışmanın amacı, sigara kullanan ve kullanmayan
bireylerin, asemptomatik tam gömük alt yirmi yaş dişlerinin perikoronal
foliküllerinde p53 ve Ki-67 proteinlerinin ekspresyonlarının
karşılaştırılmasıdır.

MATERYAL
METOD: Çalışmaya 50 sigara kullanan, 50 sigara kullanmayan 100 hastadan,
asemptomatik tam gömük alt yirmi yaş dişlerinin perikoronal folikülleri dahil
edilmiştir. Dokularda, p53 ve Ki-67 antikorları ile immunohistokimyasal boyama
yapılarak immünhistokimyasal olarak, hücre çekirdeği boyanan hücre sayısı ve
boyanma yoğunlukları skorlanarak değerlendirilmiştir.

BULGULAR:
Çalışmamızda, sigara kullanan ve kullanmayan hastaların perikoronal
foliküllerinde, Ki-67 ve p53 proteinlerinin boyanması “negatif” olarak bulundu.

SONUÇ:
Çalışmamızın sonuçlarına göre, her ne kadar sigaranın genel sağlık açısından
bir risk faktörü oluşturduğu biliniyor olsa da, asemptomatik tam gömük dişlerin
patolojik dönüşüm riskini arttırdığına dair net bir kanıt bulunamamıştır. Gömük
alt yirmi yaş dişlerinin çekimine, detaylı klinik ve radyografik muayene ile
birlikte hastanın sosyokültürel düzeyi göz önünde bulundurularak, karar
verilmesi uygun bir seçenek olabilir.

Keywords

Dental folikül, immunohistokimya, Ki-67, p53

References

• 1. Adeyemo WL. Do pathologies associated with impacted lower third molars justify prophylactic removal? A critical review of the literature. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2006;102(4):448-52.
• 2. Regezi JA, Sciubba JJ, Jordan RC. Oral pathology: clinical pathologic correlations: Elsevier Health Sciences; 2012.
• 3. Ozarslan SK, Baykul T, Basak K, Kocer G, Tuzum S. Detection of epidermal growth factor receptor intensity in asymptomatic fully impacted lower third molar follicles of smoking and nonsmoking patients. J Craniofac Surg. 2013;24(2):435-8.
• 4. de Oliveira Semenzati G, de Souza Salgado B, Rocha NS, Michelin Matheus SM, de Carvalho LR, Garcia Martins RH. Histological and immunohistochemical study of the expression of p53 and ki-67 proteins in the mucosa of the tongue, pharynx and larynx of rats exposed to cigarette smoke. Inhalation toxicology. 2012;24(11):723-31.
• 5. Prasad SHVR. Expression of p53 protein and ki-67 antigen in oral premalignant leions an oral squamous cell carcinomas: An immunohistochemical study. Natil J Maxillofac Surg. 2011;2(1):38-46.
• 6. Brown D, Gatter K. Ki67 protein: the immaculate deception? Histopathology. 2002;40(1):2-11.
• 7. Bourdon J-C. p53 Family isoforms. Current pharmaceutical biotechnology. 2007;8(6):332.
• 8. Meert AP, Feoli F, Martin B, Verdebout JM, Mascaux C, Verhest A, et al. Ki67 expression in bronchial preneoplastic lesions and carcinoma in situ defined according to the new 1999 WHO/IASLC criteria: a preliminary study. Histopathology. 2004;44(1):47-53.
• 9. Chuang SK, Perrott DH, Susarla SM, Dodson TB. Age as a risk factor for third molar surgery complications. Journal of oral and maxillofacial surgery. J Oral Maxillofac Surg. 2007;65(9):1685-92.
• 10. Villalba L, Stolbizer F, Blasco F, Mauriño NR, Piloni MJ, Keszler A. Pericoronal follicles of asymptomatic impacted teeth: a radiographic, histomorphologic, and immunohistochemical study. International journal of dentistry. 2012; Article ID 935310, 6 pages.
• 11. Saravana GH, Subhashraj K. Cystic changes in dental follicle associated with radiographically normal impacted mandibular third molar. Br J Oral Maxillofac Surg. 2008;46(7):552-3.
• 12. Wali GG, Sridhar V, Shyla HN. A study on dentigerous cystic changes with radiographically normal impacted mandibular third molars. J Maxillofac Oral Surg.2012;11(4):458-65.
• 13. Cabbar F, Guler N, Comunoglu N, Sencift K, Cologlu S. Determination of potential cellular proliferation in the odontogenic epithelia of the dental follicle of the asymptomatic impacted third molars. J Oral Maxillofac Surg. 2008;66(10):2004-11.
• 14. Takeda KS, Hirayama Y, Hirano M, Tanuma JI, Semba I. Immunohistological evaluation of Ki-67, p63, CK19 and p53 expression in oral epithelial dysplasias. J Oral Pathol Med 2006;35(6):369-75.
• 15. Gerdes J, Lemke H, Baisch H, Wacker H-H, Schwab U, Stein H. Cell cycle analysis of a cell proliferation-associated human nuclear antigen defined by the monoclonal antibody Ki-67. J Immunol. 1984;133(4):1710-5.
• 16. MacCallum DE, Hall PA. The location of pKi67 in the outer dense fibrillary compartment of the nucleolus points to a role in ribosome biogenesis during the cell division cycle. J Pathol. 2000;190(5):537-44.
• 17. Bullwinkel J, Baron‐Lühr B, Lüdemann A, Wohlenberg C, Gerdes J, Scholzen T. Ki‐67 protein is associated with ribosomal RNA transcription in quiescent and proliferating cells. J Cell Physiol. 2006;206(3):624-35.
• 18. Nylander K, Dabelsteen E, Hall PA. The p53 molecule and its prognostic role in squamous cell carcinomas of the head and neck. J Oral Pathol Med. 2000;29(9):413-25.
• 19. Ebrahimi M, Boldrup L, Coates PJ, Wahlin Y-B, Bourdon J-C, Nylander K. Expression of novel p53 isoforms in oral lichen planus. Oral oncology. 2008;44(2):156-61.
• 20. Nasser W, Flechtenmacher C, Holzinger D, Hofele C, Bosch FX. Aberrant expression of p53, p16INK4a and Ki‐67 as basic biomarker for malignant progression of oral leukoplakias. J Oral Pathol Med. 2011;40(8):629-35.
• 21. Slade N, Moll U. Mutational Analysis of p53 in Human Tumors. In: Deb S, Deb S, editors. p53 Protocols. Methods in Molecular Biology. 234: Springer New York; 2003. p. 231-43.
• 22. Shetty S, Krishnapillai R, Prabhu S. Assessment and comparison of p53 and p63 expression in oral epithelial dysplasia and squamous cell carcinoma. SRM J Res Dent Sci. 2014;5(3):149.
• 23. Kumar P, Kane S, Rathod GP. Coexpression of p53 and Ki 67 and lack of c-erbB2 expression in oral leukoplakias in India. Braz Oral Res. 2012;26(3):228-34.
• 24. Baweja P, Monappa V, Krishnanand G. P53 Immunohistochemical Staining Patterns in Benign, Premalignant and Malignant Lesions of the Oral Cavity: A Study of 68 Cases. J Interdiscip Histopathol. 2013;1(3):113-20.
• 25. Levine AJ. p53, the Cellular Gatekeeper for Growth and Division. Cell. 1997;88(3):323-31.
• 26. Lane DP. Cancer. p53, guardian of the genome. Nature. 1992;358(6381):15-6.
• 27. Prives C, Hall PA. The p53 pathway. J Pathol. 1999;187(1):112-26.
• 28. Kubbutat MHG, Vousden KH. Keeping an old friend under control: regulation of p53 stability. Molecular Medicine Today. 1998;4(6):250-6.
• 29. Slootweg PJ. p53 protein and Ki‐67 reactivity in epithelial odontogenic lesions. An immunohistochemical study. J Oral Pathol Med. 1995;24(9):393-7.
• 30. Baas IO, Van Den Berg FM, Mulder JWR, Clement MJ, Slebos RJ, Hamilton SR, et al. Potential False‐Positive Results With Antigen Enhancement For Immunohistochemistry of the p53 Gene Product in Colorectal Neoplasms. J Pathol. 1996;178(3):264-7.
• 31. Takami H, Yoshida A, Fukushima S, Arita H, Matsushita Y, Nakamura T, et al. Revisiting TP53 mutations and immunohistochemistry–a comparative study in 157 diffuse gliomas. Brain Pathology. 2015;25(3):256-65.
• 32. Toptaş O, Baykul T, Başak K. Does smoking affect the Ki67 and p53 expressions in asymptomatic fully impacted lower third molar follicles? J Oral Maxillofac Surg. 2015;73(5):819-26.
• 33. Edamatsu M, Kumamoto H, Ooya K, Echigo S. Apoptosis-related factors in the epithelial components of dental follicles and dentigerous cysts associated with impacted third molars of the mandible. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2005;99(1):17-23.