med j sdu

Medical Journal of Süleyman Demirel University

2602-2109

Süleyman Demirel University

Dentistry

Diş Hekimliği

Clinicopathologic significance of high CD133 expression in colorectal carcinomas

Kolorektal kanserlerde yüksek CD133 ekspresyonunun klinikopatolojik önemi

Devrim

Tuba

06 30 2015

22 2 32 38 04 16 2015

1994

Medical Journal of Süleyman Demirel University

Background: To investigate the prognostic significance of CD133 expression in colorectal carcinomas (CRC). Material and Method: CD133 expression was investigated immunohistochemically on the paraffin blocks of 70 sporadic colorectal carcinoma cases which were diagnosed at the department of pathology in Suleyman Demirel University. Low positivity was defined as positive immunoreactivity in < 50% of neoplastic glands, and high positivity was defined as positive immunoreactivity in ≥ 50% of neoplastic glands. Furthermore, the relation of CD133 immunohistochemical expression with pathologic parameters and survival rates were investigated. Results: CD133 expression was detected in 82.9% of the examined cases and high CD133 expression was significantly associated with poorer disease free survival (p=0.004). CD133 expression was significantly higher in tumors located in rectosigmoidal region (p=0.005) and showed a significant negative correlation with histologic grade (p=0.020). Conclusion: In the present study none of the poorly differentiated tumors showed high CD133 positivity probably due to the lack of luminal areas in poorly differentiated areas of the tumor, which is in consistence with the previously published data. Our results indicate that immunohistochemical CD133 expression, which correlates with poorer disease free survival, may be an independent prognostic marker in colorectal carcinoma. The findings of this study support the cancer stem cell model, and reveal the clinical importance of CD133 in CRC cases and present a strong association between high CD133 expression and disease-free survival

Amaç: Kolorektal karsinomlarda (CRC) CD133 ekspresyonunun prognostik önemininbelirlenmesi hedeflendi. Materyal ve Metot: Süleyman Demirel Üniversitesi PatolojiAnabilim Dalı’nda tanı almış sporadik kolorektal kanserli 70 olguya ait CD133ekspresyonları, parafin bloklarda immunohistokimyasal olarak araştırıldı. Neoplazikbezlerin % 50’sinden azında boyanma düşük pozitif; % 50 ve daha fazlasında boyanmayüksek pozitif imünoreaktivite olarak değerlendirildi. Ayrıca, patolojik parametreler vesağ kalım oranları ile CD133 immünhistokimyasal ekspresyon düzeyleri arasındaki ilişkiaraştırıldı. Bulgular: CD133 ekspresyonu incelenen olguların % 82.9’unda tespit edildi veyüksek CD133 düzeyi anlamlı (p = 0.004) derecede kötü hastalıksız sağkalım ile ilişkilibulundu. CD133 ifadesi rektosigmoid bölgede bulunan tümörlerde anlamlı (p = 0.005)derecede yüksekti ve histolojik grade ile anlamlı (p = 0.020) negatif korelasyon gösterdi.Sonuç: Daha önceden yayınlanmış verilerle uyumlu olarak muhtemelen tümörün kötüdiferansiye alanlarındaki luminal bölgelerin eksikliğine bağlı olarak bu çalışmadaki kötüdiferensiye tümörlerde yüksek CD133 pozitifliği belirlenmedi. Sonuçlarımız kötü hastalıksızsağkalım ile korelasyon gösteren immünohistokimyasal CD133 ekspresyonunun kolorektalkarsinoma için bağımsız bir prognostik belirleyici (markır) olabileceğini göstermektedir.Bu çalışmanın bulguları, kanser kök hücre modelini desteklemekte ve CRC olgularındaCD133’ün klinik önemini ve yüksek CD133 ekspresyonu ile hastalıksız sağkalım arasındagüçlü bir ilişki olduğunu ortaya koymaktadır.

CD133 colon cancer immunohistochemistry prognosis

CD133 kolon kanseri immünohistokimya prognoz

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